Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 5

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
Salicornia bigelovii is an unusually salt-tolerant plant species and is able to continue to photosynthesize and grow in saline conditions. There is an interest in understanding the mechanisms of this salt tolerance. In this study, S. bigelovii plants grown under 400 mM NaCl had higher growth and higher photosynthetic capacity than those not grown with salt. The intensities of the fluorescence emission peaks in the thylakoid membrane spectra in the salt treatment were higher than in the control. PSII and PSI peaks of the control were blue-shifted, indicating that salt deficiency affects energy transfer and structural stability in PSII and PSI. Both SDS-PAGE and western blot illustrated that the PSI proteins PsaA and PsaB and the PSII proteins CP47 and CP43 were upregulated with salt treatment, which might partly explain the change in the energy transfer process. Furthermore, Lhcb1 was also stimulated by salt treatment while Lhcb2 and Lhcb3 did not show noticeable changes. This demonstrated that salt is important in the maintenance of photosynthesis. In summary, treatment with salt led to an increased amount of PsaA/B, CP47, CP43, and Lhcb1 with a concurrent increase in antennae size. These photosystem changes may be responsible for the adaptation of S. bigelovii to saline conditions.
A subtractive cDNA library was developed to study genes associated with the release of dormant buds in tree peony. To identify genes that are highly expressed in buds released from dormancy, 588 clones were examined by differential screening and then 185 clones were selected to be sequenced. A total of 31 unique genes were obtained, of which only 25 sequences had matches in the NCBI database or Arabidopsis thaliana protein database while 6 sequences with no matches. Many of the different genes were identified as having unknown or hypothetical functions while others were speculated to have different molecular functions. The expression profiles of the selected ‘‘candidate’’ genes which may be associated with dormancy release according to their putative function and previous reports were assessed by northern blot and semiquantitative RT-PCR. The results indicated that the transcriptional expressions of the isolated genes are related to growth regulation and stress response. Our results provide interesting information for further understanding the molecular mechanism of bud dormancy release in tree peony.
Background: Epstein–Barr virus (EBV) infection is causatively associated with a variety of human cancers, including gastric cancer (GC), which has one of the highest mortality rates of all human cancers. Long non-coding RNAs (lncRNAs) show important regulatory roles in human GC. SNHG8 is a recently identified lncRNA that was reported to show abnormal expression pattern in GC. However, little is known of its biological function in EBV-associated GC. Methods: We used cell viability, colony formation and cell cycle assays to investigate the roles of lncRNA SNHG8 in the cell growth of EBV-associated GC. Results: The transcript levels of SNHG8 in the cultured EBV-associated GC cells were significantly higher in the cultured EBV-associated GC cells compared with the levels in normal human gastric mucosal cells and EBV-negative GC cells. Knockdown of SNHG8 with specific shRNAs inhibited cell proliferation and colony formation and arrested the cell cycle in the G0/G1 phase in vitro. We also found that knockdown of SNHG8 suppressed tumor growth in vivo. Conclusions: These data indicate the pro-oncogenic potential of SNHG8 in EBVassociated GC, meaning it is a latent therapeutic target for the treatment of this type of cancer
Mitochondrial uncoupling proteins (UCPs) play crucial role in the regulation of metabolic thermogenesis, photosynthesis, redox poise, and response to stress situations. However, the involvements UCPs and its regulatory mechanisms in plant mitochondrial respiration and electron transport have not yet been fully substantiated. In this article, we show that UCP is essential for effective respiratory balance and mitochondrial electron transport poise under chilling stress in tomato. Compared with pTRV (non-silenced) plants, suppression of LeUCP the expression by virus-induced gene silencing significantly decreased the total, SHAM-resistant, and CN-resistant respiratory rates, but increased cytosolic glycolysis pathway- related gene expression and enzymes’ activities under chilling stress. The transcripts’ abundances of UCRH, COX1, COX2, AOX1a, AOX2, and NDC1 genes involved in main and bypass miETC components were decreased, while those of NDUO and SDH4 were slightly increased in the leaves of LeUCP-silenced plants. In addition, silencing of LeUCP aggravated H2O2 and O2- accumulation levels in leaves under chilling stress. Our results provide strong evidence that LeUCP is critical for respiratory homeostasis and mitochondrial electron transport signaling under chilling stress in tomato.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.