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2011 | 61 | 4 |
Tytuł artykułu

SE-HPLC-DAD analysis of flaxseed lignan macromolecule and its hydrolysates

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
A lignan macromolecule (LM) was extracted from defatted flaxseeds using an ethanol-dioxan system (1:1, v/v) and purified using Amberlite column chromatography with water and methanol as mobile phases. The LM was subjected to chemical hydrolysis (base, acid, base & acid), as well as to enzymatic processing using pepsin, pancreatin, cellulase, and β-glucuronidase. The study revealed that lignan macromolecule in flaxseed was not homogenous. The chemical hydrolysis as well as enzymatic treatment using β-glucuronidase and cellulase released low molecular phenolic compounds from the lignan macromolecule. The liberation of secoisolariciresinol (SECO) and free phenolic acids (p-coumaric and ferulic acids) from flaxseed lignan macromolecule as a result of the base and acid hydrolyses was noted. The application of pepsin and pancreatin did not change the composition of the lignan macromolecule.
Słowa kluczowe
EN
Wydawca
-
Rocznik
Tom
61
Numer
4
Opis fizyczny
p.263-271,fig.,ref.
Twórcy
autor
  • Division of Food Sciences, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Tuwima 10, 10-747 Olsztyn, Poland
autor
autor
autor
Bibliografia
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