An analysis of Odonata check-lists of protected species and territories of national and international significance have been carried out within the framework of a biodiversity data base of Ukrainian National Ecological Networks key areas. Summarised and updated information on 11 protected areas is reported. For five natural reserves, such as Ielanetskyi steppe, Kazantyp, Cheremskyi and Karadagskyi Nature Reserves and one branch of Ukrainian Steppe Reserve the check-lists of dragonflies are presented for the first time.