An analysis of meiotic and mitotic chromosomes of P. corneus inhabiting Lake Kortowskie was made in order to verify the use of different tissues and colchicine treatments, the hypotonization time and two methods of chromosome slide preparation. In total, 30 chromosomal slides of six individuals were analyzed. The well spread chromosomes were introduced onto the slides by dropping a cell suspension of the mantle epithelium, foot and intestine of each individual, directly injected with colchicine, after 20 min of hypotonization. The karyotype was composed of 2n=36 biarmed chromosomes, thirty metacentrics with the rest being submetacentrics, NF=72. In the slides of the gonads the meiotic chromosomes in spermatogenesis were observed as being in prophase I (leptoten, zygoten, and diakinesis) and in telophase I. In diakinesis 18 bivalents were formed. No disturbances were observed during meiosis. The spermatozoa were typical of aquatic molluscs; consisting of a spherical head, a short midpiece and a long tail.
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.