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1

Effect of external addition of rhamnolipids biosurfactant on the modification of gram positive and gram negative bacteria cell surfaces during biodegradation of hydrocarbon fuel contamination

100%
Kaczorek E.
Polish Journal of Environmental Studies
|
2012
|
tom 21
|
nr 4
The bacterial strains from the genera: Bacillus, Pseudomonas, Aeromonas, Achromobacter, and Flavimonas isolated from soil contaminated with crude oil were the subject of studies. The effect of the addition of rhamnolipids on cell surface properties and the removal efficiency of diesel oil were investigated. Rhamnolipids caused the modification of cell surface properties of tested strains, which depended on the amount of external additions of biosurfactant. Additionally, the decrease of Zeta potential was observed after the introduction of rhamnolipids to the diesel oil system. Particle size distribution provides information about system homogeneity and the tendency of particles toward agglomeration. Cell surface hydrophobicity during hydrocarbon biodegradation is a dynamic parameter. There were no different effects, after the addition of rhamnolipids, on the Gram positive and Gram negative bacterial strains. Moreover, the addition of rhamnolipids does not always increase the efficiency of diesel oil biodegradation. The most effective bacterial strain in diesel oil removal was the genus Pseudomonas.
2

Ocena wpływu niejonowego surfaktantu Lutensol GD 70 na biodegradację węglowodorów oleju napędowego przez wybrane szczepy bakteryjne

63%
Smulek W., Kaczorek E.
Woda - Środowisko - Obszary Wiejskie
|
2015
|
tom 15
|
nr 1[49]
3

Modyfikacja właściwości powierzchniowych bakterii glebowych w obecności wybranych preparatów grzybobójczych

51%
Smulek W., Piotrowiak J., Zdarta A., Kaczorek E.
Polish Journal of Agronomy
|
2016
|
nr 26
Intensyfikacja rolnictwa spowodowała wzrost użycia środków grzybobójczych w produkcji roślinnej. Środki ochrony roślin mogą zmieniać lokalne warunki środowiskowe w glebie, do której mogą się dostawać podczas oprysku roślin lub z zaprawami nasiennymi. Wśród wielu badań prowadzonych nad stosowanymi preparatami należy przeanalizować również ich wpływ na mikroflorę glebową. W przedstawionych badaniach przetestowano wpływ wybranych środków grzybobójczych: Acrobat MZ 69 WG (substancje czynne: dimetomorf 90 g•kg-1 i mankozeb 600 g•kg-1) i Gwarant 500 SC (substancja czynna: chlorotalonil 500 g•l-1) na właściwości powierzchniowe mikroorganizmów glebowych Rahnella aquatilis oraz Raoultella planticola. Uzyskane wyniki wykazały, że zarówno Acrobat MZ 69 WG, jak i Gwarant 500 SC modyfikowały powierzchnię komórek szczepów R. aquatilis i R. planticola, zwiększając ich hydrofobowość. Ponadto dodatek Acrobatu MZ 69 WG powodował zwiększenie przepuszczalności wewnętrznej błony komórkowej obu wybranych szczepów glebowych, czego nie zaobserwowano w przypadku preparatu Gwarant 500 SC. Wzrost stężenia obu środków grzybobójczych obniżał wartość potencjału zeta komórek szczepu R. planticola aż do wartości -26,2 mV (w obecności Gwarantu 500 SC), a zwiększał – szczepu R. aquatilis (do -16,9 mV, wobec Acrobatu MZ 69 WG). Uzyskane wyniki wskazują na znaczące modyfikacje powierzchni komórek bakterii glebowych pod wpływem badanych preparatów grzybobójczych. Kierunek oraz intensywność wywołanych zmian zależały od rodzaju oraz dawki stosowanego preparatu. Jednocześnie analiza statystyczna nie wykazała bezpośredniej korelacji między zmianami stężenia a wszystkimi analizowanymi parametrami.
4

Algorytmy diagnostyczno-terapeutyczne w przebiegu bakteryjnych chorób gołębi

51%
Stenzel T., Kaczorek E., Szczucinska E.
Magazyn Weterynaryjny
|
2018
|
tom 27
|
nr 04
5

Wpływ surfaktantów na biodegradację węglowodorów przy udziale bakterii gramdodatnich i gramujemnych

51%
Kaczorek E., Pijanowska A., Olszanowski A.
Biotechnologia
|
2003
|
nr 4
6

The ability of Candida maltosa for hydrocarbon and emulsified hydrocarbon degradation

51%
Chrzanowski L., Kaczorek E., Olszanowski A.
Polish Journal of Environmental Studies
|
2006
|
tom 15
|
nr 1
The yeast strain Candida maltosa EH 15 was used as a biological agent in the hydrocarbon and emulsified hydrocarbon biodegradation. Six different surface active compounds were used to emulsify hydrocarbons. Biodegradation degree and biomass quantity were determined daily over 7 days. The obtained results indicated the strong ability of Candida maltosa EH 15 for hydrocarbon biodegradation. The addition of the tested surfactants influenced hydrocarbon biodegradation; however, biodegradation effectiveness varies with the type and concentration of surfactant.
7

