Wyniki wyszukiwania

Sortuj według:

Ogranicz wyniki do:

Water limitation mitigates high-temperature stress injuries in grapevine cultivars through changes in photosystem II efficiency and antioxidant enzyme pathways

100%

Zha Q., Xi X., He Y., Jiang A., Fang X.

Acta Physiologiae Plantarum

2019

tom 41

nr 06

Plants, via physiological and molecular processes, respond to unsuitable environmental conditions, resulting in stress tolerance. Most previous studies have focused on plant responses to a single abiotic stress, but the effects of combined water deficit and high temperature stresses are more severe and complex than those due to a single stress. Therefore, our study aimed to explore the differences in the damage caused by combined vs. single stresses. Grapevines were subjected to water deficit, high temperature, and water deficit plus high temperature treatments. The transcript levels of heat- and drought-stress genes, activities of photosystem II (PS II) and antioxidant enzymes (superoxide dismutase, catalase, and peroxidase), and changes in abscisic acid (ABA) biosynthesis were evaluated. The activities of PS II and antioxidant enzymes were lower under the water deficit plus high temperature treatment than under the heat treatment alone. The concentration of ABA and the transcript levels of ABA biosynthesis-related genes increased under both types of stress. The enhanced thermo-tolerance observed under drought stress could be attributed to increased PS II efficiency, as well as to changes in antioxidant pathways, mediated by a common regulatory system or including a substantial cross talk between heat- and drought-stress signaling.

Cloning and characterization of MxHA7, a plasma membrane H+ - ATPase gene related to high tolerance of Malus xiaojinensis to iron deficiency

100%

Zha Q., Zhang Q., Zhang X., Han Z., Wang Y.

Acta Physiologiae Plantarum

2014

tom 36

nr 04

Malus xiaojinensis, an iron-efficient apple rootstock, was used to study the molecular mechanisms of iron uptake. Increased H⁺ extrusion under iron-deficient conditions has been related with H⁺-ATPases. Thus, a 2,901-bp plasma membrane H⁺-ATPase gene, MxHA7, encoding 966 amino acids was isolated. Quantitative realtime PCR showed that MxHA7 was specifically induced in the roots of M. xiaojinensis during iron-deficient conditions, not in M. baccata. A functional complementation assay indicated that the high tolerance of MxHA7-transgenic aha7 Arabidopsis thaliana (HA7) plants to iron deficiency was significantly enhanced. Under iron-deficient conditions, Fe²⁺ contents in the roots and chlorophyll concentrations in the leaves of HA7 plants were increased up to about 2 to 3 times compared to Col-0, aha7 and empty vector (EV) (aha7 transformed with an empty vector) plants. The zinc and manganese contents in the roots of HA7 plants were also higher significantly than in aha7 and EV plants under iron-deficient conditions. Meantime, the HA7 plants have less increasing for iron uptake-related genes than those Col-0, aha7 and EV other plants after iron deficiency, which means MxHA7 gene apparently contributed to help Arabidopsis tolerance to iron deficiency.

Ograniczanie wyników

2 Acta Physiologiae Plantarum

2 Zha Q.

1 Fang X.

1 Han Z.

1 He Y.

1 Jiang A.

1 Wang Y.

1 Xi X.

1 Zhang Q.

1 Zhang X.

1 2019

1 2014

Wyniki wyszukiwania

Water limitation mitigates high-temperature stress injuries in grapevine cultivars through changes in photosystem II efficiency and antioxidant enzyme pathways

Cloning and characterization of MxHA7, a plasma membrane H+ - ATPase gene related to high tolerance of Malus xiaojinensis to iron deficiency