SE-HPLC-DAD analysis of flaxseed lignan macromolecule and its hydrolysates

• Autorzy: Kosinska A. , Urbalewicz A. , Penkacik K. , Karamac M. , Amarowicz R.
• Języki publikacji: EN

Abstrakty

EN

A lignan macromolecule (LM) was extracted from defatted flaxseeds using an ethanol-dioxan system (1:1, v/v) and purified using Amberlite column chromatography with water and methanol as mobile phases. The LM was subjected to chemical hydrolysis (base, acid, base & acid), as well as to enzymatic processing using pepsin, pancreatin, cellulase, and β-glucuronidase. The study revealed that lignan macromolecule in flaxseed was not homogenous. The chemical hydrolysis as well as enzymatic treatment using β-glucuronidase and cellulase released low molecular phenolic compounds from the lignan macromolecule. The liberation of secoisolariciresinol (SECO) and free phenolic acids (p-coumaric and ferulic acids) from flaxseed lignan macromolecule as a result of the base and acid hydrolyses was noted. The application of pepsin and pancreatin did not change the composition of the lignan macromolecule.

• Wydawca: -
• Czasopismo: Polish Journal of Food and Nutrition Sciences
• Rocznik: 2011
• Tom: 61
• Numer: 4
• Opis fizyczny: p.263-271,fig.,ref.

Twórcy

autor

Kosinska A.

• Division of Food Sciences, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Tuwima 10, 10-747 Olsztyn, Poland

autor

Urbalewicz A.

autor

Penkacik K.

autor

Karamac M.

autor

Amarowicz R.

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