Effect of Ascaris trypsin inhibitor on fetal development of mice

• Autorzy: Blaszkowska J
• Języki publikacji: EN

Abstrakty

EN

In this study, maternal toxicity and developmental effects of exposure to Ascaris trypsin inhibitor were evaluated in mice. Pregnant BALB/c females were injected intraperitoneally by Ascaris inhibitor /AIT/ at 200, 300, and 400 mg/kg body weight/day, on days 12 to 15 of gestation (stage of fetal development). At day 19 of pregnancy, uterine contents were inspected for implantation sites, early resorptions (moles), living fetuses and dead fetuses. The living fetuses were weighed and examined for external, internal and skeletal abnormalities. The results showed that AIT induced maternal toxicity, evidenced by maternal deaths, abortions, bleeding from uterus and reduced body weight gain as compared to control (p <0.01). There were no differences between the control group and the rest of all groups investigated for total implantation sites and early resorptions. Fetotoxicity was observed as shown by the decrease in the number of living fetuses and mean fetal weight, a high rate of intrauterine fetal deaths, delayed skeletal ossification, occurrence of pathological changes of fetal organs and tissues. Only one type of congenital malformations (hydronephrosis) was noted in fetuses after injection of higher doses of AIT.

Słowa kluczowe

EN

mouse; mice; trypsin inhibitor; fetal development; Ascaris suum

• Wydawca: -
• Czasopismo: Wiadomości Parazytologiczne
• Rocznik: 2005
• Tom: 51
• Numer: 2
• Opis fizyczny: p.133-138,fig.,ref.

Twórcy

autor

Blaszkowska J

• Medical University, Pl.Hallera 1, 90-647 Lodz, Poland

Bibliografia

• [1] O'Lorcain P., Holland C.V., 2000. The public health importance of Ascaris lumbricoides. Parasitology 12: 51-71.
• [2] Anderson T.J. 1995. Ascaris infection in humans from North America: molecular evidence for cross-infection. Parasitology 110: 215-219.
• [3] Olson M.E., Guselle N. 2000. Are pig parasites a-human health risk? Advances in Park Production 11:.159-162.
• [4] Hayashi K., Tahara H., Yamashita K., Kuroki K.,.Matsushita R., Yamamoto S., Hori T., Hirono S.,.Nawa Y., Tsubouchi H. 1999. Hepatic imaging studies on patients with visceral larva migrans due to probable Ascaris suum infection. Abdominal Imaging 24: 465-469.
• [5] Inatomi Y., Murakami T., Tokunaga M., Ishiwata K., Nawa Y., Uchino M. 1999. Encephalopathy caused by visceral larva migrans due to Ascaris suum. Journal of Neurology Sciences 164: 195-199.
• [6] Villar J., Klebanoff M., Kestler E. 1989. The effects on fetal growth of protozoan and helminth infection during pregnancy. Obstetrics and Gynecology 74: 915-920.
• [7] Sanjeevi C.B., Vivekanandan S., Narayanan P.R. 1991. Fetal response to maternal ascariasis as evidenced by anti Ascaris lumbricoides IgM antibodies in the cord blood. Acta Paediatrica Scandinavica 80: 1134-1138.
• [8] Rodrigez G.R., Rodridez G.L., Sanchez-Maldano M.I., Gomez D.G.A., Rivera C.R. 2002. Prevalence and risk factors associated with intestinal parasites in pregnant women and their relation to the infant's birth weight. Ginecologia y Obstetricia de Mexico 70: 338-348.
• [9] Devici S., Tanyuksel M., Devici G., Araz E. 2001. Spontaneous missed abortion caused by Ascaris lumbricoides. Central European Journal of Public Health 9: 188-189.
• [10] Sharma J.B., Arora B.S., Kumar S., Goel S., Dhamija A. 2001. Helminth and protozoan intestinal infections: an important cause for anemia in pregnant women in Delhi. Journal of Obstetrics and Gynecology of India 51: 58-61.
• [11] Rathi A.K., Batra S., Chandra J., Mehrota S.N., 1981. Ascaris causing intestinal obstruction in a 45 days old infant. Indian Journal of Pediatrics 18: 751-752.
• [12] da Costa-Macedo L.M., Rey L. 1990. Ascaris lumbricoides in neonate: evidence of congenital transmission of intestinal nematodes. Revista du Instituto de Medicina Tropical de Sao Paulo 32: 351-354.
• [13] Rai S.K., Rai G. 1999. Ascaris, ascariasis and its present scenario in Nepal. Journal of the Institute of Medicine 21: 191-250.
• [14] Coles G.C. 1985. Allergy and immunopathology of ascariasis. In: Ascariasis and its Public Health Significance (Eds. D.W.T. Crompton, M.C. Nesheim, Z.R. Pawlowski) London and Philadelphia, Taylor and Francis: 167-184.
• [15] Grütter M.G. 1994: Proteinase inhibitors: another new fold. Structure 2: 575-576.
• [16] Błaszkowska J. 1998. Embryotoxic and teratogenie action of trypsin inhibitor of Ascaris lumbricoides in mice. Acta Parasitologica 43: 103-108.
• [17] Błaszkowska J. 2001. Comparison of the effect of Ascaris trypsin inhibitor in various periods of mouse pregnancy. Wiadomości Parazytologiczne 47: 699-704.
• [18] Błaszkowska J. 2003. Preliminary evaluation of matemotoxic effect of Ascaris trypsin inhibitor in mice. Helminthologia 40: 195-199.
• [19] Rola F.H., Pudles J.A. 1967. Studies on the proteolytic inhibitors from Ascaris lumbricoides var. suum, purification, properties and chemical modification of the trypsin inhibitor. Archives of Biochemistry and Biophysics 120: 594-601.
• [20] Jacobsen L. 1963. A modified method for preparation of the ossified skeleton of mouse fetuses. Acta Pathologica et Microbiologica Scandinavica 53: 493-494.
• [21] Lorke D. 1977. Evaluation of skeleton. In: Methods in Prenatal Toxicology (Eds. D. Neubert, HJ. Merker, T.E. Kwasigroch), Georg Thieme Publisher, Stuttgart: 145-152.
• [22] Barrow M.V., Taylor WJ. 1969. A rapid method for detecting malformations in rat fetuses. Journal of Morphology 127: 291-30.
• [23] Wilson J.G. 1975. Reproduction and teratogenesis: current method and suggested improvements. Journal of the Association Analytical Chemistry 58: 657-667.
• [24] Khera K.S. 1984. Maternal toxicity - a possible factor in fetal malformation in mice. Teratology 31: 411-416.
• [25] Khera K.S. 1987. Maternal toxicity in humans and animals: effects on fetal development and criteria for detection. Teratogenesis, Carcinogenesis, Mutagenesis 7: 287-295.
• [26] Kadłubowski R., Ochęcka A. 1986. Proteases and their inhibitors in host-parasite system. I. The effect of Ascaris inhibitors on the selected parameters of human plasma fibrinolysis. Acta Parasitologica Polonica 30: 179-186.
• [27] Crawford G.P.M., Howse D.J., Grove D.I. 1982. Inhibition of human blood clotting by extracts of Ascaris suum. Journal of Parasitology 68: 1044-1047.