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2010 | 70 | 1 |
Tytuł artykułu

Spatial and temporal changes of osteopontin in oxygen-glucose-deprived hippocampal slice cultures

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Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
We have examined the temporal changes and cellular localization of osteopontin (OPN) mRNA and protein in organotypic hippocampal slice cultures subjected to ischemia-like oxygen–glucose deprivation (OGD). The sequential induction pattern of OPN expression occurred in a time- and cell-dependent manner in hippocampal slice cultures after OGD. The early response consisted of neuronal and microglial OPN upregulation, followed by a later extended phase of expression in reactive astrocytes. OPN immunoreactivity after OGD matched the mRNA induction patterns. Activated microglia revealed OPN staining in focal deposits, whereas neurons and reactive astrocytes showed perinuclear staining with a punctate cytosolic pattern of OPN, typical of secreted proteins. These data demonstrated that the temporal and cellular patterns of OPN induction in reactive glial cells in this in vitro model closely correlated with that in the in vivo model, suggesting that OPN has a multifunctional role in the pathogenesis of ischemic injury.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
70
Numer
1
Opis fizyczny
p.1-12,fig.,ref.
Twórcy
autor
  • Department of Anatomy, College of Medicine, The Catholic University of Korea, Seoul, South Korea
autor
  • Department of Anatomy, College of Medicine, The Catholic University of Korea, Seoul, South Korea
autor
  • Department of Anatomy, College of Medicine, The Catholic University of Korea, Seoul, South Korea
autor
  • Department of Anatomy, College of Medicine, The Catholic University of Korea, Seoul, South Korea
autor
  • Department of Anatomy, College of Medicine, The Catholic University of Korea, Seoul, South Korea
autor
  • Department of Anatomy, College of Medicine, The Catholic University of Korea, Seoul, South Korea
autor
  • Department of Anatomy, College of Medicine, The Catholic University of Korea, Seoul, South Korea
autor
  • Department of Anatomy, College of Medicine, The Catholic University of Korea, Seoul, South Korea
Bibliografia
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