Analysis of surface tension during biodegradation of hydrocarbons

51%
Kaczorek E., Olszanowski A., Cybulski Z.
Polish Journal of Environmental Studies
|
2005
|
tom 14
|
nr 2
Biodegradation of a model mixture of hydrocarbon (dodecane and hexadecane, 1:1 w/w), as well as hydrocarbon emulsified by synthetic and natural emulsifiers, and their mixtures were analyzed in the presence of Pseudomonas spp. and Bacillus spp. bacterial strains. Changes in surface tension were measured by the static and dynamic methods during biodegradation processes. During the biodegradation processes of hydrocarbon and emulsified hydrocarbon, various changes in surface tension were observed. In some systems, the initial decrease of surface tension after the addition of an emulsifier was greater after a few days of biodegradation. Decrease in the surface tension does not seem to be related to the degree of hydrocarbon biodegradation, both for the hydrocarbon and emulsified hydrocarbon systems.
8

Examination of biodegradation of hydrocarbons in emulsified systems

51%
Bielicka K., Kaczorek E., Olszanowski A., Voelkel A.
Polish Journal of Environmental Studies
|
2002
|
tom 11
|
nr 1
This paper presents the biodegradation process carried out in water samples. Aliphatic hydrocarbons were degraded in the presence of the emulsifier. Non-ionic surfactant was used as the emulsifier. Two bacteria strains from the Pseudononaceae family were used in this process. We used Solid-Phase Extraction and gas chromatography for qualitative and quantitative determination of the components of the biodegradation process.
9
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The in vitro effect of kynurenic acid on the rainbow trout (Oncorhynchus mykiss) leukocyte and splenocyte activity

45%
Malaczewska J., Siwicki A. K., Wojcik R., Turski W. A., Kaczorek E.
Polish Journal of Veterinary Sciences
|
2014
|
tom 17
|
nr 3
Kynurenic acid (KYNA), an endogenous neuroprotectant formed along the kynurenine pathway of tryptophan degradation, is a selective ligand of the GPR35 receptor, which can be found on the surface of various populations of human immune cells. In infections and inflammations, KYNA produces an anti-inflammatory effect through this receptor, by depressing the synthesis of reactive oxygen species and pro-inflammatory cytokines. However, it is still unrecognized whether receptors for kynurenic acid are also localized on immune cells of poikilothermic animals, or whether KYNA is able to affect these cells. The objective of this study has been to determine the effect of different concentrations of kynurenic acid (12.5 μM to 10 mM) on the viability and mitogenic response of lymphocytes and on the activity of phagocytic cells isolated from blood and the spleen of rainbow trout. The results imply low toxicity of kynurenic acid towards fish immune cells, and the proliferative effect observed at the two lowest concentrations of KYNA (12.5 μM and 25 μM) seems indicative of endogenous kynurenic acid being capable of activating fish lymphocytes. Non-toxic, micromole concentrations of KYNA, however, had no influence on the mitogenic response of lymphocytes nor on the activity of phagocytes in rainbow trout under in vitro conditions. There is some likelihood that such an effect could be observed at lower, nanomole concentrations of KYNA.
10
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Evaluation of Lactobacillus spp. and yeast based probiotic (Lavipan) supplementation for the reduction of Salmonella Enteritidis after infection of broiler chickens

39%
Smialek M., Kaczorek E., Szczucinska E., Burchardt S., Kowalczyk J., Tykalowski B., Koncicki A.
Polish Journal of Veterinary Sciences
|
2019
|
tom 22
|
nr 1
11

Wpływ preparatu mikrobiologicznego EM-Probiotyk na odporność nieswoistą oraz odporność przeciwzakaźną pstrąga tęczowego

39%
Terech-Majewska E., Grudniewska J., Duchiewicz K., Pajdak J., Kaczorek E., Schulz P., Siwicki A. K.
Komunikaty Rybackie
|
2016
|
nr 2
12

Zastosowanie bakteriofagów w ukierunkowanej terapii chorób bakteryjnych ryb - badania doświadczalne

33%
Siwicki A. K., Dastych J., Wojcik E., Schulz P., Kaczorek E., Kazun K., Kazun B., Lepa A., Terech-Majewska E.
Komunikaty Rybackie
|
2014
|
nr 5
The increasing resistance of bacteria to available antibiotics constitutes one of the greatest challenges in intensive fish culture. The growing problem of chemotherapeutic resistance has revived interest in the potential use of bacterial viruses (bacteriophages) for therapy of bacterial diseases in fish. Bacteriophages are viruses specific for bacterial strains and are natural regulators of bacterial populations in waters. In the present study, we determined the influence of specific bacteriophages on mortality percentages after experimental challenges with the pathogenic bacteria Aeromonas hydrophila in rainbow trout. Healthy rainbow trout, weighing 50-100 g were used in the study. Bafador-1 with 1x108 PFU/ml or 1x109 PFU/ml concentrations of bacteriophages were used in immersion or injection at 12,24, or 48 h after experimental infections. The results showed that Bafador-1 reduced mortality after application in immersion or injection at a concentration of 1 LVm3 of water, or 0.2 ml per fish. The three experimental studies presented indicated that Bafador-1 is very effective in reducing mortality after experimental infection with the pathogenic bacteria A. hydrophila. The preliminary study showed that specific bacteriophages are very effective in specific therapy of bacterial diseases in fish.
13

