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2010 | 70 | 1 |

Tytuł artykułu

Spatial and temporal changes of osteopontin in oxygen-glucose-deprived hippocampal slice cultures

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
We have examined the temporal changes and cellular localization of osteopontin (OPN) mRNA and protein in organotypic hippocampal slice cultures subjected to ischemia-like oxygen–glucose deprivation (OGD). The sequential induction pattern of OPN expression occurred in a time- and cell-dependent manner in hippocampal slice cultures after OGD. The early response consisted of neuronal and microglial OPN upregulation, followed by a later extended phase of expression in reactive astrocytes. OPN immunoreactivity after OGD matched the mRNA induction patterns. Activated microglia revealed OPN staining in focal deposits, whereas neurons and reactive astrocytes showed perinuclear staining with a punctate cytosolic pattern of OPN, typical of secreted proteins. These data demonstrated that the temporal and cellular patterns of OPN induction in reactive glial cells in this in vitro model closely correlated with that in the in vivo model, suggesting that OPN has a multifunctional role in the pathogenesis of ischemic injury.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

70

Numer

1

Opis fizyczny

p.1-12,fig.,ref.

Twórcy

autor
  • Department of Anatomy, College of Medicine, The Catholic University of Korea, Seoul, South Korea
autor
  • Department of Anatomy, College of Medicine, The Catholic University of Korea, Seoul, South Korea
autor
  • Department of Anatomy, College of Medicine, The Catholic University of Korea, Seoul, South Korea
autor
  • Department of Anatomy, College of Medicine, The Catholic University of Korea, Seoul, South Korea
autor
  • Department of Anatomy, College of Medicine, The Catholic University of Korea, Seoul, South Korea
autor
  • Department of Anatomy, College of Medicine, The Catholic University of Korea, Seoul, South Korea
autor
  • Department of Anatomy, College of Medicine, The Catholic University of Korea, Seoul, South Korea
autor
  • Department of Anatomy, College of Medicine, The Catholic University of Korea, Seoul, South Korea

