Effect of crop improvement on genetic diversity in oilseed Brassica rapa [turnip-rape] cultivars, detected by SSR markers

• Autorzy: Ofori A , Becker H C , Kopisch-Obuch F
• Języki publikacji: EN

Abstrakty

EN

With the improvement of seed quality, Brassica rapa oilseed germplasm went through 2 major breeding bottlenecks during the introgression of genes for zero erucic acid content and low glucosinolate content, respectively. This study investigates the impact of these bottlenecks on the genetic diversity in European winter B. rapa by comparing 3 open-pollinated cultivars, each representing a different breeding period. Diversity was estimated on 32 plants per cultivar, with 16 simple sequence repeat (SSR) markers covering each of the B. rapa linkage groups. There was no significant loss of genetic diversity over the 3 cultivars as indicated by allele number (ranging from 59 to 55), mean allele number (from 3.68 to 3.50), Shannon information index (from 0.94 to 0.87) and expected heterozygosity (from 0.53 to 0.48). About 83% of the total variation was attributed to within-cultivar variation, and the remaining 17% to between-cultivar variation by analysis of molecular variance (AMOVA). Individual plants were separated into the 3 cultivars by principal coordinate analysis (PCoA). In conclusion, genetic diversity within cultivars was high and quality breeding in B. rapa did not significantly reduce the genetic diversity of B. rapa winter cultivars, so there is no risk of decline in performance due to quality improvement.

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 2008
• Tom: 49
• Numer: 3
• Opis fizyczny: p.207-212,fig.,ref.

Twórcy

autor

Ofori A

• Department of Crop Sciences, Georg-August-University Gottingen, Von-Siebold-Str.8, 37075, Gottingen, Germany

autor

Becker H C

• Department of Crop Sciences, Georg-August-University Gottingen, Von-Siebold-Str.8, 37075, Gottingen, Germany

