Wyniki wyszukiwania

Sortuj według:

Ogranicz wyniki do:

Effects of salt stress on physio-biochemical characters and gene expressions in halophyte grass Leptochloa fusca (L.) Kunth

100%

Mohammadi F., Kavousi H. R., Mansouri M.

Acta Physiologiae Plantarum

2019

tom 41

nr 08

Kallar grass (Leptochloa fusca) is a perennial C4 halophytic species with high salt tolerant. The present research was made to investigate the physio-biochemical characters and transcriptional changes of L. fusca under varying salinity levels (0–600 mM NaCl). The Na⁺ level in shoots and roots increased significantly, whereas the K⁺ content was maintained high in 300 mM NaCl and then declined with increasing salinity in both tissues. The content of proline in seedlings exposed to extreme salinity level was 15.5-fold higher than control. Photosynthetic pigments, total soluble proteins, PAL activity, and total phenolic compounds in salt-stressed plants increased gradually up to 450 mM and declined at 600 mM NaCl. High salt concentration led to oxidative stress that was manifested by increased MDA level. To tackle with oxidative damages, L. fusca enhanced the activity of antioxidant enzymes like superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX). Moreover, under NaCl stress, the mRNA levels of SOS1, PM H⁺-ATPase, and NHX1 were up-regulated in both tissues, but higher in roots than in shoots. Our results demonstrate that L. fusca could use an osmotic adjustment, antioxidant defense system, and regulating the ion homeostasis as the most effective salt tolerance mechanisms for better plant growth under saline conditions.

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Molecular characterization and seroprevalence of Echinococcus granulosus in wild boars (Sus scrofa) in south-western Iran

100%

Sarkari B., Mansouri M., Khabisi S. A., Mowlavi G.

Annals of Parasitology

2015

tom 61

nr 4

This study presents the first molecular and serological evaluation of Echinococcus granulosus infections in wild boars in Iran. Twenty five wild boars were collected in south-western Iran, during authorized hunting program, from March to October 2013, necropsied and examined for E. granulosus infection. Furthermore, seroprevalence of cystic echinococcosis in hunted boars was evaluated by an ELISA system. A fertile hydatid cyst due to E. granulosus was detected in the lung of one of the animals. Genotype analysis of the isolate was determined by analyzing a mitochondrial gene, cytochrome C oxidase subunit 1 (co1). DNA was extracted from the cyst sample and polymerase chain reaction amplification and DNA sequencing of the specific region of the co1 gene was performed. Molecular evaluation confirmed the presence of a sheep strain, the G1 genotype, in the wild boar in south-western Iran. This is the first report of the presence of G1 genotype of E. granulosus in wild boar in Iran. Serological evaluation of hydatid cyst by antigen-B ELISA revealed E. granulosus antibodies in 5 (20%) of 25 wild boars. A statistically significant difference was observed between the prevalence of E. granulosus antibodies and gender while the difference between the seroprevalence of E. granulosus and age was insignificant. Findings of this study might have important implications for the prevention and control of cystic echinococcosis.

Ograniczanie wyników

1 Acta Physiologiae Plantarum

1 Annals of Parasitology

2 Mansouri M.

1 Kavousi H. R.

1 Khabisi S. A.

1 Mohammadi F.

1 Mowlavi G.

1 Sarkari B.

1 2019

1 2015

Wyniki wyszukiwania

Effects of salt stress on physio-biochemical characters and gene expressions in halophyte grass Leptochloa fusca (L.) Kunth

Molecular characterization and seroprevalence of Echinococcus granulosus in wild boars (Sus scrofa) in south-western Iran