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The aim of this study was to evaluate the microbiological quality, pH values, and sensory parameters of swine meat in order to determine its status depending on lung pathologies established after slaughter. Standard bacteriological techniques were used for microbiological analysis. Total aerobe count during the periods of the experiment increased in all investigated swine groups having different degrees of lung lesions. The study results showed that marked lung alterations influence the microbial quality of pork. The total aerobe counts in swine meat with a medial degree of pneumonia had no influence on the microbiological quality of the meat. It was demonstrated that the meat pH of healthy pigs was lower whereas the mean value of pH of pigs with various degrees of lung lesions was higher. The meat pH of swine with lungs highly affected by pneumonia showed the highest values, which indicated that pH changes depend on lung affections that suggest it technological quality. Lung pathologies influenced meat sensory parameters as well. Swine meat highly affected by lung lesions putrefied at the 6th d after slaughter.
The amounts of biogenic amines (putrescine, histamine, cadaverine, tyramine, spermidine, and spermine), lactic acid, pH, and number of Enterobacteriaceae, Escherichia coli, and coagulase producing Staphylococci were determined in healthy turkeys and in the fillets of turkey breasts with the signs of ascites and bursitis. The examination of the amines was performed by high performance liquid chromatography; the amount of lactic acid was determined by capillary isotachophoresis method; pH value and microbiological indicators - by standardised methods. All determinations were done after 24, 72, and 120 h after slaughter of the turkeys. The fillets of the breasts were stored in the refrigerator at 4°C. The significant differences in the total amounts of biogenic amines in carcasses of healthy turkeys and carcasses of turkeys with substantial lesions of bursitis and medium or substantial lesions of ascites were determined within 5 d after slaughter (P<0.00l). The amounts of biogenic amines in the breast fillets of turkeys with low lesions of ascites and bursitis were very similar to that of the control group during the whole period of maturation. The average amount of lactic acid throughout 5 d after slaughter increased only in the breast fillets of healthy turkey (maximum value was 1.032 mg/100 g) and insignificantly increased in the carcasses with low lesions of ascites and bursitis (maximum value was 0.983 mg/100 g). The results of investigation indicate the breast fillets of turkeys with medium and substantial lesions of ascites and substantial lesions of bursitis are not fit for human consumption. However, in the cases of low lesions of ascites and low and medium lesions of bursitis, the fillets are not fit for maturation, but could be thermally processed in the period of 24 h after slaughter.
Forty-five cattle of different ages and gender were selected from three separate farms with a total number of 929 animals. Blood serum samples from each of the animals were tested twice at two-month intervals for bovine viral diarrhoea virus (BVDV) antigen (BVDV Ag) and BVDV antibodies (BVDV Ab) using ELISA. Five animals were found to be BVDV Ag positive and BVDV Ab negative. Therefore, their blood and saliva samples were subjected to further investigation. The samples of blood serum and saliva were additionally screened by a nested reverse transcription PCR (RT-nPCR), real-time PCR, and virus isolation to confirm BVDV persistent infection. Viral RNA was isolated from blood and saliva samples. The cDNA was synthesised and amplification of DNA was performed. The results of RT-nPCR were analysed by gel electrophoresis using ethidium bromide while those of real-time PCR were interpreted according to the amplification curve. Laboratory testing of blood and saliva samples revealed 5 persistently infected (PI) animals from one farm with 579 cattle (0.9% prevalence). The results were confirmed by RT-nPCR and real-time PCR screening samples of blood serum. Using PCR techniques and virus isolation, BVDV RNA was detected; however, the level of viral RNA in saliva was found to be lower than that in blood serum. The results obtained show the possibility to identify PI animals by RT-nPCR and real-time PCR techniques from saliva samples. The collection and testing of saliva is a simple and quick technique, and can be successfully applied in field conditions to identify PI animals, avoiding the risk of intervention while sampling blood or dependence on animal gender and lactation period while sampling milk or semen.
A serological study of BoHV-1 distribution was conducted in Lithuania from 2005 to 2009. Antibody level was measured using a commercial ELISA. For serological examination, 15,368 random blood samples from cattle of different age, gender, and size of herd, which was unvaccinated against IBR, were collected in 37 districts. It was registered that 11.97% of BoHV-1 were seropositive samples. It was also shown that BOHV-1 is most widespread in cattle herds with population >200 individuals (14.79%). Comparison of different sex groups of cattle revealed that the highest number of infected animals was identified in cows (34.64%) and the lowest in bulls (2.01%). In heifers the number of infected animals was 10.01% and in calves - 4.41%. It was shown that seroprevalence of BoHV-1 infection in Lithuania increased with age of animals. The highest prevalence of BoHV-1 (53.98%) was registered in cattle aged more than 7 years.
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