We analysed the essential oils of cultivated material of Thymus xtoletanus Ladero and its parents, Th. mastichina (L.) L. and Th. villosus subsp. lusitanicus (Boiss.) Coutinho, with seeds from the same locality in the centre of the Iberian Peninsula. The essential oil of Th. xtoletanus, which had not been analyzed previously, presented 1,8-cineole as the major component (25.5%), as was also the case for Th. mastichina (76.1%). Other components with a major presence in Th. xtoletanus and Th. villosus subsp. lusitanicus were [(Z)-β-ocimene (8.1%); camphor (4.5%); sabinene (3.2%); α-pinene (2.8%)], and [(Z)-β-ocimene (4.1%), camphor (9.8%), sabinene (2.8%), α-pinene (7.1%)], respectively. However, in the hybrid the components β-phelandrene (14.5%), limonene (6.9%), (E)-γ-bisabolene (3.5%), and viridiflorol (3.3%) stood out, inter alia, whereas their presence in the parents was limited. The study confirms the hybrid origin of Th. xtoletanus as intermediate between Th. mastichina and Th. villosus subsp. lusitanicus.
The essential oil of a protected Spanish species, Thymus praecox ssp. penyalarensis, was firstly analyzed [Gas Chromatography-Mass Spectrometry (GC-MS)] from wild populations during the phenological stages of flowering and fruiting. In flowering, the yield was 1.2%, and the major components were identified as thymol (18.5%), p-cymene (14.6%), carvacrol (11.6%), and γ-terpinene (10.1%). In fruiting, the yield was 0.9% and the major components resulted to be p-cymene (19.0%), carvacrol (16.5%), and borneol (10.5%). An experimental cultivation of the taxa was made also in order to analyze its essential oil as well. The yield was 1.3%, and the main compounds were thymol (19.1%), p-cymene (17.0%), and γ-terpinene (12.3%). Finally, the composition of the essential oil of this plant is discussed in relation to chemotaxonomic and biogeographical aspects.
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