Wpływ efektywnych mikroorganizmów na nieswoistą odporność komórkową u sandacza

33%
Terech-Majewska E., Kaczorek E., Schulz P., Zakes Z., Rozynski M., Kowalska A., Zembrzuska M., Siwicki A. K.
Medycyna Weterynaryjna
|
2018
|
tom 74
|
nr 05
Effective Microorganisms (EM) technology uses microorganisms to work in the surrounding environment. Complex microbiological preparations show synergistic effects, combining the effects of probiotics, prebiotics and synbiotics. EMTM technology is well-known and used in 120 countries worldwide, mainly in agriculture, including aquaculture, and in environmental protection. Fish farmers use these preparations to enhance fish growth, condition and immunity. The aim of the study was to evaluate the effect of EM-Probiotic (Greenland) on the development of cellular non-specific resistance in pikeperch (Sander lucioperca) at the initial stage of rearing in a recirculating aquaculture system (RAS). The experimental diet was administered for 28 days with EM supplementation at 0% (control group), 2% and 4% of the feed. Water temperature in the RAS was maintained at 22.0ºC. Hematopoietic organs (spleen and kidney) were collected twice, after 28 days of EM supplementation (first term) and 28 days after cessation of supplementation (second term). The respiratory burst activity (RBA) and potential killing activity (PKA) of macrophages, as well as the proliferative activity of T- and B-lymphocytes (MTT assay) were assessed. The results of the study show that EM at the initial stage of pikeperch development (body weight of 2 to 14 g) suppress cellular defence mechanisms, decreasing the activity of immunocompetent cells in RBA, PKA and MTT tests. The results from the first organ harvesting showed differentiated EM activity at a statistically insignificant level (P > 0.05). In samples collected 28 days later, the inhibitory effect was demonstrated at a statistically significant level (P < 0.05) in all parameters in both experimental groups. Non-specific cellular immunity in fish plays a key role in defence against damaging factors, including pathogenic ones. Since fish in an RAS are also susceptible to potentially pathogenic agents, immunosuppression of these mechanisms may aggravate the disease.
14

Monitorowanie i ocena stanu zdrowia węgorza europejskiego Anguilla anguilla (L.) w Polsce

33%
Robak S., Siwicki A. K., Kazun B., Kazun K., Lepa A., Schulz P., Kaczorek E., Szczucinska E., Terech-Majewska E.
Komunikaty Rybackie
|
2014
|
nr 6
The significance of eel to fisheries management and the environment necessitates the continuous monitoring of the health and condition of this valuable species. One of the many monitoring studies realized within the project was to determine the condition and health status of eel used for stocking open waters and those inhabiting various basins in Poland. The newest research and diagnostic methods were used to conduct these targeted studies. The studies were performed by a research team comprised of staff from the Department of Ichthyology at IFI, the Department of Fish Pathology and Immunology at IFI, the Department of Microbiology and Clinical Immunology and the Department of Epizootilogy in the Faculty of Veterinary Medicine at UW-M in Olsztyn, and the Department of Food and Environmental Chemistry at the National Marine Fisheries Research Institute in Gdynia. Full clinical, anatomic pathological, parasitological, mycological, virological, bacteriological, biochemical, and immunological studies were performed. The analysis of the condition and health status of stocking material indicated that eel originating from Polish and foreign hatcheries for stocking open waters did not resent and pathology that would indicate and acute or chronic diseases. Neither the EVEX or the AngHV-1 viruses were isolated from the fish analyzed, nor was the presence of the VHS, IHN, IPN, or SVC viruses confirmed. The analysis of the condition and health of eel inhabiting open waters indicated that approximately 20% of the fish from each basin exhibited abrasions, discoloration, small skin lesions, and bruising on the caudal and pelvic fins. Only in single specimens from some basins was pathology indicative of disease confirmed. The bacteria Aeromonas hydrophila, A. sobria and Flavobacterium psychrophilum were isolated from them. Targeted virological studies of one eel specimens from the Szczecin Lagoon confirmed the occurrence of the AngHV-1 virus. An important element of the research was that among the fish monitored from various basins a systematic increase in the prevalence of the nematode Anguillicoloides crassus was noted at an intensity and extensiveness of invasion that ranged from 50 to 100% of the studied fish population
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