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As immunosuppression in pigeons is common and results in reduced post-vaccination immunity and lower health status of the birds, studies have been taken up aimed at evaluation of the effect of three doses of methisoprinol on the percentage of CD4+ and CD8+ T lymphocyte subpopulation in peripheral blood and in the spleen and the titre of anti-NDV antibodies in the serum of pigeons in four groups (A, B, C, D), with 20 birds each. Pigeons in each group were immunised against paramyxovirosis at week 6 and 9 of life. Water for injection (group A – control) or methisoprinol at 100 mg/ kg of body weight (group B), 200 mg/kg of body weight (group C) and 600 mg/kg of body weight (group D) was administered intramuscularly for 3 days before each vaccination. The immunological analyses were carried out by flow cytometry and the ELISA test. The findings indicate that methisoprinol administered intramuscularly at 100 and 200 mg/kg of body weight for 3 successive days before vaccination against paramyxovirosis mainly stimulates the mechanisms of non-specific humoral and cellular immunity, which is indicated by a higher percentage of the subpopulation of CD4+ T lymphocytes in peripheral blood and in the spleen and a higher titre of anti-NDV antibodies.
As a consequence of frequent immunosuppression in pigeons, with resultant decreased post-vaccination immunity and deteriorated health of the birds, a study was taken up in order to determine the effect of three immunomodulators (β-glucans, metisoprinol and levamisole) on the percentage of CD4⁺ and CD8⁺ T lymphocytes in peripheral blood and spleen, and the titre of anti-NDV antibodies in the serum of pigeons in four groups (A, B, C, D), 20 birds in each. The birds in each group were immunized against paramyxovirosis in week 6 and 9 of their lives, and water for injection was given intramuscularly 1 day before each injection (group A control), metisoprinol was given intramuscularly for 3 days at a dose of 300 mg/kg of body weight (group B), levamisole was given as a 7.5% solution of levamisole hydrochloride at a dose of 2 mg/kg of body weight intramuscularly, 1 day (group C), or β-glucans were given 10 days before vaccination per os at a dose of 5 mg/kg of body weight (group D). The immunological examinations were carried out by flow cytometry and the ELISA test. The results indicate that levamisole and β-glucans at the doses used in the study stimulate an increase in the anti-NDV antibody titre and the percentage of CD4⁺ T lymphocyte subpopulation in peripheral blood (levamisole) along with an increase in the percentage of CD8⁺ T lymphocytes in the spleen of pigeons vaccinated against paramyxovirosis. The absence of such an effect following the administration of metisoprinol at a dose of 300 mg/kg of body weight for 3 days may have resulted from an excessively high dose.
The aim of the study was to determine the level of air bacteria and fungi in the horse stable and the emission of microorganisms to the external environment during different seasons of the year. The predominating fungi in the stable and outside were also identified. These results demonstrate that both in the stables and outdoors, the number of microorganisms was the highest in the summer. This tendency was especially noticeable in the case of the concentrations of fungi outside the building, when the average value was significantly higher (P<0.01) than in other seasons. No statistically significant differences in the concentrations of airborne microorganisms at different distances from the stable were observed. Penicillium sp., Fusarium sp., and yeast predominated inside and outside the stable.
The aim of this study was to compare the breeding environmental conditions in the box stall stable and the horse-barn. Assessment of the breeding environment was performed on the basis of the stable area and the cubic capacity as well as on microclimate measurements over the autumn and winter period. The results of the study indicate that both the box stall stable and the horse-barn provided a proper areal and cubic conditions, but the microclimate conditions were better in the stall box stable. In the horse-barn most of microclimate parameters (air temperature, relative humidity, air movement, cooling power and natural illumination conditions) exceeded the allowable level in larger degree than in the box stall stable. The dust pollution level in both stables, did not exceeded the allowable level and its size dependent on work conducted in the stables.