Bibliografia

  • Borges K, Gearing M, Rittling S, Sorensen ES, Kotloski R, Denhardt ET, Dingledine R (2008) Characterization of osteopontin expression and function after status epilepti- cus. Epilepsia 49: 1675-1685.
  • Chabas D, Baranzini SE, Mitchell D, Bernard CC, Rittling SR, Denhardt DT, Sobel RA, Lock C, Karpuj M, Pedotti R, Heller R, Oksenberg JR, Steinman L (2001) The influ­ence of the proinflammatory cytokine, osteopontin, on autoimmune demyelinating disease. Science 294: 1731­1735.
  • Chechneva O, Dinkel K, Cavaliere F, Martinez-Sanchez M, Reymann KG (2006) Anti-inflammatory treatment in oxygen-glucose-deprived hippocampal slice cultures is neuroprotective and associated with reduced cell prolif­eration and intact neurogenesis. Neurobiol Dis 23: 247­259.
  • Choi JS, Kim HY, Cha JH, Choi JY, Lee MY (2007) Transient microglial and prolonged astroglial upregula- tion of osteopontin following transient forebrain ischemia in rats. Brain Res 1151: 195-202.
  • Chellaiah MA, Hruska KA (2002) The integrin avP3 and CD44 regulate the actions of osteopontin on osteoclast motility. Calcif Tissue Int 72: 197-205.
  • Doyle KP, Yang T, Lessov NS, Ciesielski TM, Stevens SL, Simon RP, King JS, Stenzel-Poore MP (2008) Nasal administration of osteopontin peptide mimetics confers neuroprotection in stroke. J Cereb Blood Flow Metab 28: 1235-1248.
  • Ellison JA, Velier JJ, Spera P, Jonak ZI, Wang X, Barone FC, Feuerstein GZ (1998) Osteopontin and its integrin recep­tor alphabeta3 are upregulated during formation of the glial scar after focal stroke. Stroke 29: 1698-1707.
  • Ellison JA, Barone FC, Feuerstein GZ (1999) Matrix remod­eling after stroke. De novo expression of matrix proteins and integrin receptors. Ann N Y Acad Sci 890: 204-222.
  • Holopainen IE (2005) Organotypic hippocampal slice cul­tures: a model system to study basic cellular and molecu­lar mechanisms of neuronal cell death, neuroprotection, and synaptic plasticity. Neurochem Res 30: 1521-1528.
  • Kang WS, Choi JS, Shin YJ, Kim HY, Cha JH, Lee JY, Chun MH, Lee MY (2008) Differential regulation of osteopon­tin receptors, CD44 and the alpha(v) and beta(3) integrin subunits, in the rat hippocampus following transient fore- brain ischemia. Brain Res 1228: 208-216.
  • Laake JH, Haug FM, Wieloch T, Ottersen OP (1999) A simple in vitro model of ischemia based on hippocampal slice cultures and propidium iodide fluorescence. Brain Res Protoc 4: 173-184.
  • Lee MY, Shin SL, Choi YS, Kim EJ, Cha JH, Chun MH, Lee SB, Kim SY (1999) Transient upregulation of osteopontin mRNA in hippocampus and striatum following global forebrain ischemia in rats. Neurosci Lett 271: 81-84.
  • Maetzler W, Berg D, Schalamberidze N, Melms A, Schott K, Mueller JC, Liaw L, Gasser T, Nitsch C (2007) Osteopontin is elevated in Parkinson's disease and its absence leads to reduced neurodegeneration in the MPTP model. Neurobiol Dis 25: 473-482.
  • Meller R, Stevens SL, Minami M, Cameron JA, King S, Rosenzweig H, Doyle K, Lessov NS, Simon RP, Stenzel- Poore MP (2005) Neuroprotection by osteopontin in stroke. J Cereb Blood Flow Metab 25: 217-225.
  • Noraberg J, Poulsen FR, Blaabjerg M, Kristensen BW, Bonde C, Montero M, Meyer M, Gramsbergen JB, Zimmer J (2005) Organotypic hippocampal slice cultures for studies of brain damage, neuroprotection and neurore- pair. Curr Drug Targets CNS Neurol Disord 4: 435-452.
  • Schroeter M, Zickler P, Denhardt DT, Hartung HP, Jander S (2006) Increased thalamic neurodegeneration following ischaemic cortical stroke in osteopontin-deficient mice. Brain 129: 1426-1437.
  • Shin T, Ahn M, Kim H, Moon C, Kang TY, Lee JM, Sim KB, Hyun JW (2005) Temporal expression of osteopontin and CD44 in rat brains with experimental cryolesions. Brain Res 11: 95-101.
  • Stoppini L, Buchs PA, Muller D (1991) A simple method for organotypic cultures of nervous tissue. J Neurosci Methods 37: 173-182.
  • Suzuki K, Zhu B, Rittling SR, Denhardt DT, Goldberg HA, McCulloch CA, Sodek J (2002) Colocalization of intrac- ellular osteopontin with CD44 is associated with migra­tion, cell fusion, and resorption in osteoclasts. J Bone Miner Res 17: 1486-1497.
  • Wang X, Louden C, Yue TL, Ellison JA, Barone FC, Solleveld HA, Feuerstein GZ (1998) Delayed expression of osteopontin after focal stroke in the rat. J Neurosci 18: 2075-2083.
  • Zhu B, Suzuki K, Goldberg HA, Rittling SR, Denhardt DT, McCulloch CA, Sodek J (2004) Osteopontin modulates CD44-dependent chemotaxis of peritoneal macrophages through G-protein-coupled receptors: evidence of a role for an intracellular form of osteopontin. J Cell Physiol 198: 155-167.
  • Zohar R, Suzuki N, Suzuki K, Arora P, Glogauer M, McCulloch CA, Sodek J (2000) Intracellular osteopontin is an integral component of the CD44-ERM complex involved in cell migration. J Cell Physiol 184: 118-130.

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Bibliografia

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