autor

Kopisch-Obuch F

• Plant Breeding Institute, Christian-Albrechts-University, Kiel, Germany

Bibliografia

• Das S, Rajagopal J, Bhatia S, Srivastava PS, Lakshmikumaran M, 1999. Assessment of genetic variation within Brassica campestris cultivars using amplified fragment length polymorphism and random amplification of polymorphic DNA markers. J Biosciences 24: 433-440.
• Dewan DB, Rakow G, Downey RK, 1998. Growth and yield of doubled haploid lines of oilseed Brassica rapa. Can. J. Plant Sci. 78: 537-544.
• Dice LF, 1945. Measures of the amount of ecological association between species. Ecology 26: 297-302.
• Downey RK, 1964. A selection of Brassica campestris L. containing no erucic acid in its seed oil. Can J Plant Sci 44: 295.
• Falconer DS, Mackay TFC, 1996. Introduction to quantitative genetics, 4th edition. Harlow (Essex, UK): Addison Wesley Longman.
• Fu YB, Peterson GW, Richards KW, Somers D, DePauw RM, Clarke JM, 2005. Allelic reduction and genetic shift in the Canadian hard red spring wheat germplasm released from 1845 to 2004. Theor Appl Genet 110: 1505-1516.
• Fu YB, Peterson GW, Scoles G, Rossnagel B, Schoen DJ, Richards KW, 2003. Allelic diversity changes in 96 Canadian oats cultivars released from 1886 to 2001. Crop Sci 43: 1989-1995.
• Gu HH, Zhou WJ, Hagberg P, 2003. High frequency spontaneous production of doubled haploid plants in microspore cultures of Brassica rapa spp. chinensis. Euphytica 134: 239-245.
• Harlan JR, 1972. Genetics of disaster. J Environ Qual 1: 212-215.
• Koebner RM, Donini DP, Reeves JC, Cooke RJ, Law JR, 2003. Temporal flux in the morphological and molecular diversity of UK barley. Theor Appl Genet 106: 550-558.
• Khlestkina EK, Huang XQ, Quenum FJB, Chebotar S, Röder MS, Börner A, 2004. Genetic diversity in cultivated plant-loss or stability? Theor Appl Genet 108: 1466-1472.
• Krzymanski J, 1970. Inheritance of thioglucoside content by rapeseed (Brassica napus). Journees Internationales sur le Colza. C.E.T.I.O.M. 212-218.
• Lu H, Bernardo R, 2001. Molecular marker diversity among current and historical maize inbreds. Theor Appl Genet 103: 613-617.
• Ofori A, Becker HC, 2007. Use of Brassica rapa resources for biomass production: Heterosis in populations crosses. In: Proc Plant Genetic Resources and their Exploitation in the Plant Breeding of Food and Agriculture. 18th EUCARPIA Genetic Resources Section Meeting, 23-26 May 2007, Piesťany, Slovak Republic.
• Ofori A, Becker HC, 2008. Breeding of Brassica rapa for biogas production: Heterosis and combining ability of biomass yield. Bioenerg Res DOI 10.1007/ s 12155-008-9001-2.
• Peakall R, Smouse PE, 2006. GENALEX 6: genetic analysis in excel, population software for teaching and research. Mol Ecol Notes 6: 288-295.
• Persson K, Fait AS, Bothmer RV, 2001. Genetic diversity of allozymes in turnip (Brassica rapa L. var. rapa) from the Nordic area. Hereditas 134: 43-52.
• Piquemal J, Cinquin E, Couton F, Rondeau C, Seignoret E, 2005. Construction of an oilseed rape (Brassica napus L.) genetic map with SSR markers. Theor Appl Genet 111: 1514-1523.
• Plieske J, Struss D, 2001. Microsatellite markers for genome analysis in Brassica. I. Development in Brassica napas and abundance in Brassicaceae species. Theor Appl Genet 102: 689-694.
• Reiner H, Holzner W, Ebermann R, 1995. The development of turnip-type and oilseed-type Brassica rapa crops from wild-type in European overview of botanical, historical and linguistic facts. Rapeseed Today Tomorrow, 9th International Rapeseed Congress, Cambridge, UK, 4: 1066-1069.
• Rohlf FJ, 2001. NTSYS-pc numerical taxonomy and multivariate analysis system. Exeter Software, New York, USA: Exeter Software.
• Roussel V, Koenig J, Bechert M, Baifourier F, 2004. Molecular diversity in French bread wheat accessions related to temporal trends and breeding programmes. Theor App Genet 108: 920-930.
• Rudolph B, 2001. Entwicklung, Charakterisierung und genetische Kartierung von Mikrosatelliten-Markern beim Raps (Brassica napus L.). PhD thesis, Faculty of Mathematics and Sciences, Georg-August University of Göttingen.
• Snowdon RJ, Friedt W, 2004. Molecular markers in Brassica oilseed breeding: current status and future possibilities. Plant Breeding 123: 1-8.
• Tonguç M, Griffiths PD, 2004. Genetic relationships of Brassica vegetables determined using database derived sequence repeats. Euphytica 137: 193-201.
• Uzunova MI, Ecke W, 1999. Abundance, polymorphism, and genetic mapping of microsatellites in oilseed rape (Brassica napus L.). Plant Breeding 118: 323-326.
• Yap I, Nelson RJ, 1996. Winboot: a program for performing bootstrap analysis of binary data to determine the confidence limits of UPGMA-based dendrograms. IRRI. Discussion Paper Series no 14, International Rice Institute, Manila, Philippines.
• Zhang GQ, Tang GX, Song WJ, Zhou WJ, 2004. Resynthesizing Brassica napus from interspecific hybridization between Brassica rapa and B. oleracea through ovary culture. Euphytica 140: 181-187.
• Zhao J, Becker HC, 1998. Genetic variation in Chinase and European oilseed rape (B. napus) and turnip rape (B. campestris) analysed with isozymes. Acta Agron Sin 24:213-220.
• Zhao J, Wang X, Deng B, Lou P, Wu J, Sun R, et al. 2005. Genetic relationships within Brassica rapa as inferred from AFLP fingerprints. Theor Appl Genet 110: 1301-1314.
• Zhou WJ, Zhang GQ, Tuvesson S, Dayteg C, Gertsson B, 2006. Genetic survey of Chinese and Swedish oilseed rape (Brassica napus L.) by simple sequence repeats (SSRs). Genet Resour Crop Ev 53: 443-447.