The aim of this study was to assess the relationship between living conditions (microclimate, aerial contaminations, box dimension, access to the paddock/pasture) of horses kept in 3 stables and physiological indicators (protein and its fractions in serum, haptoglobin). The study was carried out for 4 months during autumn and winter in 3 stables. Blood samples were collected from 10 clinically healthy adult horses from each stable (30 in total) in 4 terms – every 4 weeks. Environmental conditions were evaluated according to zoohygiene methodology and current law regulation. In the blood serum, the analyzed parameters were: total protein (g/L) – with the biuret test, the contribution of individual serum protein fractions (%) (albumin and α1-, α2-, β- and γ-globulin) – set to serum protein electrophoresis on agarose gels in an SAS-MX electrophoresis chamber; haptoglobin concentration (g/L) – immunoturbidimetric test. Living conditions meeting the requirements of welfare were provided in stable 3 in terms of both microclimatic parameters as well as the dimensions of the boxes and access to paddock and pasture. Slightly worse conditions were recorded in stable 2, while stable 1 was characterized by not only the worst microclimatic parameters, but also the surface of the boxes was too small. The different living conditions of horses in different stables were reflected in the diverse values of the blood indicators. Although the obtained ratios of protein indicators corresponded to the values of normal adult healthy horses, it was demonstrated that in the serum of horses in stables 1 and 2, as compared to the stable 3, a significantly lower fraction of albumin and higher α2-globulin and a higher concentration of Hp was found – suggesting the effect of different living conditions on organism homeostasis indicators. It may be stated that living conditions significantly influence the physiological condition of the horse.
The aim of this study was to determine the effect of different variants of pre-slaughter procedures in the winter on body weight loss in broiler chickens, and on the proximate chemical composition and physicochemical properties of meat. At the completion of a six-week rearing period, 240 ROSS 308 chickens were subjected to the following variants of pre-slaughter transport: no transport (N-T), transport to a distance of 100 km (T-100), 200 km (T-200) and 300 km (T-300). The acidity of breast muscles (pH₁₅ and pH₂₄) was measured with a portable meat pH-meter, HI 99163 (Hanna Instruments, Germany) supplied with an FC 232D electrode. Meat color (15 minutes and 24 hours post mortem) was determined with the use of a Minolta CR-400 chroma meter (Japan), in the CIE L* a* b* system. Water-holding capacity (24 hours post mortem) was determined as drip loss. The content of water, protein and fat in meat was determined using an InfraLab 710 near-infrared analyzer (NDC Infrared Engineering, UK). The results of the conducted study showed that the elongation of transport length for a distance of 200 km and 300 km influenced the increase of broiler weight loss. Significantly higher (P = 0.01) weight loss was observed after transport to a distance of 200 and 300 km, compared to a group of chickens from group T-100 (respectively 2.65%, 2.36% and 1.41%). It cannot be stated clearly that variants of transportation have contributed to the deterioration of the quality of the meat acquired. The longest transport distance resulted in a faster rate of the pectoral muscle glycolysis in group T-300 (0.69 units) compared to the other groups (0.60-0.62 units). The broiler meat from group T-300, as compared to the other groups, was also characterized by a significantly higher surface of drip loss and the parameters L * 24 and b * 24, which reflected in its significantly lighter color and lower water-holding capacity. However, the final pH values were similar in all groups and did not show any meat defects. In addition, the basic chemical composition of chicken muscles showed their good nutritional value.
Celem przeprowadzonych badań było określenie zawartości: Mg, Ca, Na, K, P, Fe w mięśniach piersiowych kurcząt brojlerów ROSS 308, poddanych w okresie lata 2010 r. różnym wariantom postępowania przedubojowego. W postępowaniu przedubojowym podawano kurczętom w paszy lub w wodzie, przez siedem dni przed ubojem, fitopreparat składający się z: rutwicy lekarskiej (Herb. Galegae), pokrzywy zwyczajnej (Herb. Utricae), melisy lekarskiej (Fol. Melissae) i szałwii lekarskiej (Fol. Salviae), a następnie transportu kurcząt na różne odległości: 100, 200 oraz 300 km. Stwierdzono, że postępowanie przedubojowe wpłynęło na zawartość składników mineralnych w mięśniach piersiowych kurcząt brojlerów. Podawanie kurczętom przez siedem dni przed planowanym obrotem przedubojowym fitopreparatu wraz z paszą wpłynęło nieznacznie na wzrost zawartości składników mineralnych w mięśniach piersiowych w porównaniu z grupą kontrolną i grupą otrzymującą fitopreparat z wodą do picia. Wykazano zbliżony poziom lub zwiększenie zawartości pierwiastków w mięśniach piersiowych kurcząt transportowanych na odległość 100 i 200 km. Natomiast w mięśniach kurcząt transportowanych na odległość 300 km nastąpiło zmniejszenie zawartości pierwiastków w stosunku do grupy nietransportowanej.
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