Efektywność pozyskiwania zarodków w zależności od miejsca deponowania nasienia w rui indukowanej u krów dawczyń

• Autorzy: Zychlinska-Buczek J. , Jaskowski J.M. , Gil Z. , Herod Z.

Warianty tytułu

EN

Influence of semen placement location and other factors on the number and morphological status of obtained embryos from donor cows

• Języki publikacji: PL

Abstrakty

EN

The aim of the study was to analyze the effectiveness of embryo collection depending on where semen in superovulation cow-donors in heat is deposited. Research was carried out on 44 embryo donors, including 26 heifers and 18 cows of the Polish HolsteinFriesian breed. Manipulations were carried out with the participation of veterinary doctors who specialize in embryo transfer. The estrus cycle of the future donors was synchronized with the double intramuscular injection of 2 ml Cloprostenol (Oestrophan, Biofaktor Company), Dinolytic (Pfizer Polska Sp. z o.o.) 5 ml or Bioestrovet (0.5 mg of Cloprostenol – active substance) (Vetoquinol, Biowet Sp. z o.o.) 2 ml administered for 11 days. Superovulation was initiated at day 10th of an estrus cycle – administered by injection of 10 ml of FSH (Pluset) during 4 consecutive days (in the morning and in the evening). Estrus was induced on the 3rd day of the superovulation treatment by providing 500 µg and 250 µg of Cloprostenol (Bioestrovet) twice – 2 ml in the morning and 1 ml in the evening. Subsequently, 25 donors were inseminated three times, another 19 were inseminated twice with single or double doses of semen deposited at different locations inside the uterus. Donors were inseminated with frozen semen which is used for standard AI. Obtained results showed a significant influence of placement location of semen on the efficiency of embryos’ collection. The highest ratio (40.4%) of embryos and oocytes was obtained in the case when semen was itself placed in the uterine horn. The lowest ratio (16.2%) was observed in the case when semen was placed in the uterus. Moreover, a difference in the number of obtained oocytes and embryos depends on the sequence of semen placement locations. In the case of location in the uterus body versus uterine horn 0.6 more embryos and oocytes were collected. The number of embryos suitable for transfer was the highest when semen was placed in the uterine horn. Concluding, the uterine horn appeared to be the best place for semen location.

Słowa kluczowe

PL

bydlo; rozrod zwierzat; przenoszenie zarodkow; krowy dawczynie; ruja indukowana; superowulacja; inseminacja; miejsce zdeponowania nasienia; zarodki bydlece; pozyskiwanie zarodkow; efektywnosc

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2016
• Tom: 72
• Numer: 09
• Opis fizyczny: s.587-589,tab.,bibliogr.

Twórcy

autor

Zychlinska-Buczek J.

• Instytut Nauk o Zwierzętach, Zakład Hodowli Bydła, Wydział Hodowli i Biologii Zwierząt, Uniwersytet Rolniczy w Krakowie, al.Mickiewicza 24/28, 30-059 Kraków

autor

Jaskowski J.M.

• Instytut Weterynarii, Wydział Medycyny Weterynaryjnej i Nauk o Zwierzętach, Uniwersytet Przyrodniczy w Poznaniu, ul.Wołyńska 35, 60-637 Poznań

autor

Gil Z.

• Instytut Nauk o Zwierzętach, Zakład Hodowli Bydła, Wydział Hodowli i Biologii Zwierząt, Uniwersytet Rolniczy w Krakowie, al.Mickiewicza 24/28, 30-059 Kraków

autor

Herod Z.

• Instytut Nauk o Zwierzętach, Zakład Hodowli Bydła, Wydział Hodowli i Biologii Zwierząt, Uniwersytet Rolniczy w Krakowie, al.Mickiewicza 24/28, 30-059 Kraków

Bibliografia

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• Carvalho P. D., Souza A. H., Sartori R., Hackbart K. S., Dresch A. R., Vieira L. M., Baruselli P. S., Guenther J. N., Fricke P. M., Shaver R. D., Wiltbank M. C.: Effects of deep-horn AI on fertilization and embryo production in superovulated cows and heifers. Theriogenology 2013, 80, 1074-1081.
• Hawk H. W., Tanabe T. Y.: Effect of unilateral cornual insemination upon fertilization rate in superovulating and single ovulating cattle. J. Anim. Sci. 1986, 63, 551-560.
• Kaimio I., Mikkola M., Lindeberg H., Heikkinen J., Hasler J. F., Taponen J.: Embryo production with sex-sorted semen in superovulated dairy heifers and cows. Theriogenology 2013, 80, 950-954.
• Kurykin J., Jaakma Ü., Jalakas M., Aidnik M., Waldmann A., Majas L.: Pregnancy percentage following deposition of sex-sorted sperm at different sites within the uterus in estrus-synchronized heifers. Theriogenology 2007, 67, 754-759.
• Lopez-Gatius F.: Site of semen deposition in cattle: a review. Theriogenology 2000, 53, 1407-1414.
• McKenna T., Lenz R. W., Fenton S. E., Ax R. L.: Nonreturn rates of dairy cattle following uterine body or cornual insemination. J. Dairy Sci. 1990, 73, 1779-1783.
• Schenk J. L., Suh T. K., Seidel G. E. Jr.: Embryo production from superovulated cattle following insemination of sexed sperm. Theriogenology 2006, 65, 299-307.
• Senger P. L., Becker W. C., Davidge S. T., Hillers J. K., Reeves J. J.: Influence of cornual insemination on conception in dairy cattle. J. Anim. Sci. 1988, 66, 3010-3016.
• Soom A. van, Verberckmoes S.: Deep intrauterine insemination in cattle. Gynecol. Obstet. Fertil. 2004, 32, 911-915.
• Williams B. L., Gwazdauskas F. C., Whittier W. D., Pearson R. E., Nebel R. L.: Impact of site of inseminate deposition and environmental factors that influence reproduction of dairy cattle. J. Dairy Sci. 1988, 71, 2278-2283.
• Zobel R., Gereš D., Pipal I., Buić V., Gračner D., Tkalcic S.: Influence of the semen deposition site on the calves’ sex ratio in Simmental dairy cattle. Reprod. Domest. Anim. 2011, 46, 595-601.

Identyfikatory

DOI

10.21521/mw.5556