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Production of intergeneric allotetraploid between autotetraploid non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino) and autotetraploid radish (Raphanus sativus L.)

100%
Sun C.-Z., Li Y., Zhang S.-N., Zheng J.-S.
Acta Societatis Botanicorum Poloniae
|
2014
|
tom 83
|
nr 1
Intergeneric hybrids between non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino; 2n = 4x = 40) and radish (Raphanus sativus L.; 2n = 4x = 36) were obtained through ovary culture and embryo rescue. Some hybrid embryos (0.11 per ovary) were produced, but only 4 of them germinated. As most hybrid embryos failed to develop into plantlets directly, plants were regenerated by inducing shoots on the cultured cotyledon and inducing roots on the root induction medium. All hybrid plants were morphologically uniform. They resembled the non-heading Chinese cabbage in the long-lived habit, the plant status, the vernalization requirement and the petiole color, while the petiole shape, leaf venation pattern and flowers were more similar to those of radish. Upon examination of the flowers, these were found to have normal pistil, but rudimentary anthers with non-functional pollen grains. The somatic chromosome number of F1 plants was 38. Analysis of SSR banding patterns provided additional confirmation of hybridity.
2

Therapeutic effects of revascularisation on the healing of free bone grafts in dogs

76%
Zheng J.-S., Ruan H.-R., Qiu S., Nie J., Hou K.-W., Wu R.
Journal of Veterinary Research
|
2020
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tom 64
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nr 1
3

Serum metabolomic analysis of feline mammary carcinomas based on LC-MS and MRM techniques

76%
Zheng J.-S., Wei R.-Y., Wang Z., Song J., Ge Y.-S., Wu R.
Journal of Veterinary Research
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2020
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tom 64
|
nr 4
4

Screening of early diagnostic markers of gentamicin-induced acute kidney injury in canines

76%
Zheng J.-S., Nie J., Zhu T.-T., Ruan H.-R., Wei X., Wu R.
Journal of Veterinary Research
|
2019
|
tom 63
|
nr 3
5

Characterization of a glutamine synthetase gene BnGS1-2 from ramie (Boehmeria nivea L. Gaud) and biochemical assays of BnGS1-2-over-expressing transgenic tobacco

76%
Zheng J.-S., Yu C.-M., Chen P., Wang Y.-Z., Tan L.-T., Chen J.-K., Xiong H.-P.
Acta Physiologiae Plantarum
|
2015
|
tom 37
|
nr 01
Glutamine synthetase (GS) plays fundamental roles in nitrogen metabolism in higher plants. Ramie (Boehmeria nivea L. Gaud) has excellent forage and rapid growth characteristics, and isolated BnGS1-2 is closely related to that of legumes, potentially providing gene resources for nitrogen uptake and assimilation. Consequentially, transgenic studies involving the over-expression of BnGS1-2 in Nicotiana tabacum were performed to investigate the potential function and application of BnGS1-2 in higher plants. Comparative analysis of nitrogen use efficiency (NUE) was performed during wild type and independent transgenic lines with various in GS activity at the mRNA and protein levels. Our results indicate that the BnGS1-2-over-expressing transgenic lines significantly enhanced the fresh weight, dry weight and plant height. Simultaneously, the transgenic lines had higher soluble protein content and higher total nitrogen content than the control plants under normal planting condition. The range of enhancement positively associated with BnGS1-2 activities in different transgenic lines. The over-expression of BnGS1-2 in tobacco resulted in a significant decrease in leaf-free NH4+ content. Simultaneously, the transgenic lines increased the NO3- uptake and NH4+ assimilation with a remarkable increase in the NR activity and no change in the free NO3- content. These results indicate that BnGS1-2would be an excellent gene resource for improvement of plants biomass and NUE.
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