Nuclear magnetic resonance (NMR) spectroscopy was used to study molecular changes during storing and forming spatial network in concentrated starch solution in D₂O. The concentration and temperature effect in chemical shift of proton and car bon signals in NMR spectra allowed to determine the thermodynamic parameters of helix-coil transition.
In the last decade, the development and application of molecular techniques has revolutionised the diagnosis and monitoring of human diseases. Nucleic acids techniques, such as microbial DNA genotyping, analysis of restriction fragment length polymorphism (RFLP) and polymerase chain reaction (PCR), are making progress in medical diagnostic laboratories. Sequence analysis of amplified DNA allows better identification of pathogen, detection of mutant genes and more accurate prognosis of certain diseases.