Wyniki wyszukiwania

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Protective effect of inosine against oxidative-stress induced DNA damage

100%

Kalinska-Blach B., Gil A., Halon H., Wozniak M.

Acta Biochimica Polonica

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2003

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tom 50

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nr Supplement 1

2

Selectivity of oxidative stress targeting in estrogen-induced experimental nephrocarcinogenesis

100%

Kobiela J., Krajewski J., Kalinska-Blach B., Stefaniak T.

Acta Biochimica Polonica

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2002

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tom 49

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nr 1

Specificity of targeting of the oxidative stress towards lipid and protein fractions in a model of estrogen-induced Syrian hamster nephrocarcinogenesis was evaluated. The amount of proteins modified by oxidative stress was significantly elevated as early as one month after the initial implantation of estradiol to the experimental versus the control group, while the stress did not affect lipids. Subcellular localization of the oxidative stress target was determined by the analysis of protein oxidation in subcellular fractions of kidney cells. The endoplasmic reticulum membranes were the fraction most affected by the oxidative stress.

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Protein oxidation products and plasma antioxidants in juvenile idiopathic arthritis

76%

Renke J., Popadiuk S., Korzon M., Kalinska-Blach B., Szumera M., Wozniak M.

Acta Biochimica Polonica

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2003

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tom 50

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nr Supplement 1

4

Dynamics of estrogen-induced oxidative stress

51%

Kobiela J., Stefaniak T., Krajewski J., Kalinska-Blach B., Zurawa-Janicka D., Lachinski A., Gackowski D., Olinski R., Nowak J., Knap N., Lipinska B., Sledzinski Z., Wozniak M.

Acta Biochimica Polonica

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2007

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tom 54

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nr 2

The objective of this study was to assess the dynamics of oxidative damage to cellular macromolecules such as proteins, lipids and DNA under conditions of oxidative stress triggering early stages of estrogen-dependent carcinogenesis. A rodent model of carcinogenesis was used. Syrian hamsters were sacrificed after 1, 3, 5 h and one month from the initial implantation of estradiol. Matching control groups were used. Kidneys as target organs for estradiol-mediated oxidative stress were excised and homogenized for biochemical assays. Subcellular fractions were isolated. Carbonyl groups (as a marker of protein oxidation) and lipid hydroxyperoxides were assessed. DNA was isolated and 8-oxodGuo was assessed. Electron paramagnetic resonance spectroscopy was used to confirm the results for lipid peroxidation. Exposition to estradiol in the rodent model leads to damage of macromolecules of the cell, including proteins and DNA, but not lipids. Proteins appear to be the primary target of the damage but are closely followed by DNA. It has previously been speculated that protein peroxides can increase DNA modifications. This time sequence was observed in our study. Nevertheless, the direct relation between protein and DNA damage still remains unsolved.

Ograniczanie wyników

4 Acta Biochimica Polonica

4 Kalinska-Blach B.

3 Wozniak M.

2 Kobiela J.

2 Krajewski J.

2 Stefaniak T.

1 Gackowski D.

1 Gil A.

1 Halon H.

1 Knap N.

1 Korzon M.

1 Lachinski A.

1 Lipinska B.

1 Nowak J.

1 Olinski R.

1 Popadiuk S.

1 Renke J.

1 Sledzinski Z.

1 Szumera M.

1 Zurawa-Janicka D.

1 2007

2 2003

1 2002

Protective effect of inosine against oxidative-stress induced DNA damage

Selectivity of oxidative stress targeting in estrogen-induced experimental nephrocarcinogenesis

Protein oxidation products and plasma antioxidants in juvenile idiopathic arthritis

Dynamics of estrogen-induced oxidative stress