Heterologous expression of hemagglutinin-neuraminidase protein from Newcastle disease virus strain AF2240 in Centella asiatica

• Autorzy: Lai K. S. , Yusoff K. , Mahmood M.
• Języki publikacji: EN

Abstrakty

EN

We explored the use of the medicinally important plant Centella asiatica for expression of hemagglutinin-neuraminidase (HN) protein of Newcastle disease virus (NDV) strain AF2240. HN protein is the principal target for subunit vaccine development against NDV. The full-length HN gene was cloned into a plant expression construct driven by the CaMV 35S promoter and C-terminal fusion of green fluorescence protein (GFP) as reporter system. The recombinant expression construct was transformed via particle bombardment into C. asiatica callus. Transformants were screened using GFP and selected on MS medium supplemented with 15 mg/l hygromycin. The ~1.8 kb HN mRNA transcript was detected on the putative transformants using RT-PCR. The presence of HN protein expression was further confirmed through dot blot analysis using anti-NDV chicken serum. Here we report, for the first time, the use of a novel medicinal plant as a new platform for HN protein expression.

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 2012
• Tom: 54
• Numer: 1
• Opis fizyczny: p.142-147,fig.,ref.

Twórcy

autor

Lai K. S.

• Department of Biochemistry, University Putra Malaysia (UPM), 43400 Serdang, Selangor DE, Malaysia

autor

Yusoff K.

autor

Mahmood M.

Bibliografia

• FISHER R, EMANS NJ, TWYMAN RM, and SCHILLBERG S. 2004. Molecular farming in plants: technology platforms. In:Goodman RB [ed.], Encyclopedia of Plant and CropScience, 753–756. New York, Marcel Dekker.
• GABRIEL P, and ZISHENG Z. 2010. Strategies for high-level recombinant protein expression in transgenic microalgae:A review. Biotechnology Advances 28: 910–918.
• GABRIEL DP, ROBERT CB, and WEI WS. 2006. Purification of GFP fusion proteins from transgenic plant cell cultures.Protein Expression and Purification 49: 183–189.
• HONG YL, and MEE LC. 2009. Use of GFP to investigate expression of plant-derived vaccines. In: Hicks BW [ed.],Methods in Molecular Biology, Viral Applications of
• Green Fluorescent Protein, 275–285, vol 515. Human Press, Totowa.
• IDERIS A, IBRAHIM AL, and SPRADBROW PB. 1990. Vaccination of chicken against Newcastle Disease Virus with a food pelletvaccine. Avian Pathology 19: 371–384.
• KIM OT, BANG KH, SHIN YS, LEE MJ, JUNG SJ, HYUN DY, KIM YC, SEONG NS, CHA SW, and HWANG B. 2007. Enhanced productionof asiaticoside from hairy root cultures ofCentella asiatica (L.) Urban elicited by methyl jasmonate.Plant Cell Reports 26: 1941–1949.
• KIM OT, KIM HS, OHYAMA K, MURANAKA T, CHOI YE, LEE HY, KIM MY, and HWANG B. 2010. Upregulation of phytosterol and triterpene biosynthesis in Centella asiatica hairy roots overexpressed ginseng farnesyl diphosphate synthase. Plant Cell Reports 29: 403–411.
• KO K, and KOPROWSKI H. 2005. Plant biopharming of monoclonal antibodies. Virus Research 111: 93–100.
• KRISHNAN VN, SONI KB, and RAJMOHAN K. 2008. Agrobacterium tumefaciens mediated genetic transformation in Centellaasiatica L. Urban. Current Biotica 2: 1–8.
• LAI KS, ABDULLAH P, YUSOFF K, and MAHMOOD M. 2011a. An efficient protocol for particle bombardment–mediatedtransformation of Centella asiatica callus. ActaPhysiologiae Plantarum 33: 2547–2552.
• LAI KS, YUSOFF K, and MAHMOOD M. 2011b. Extracellular matrix as the early structural marker for Centella asiaticaembryogenic tissues. Biologia Plantarum 55:549–553.
• LHERNOULD S, LABROUSSE P, LEJOLLY D, LÉONARD R, CARLUÉ M, and COSTA G. 2008. In vivo activity of recombinanthuman Lewis fucosyltransfease III in leaves of Nicotianatabacum L. Biologia Plantarum 52: 267–274.
• MARTIN KP. 2004. Plant regeneration through somatic embryogenesis in medicinally important Centella asiaticaL. In Vitro Cellular Developmental Biology-Plant 40: 586–591.
• MILEN IG, JOST W, and ALEXANDRE M. 2009. Bioprocessing of plant cell cultures for mass production of targeted compounds.Applied Microbiology and Biotechnology 83.809–823
• MOHAMMAD RS, RAINER F, and ULRICH C. 2009. Recombinantantibody- mediated resistance against Tomato yellow leafcurl virus in Nicotiana benthamiana. Archives ofVirology 154: 457–467.
• MURASHIGE T, and SKOOG F. 1962. A revised medium for rapid growth of and bioassay with tobacco tissue cultures.Physiologia Plantarum 15: 473–497.
• MURPHY FA, FAUQUET CM, BISHOP DHL, GHABRIAL, SA, JARVIS AW, MARTELLI GP, MAYO MA, and SUMMERS MD. 1995.Virus Taxonomy. Classification and Nomenclature ofViruses. Sixth Report of the International Committee onTaxonomy of Viruses. