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1

The responses of the enzymes related with ascorbate–glutathione cycle during drought stress in apple leaves

100%
Ma Y.-H., Ma F.-W., Wang Y.-H., Zhang J.-K.
Acta Physiologiae Plantarum
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2011
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tom 33
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nr 1
To explore the significance of the ascorbate–glutathione cycle under drought stress, the leaves of 2-yearold potted apple (Malus domestica Borkh.) plants were used to investigate the changes of each component of the ascorbate–glutathione cycle as well as the gene expression of dehydroascorbate reductase (DHAR, EC 1.8.5.1), ascorbate peroxidase (APX, EC 1.11.1.11) and glutathione reductase (GR, EC 1.6.4.2) under drought stress. The results showed that the malondialdehyde (MDA) and H₂O₂ concentrations in apple leaves increased during drought stress and began to decrease after re-watering. The contents of total ascorbate, reduced ascorbic acid (AsA), total glutathione and glutathione (GSH) were obviously upregulated in apple leaves when the soil water content was 40–45%. With further increase of the drought level, the contents of the antioxidants and especially redox state of AsA and GSH declined. However, levels of them increased again after re-watering. Moreover, drought stress induced significant increase of the activities of enzymes such as APX, scavenging H₂O₂, and also of monodehydroascorbate reductase (MDHAR, EC 1.6.5.4), DHAR and GR used to regenerate AsA and GSH, especially when the soil water content was above 40–45%. During severe drought stress, activities of the enzymes were decreased and after rewatering increased again. Gene expression of cytoplasmic DHAR, cytoplasmic APX and cytoplasmic GR showed similar changes as the enzyme activities, respectively. The results suggest that the ascorbate–glutathione cycle is upregulated in response to drought stress, but cannot be regulated at severe drought stress conditions.
2

Studying soil salinity and its relations with microtopography and vegetation at field scale

88%
Zhao C.-W., Xu L., Wang Z.-C., Wang Y.-H., Chi C.-M.
Polish Journal of Environmental Studies
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2013
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tom 22
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nr 4
Spatial variation of soil salinity and sodicity is a typical characteristic of Songnen Plain grasslands in northeast China. Best management practices and grassland productivity improvement require further understanding of the relationships among the causal factors at field scale. A field soil survey for the interactions among salinity, microtopography, and vegetation was conducted on a 2.8 hectare saline sodic grassland at Da’an Sodic Land Experimental Station, Chinese Academy of Sciences, northeast China. Salinity of the soil decreases by depth increment with Na+ and HCO-3 as the dominant ions. Soil salinity and sodicity parameters measured include pH, electrical conductivity for 1 to 5 soil-water extracts (EC1:5), apparent electrical conductivity (ECa), sodium adsorption ratio, and exchangeable sodium percentage. Soil salinity is in an order of slope > mound > depression for microtopography and soil under S. corniculata community is in severe salinity, while there is no significant difference under P. australis-A. mongolica and P. australis communities. The water and salt regime in various microtopographical conditions also is discussed.
3

Predictive value of plasma parameters in the risk of postpartum ketosis in dairy cows

76%
Cao Y., Zhang J., Yang W., Xia C., Zhang H.-Y., Wang Y.-H., Xu C.
Journal of Veterinary Research
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2017
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tom 61
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nr 1
4

Exogenous application of gibberellic acid and ascorbic acid improved tolerance of okra seedlings to NaCl stress

76%
Wang Y.-H., Zhang G., Chen Y., Gao J., Sun Y.-R., Sun M.-F., Chen J.-P.
Acta Physiologiae Plantarum
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2019
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tom 41
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nr 06
Accumulation of salts in soils has become a serious environmental threat for plant growth and causes considerable loss in yield. Okra Abelmoschus esculentus L. is an important crop and sensitive to saline stress. In the present study, to explore methods for growing okra in salty area, exogenous gibberellic acid (GA₃) and ascorbic acid (AsA) were foliage applied on okra seedling under NaCl stress. The results showed that treatment with 100 mM NaCl decreased shoot length, root length, fresh weight, dry weight, contents of chlorophyll pigments and nutrient elements, enhanced levels of electrolyte leakage, H₂O₂, lipid peroxidation and activities of antioxidant enzymes. Treatments with 0.1 mM GA₃ and/or 0.1 mM AsA could alleviated harmful effects of saline stress on okra seedlings by improving growth indicators, increasing contents of chlorophyll and carotenoids, stimulating activities of antioxidant enzymes and decreasing electrolyte leakage, H₂O₂ content and lipid peroxidation. Moreover, concentrations of K, Ca, Mg and Fe in leaves and roots as well as levels of osmo-protectants (proline and soluble protein) increased in response to treatment with GA₃ + AsA in NaCl-stressed okra seedling. Overall, foliar application of GA₃ and/or AsA demonstrated benefits to okra seedlings in salty environments. Combined application of GA₃ and AsA was more effective than sole use of GA₃ or AsA alone.
5

Serum paraoxonase as an indicator for fatty liver in sheep

76%
Cao Y., Zhang J., Yang W., Xia C., Zhang H.-Y., Wang Y.-H., Xu C.
Journal of Veterinary Research
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2017
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tom 61
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nr 1
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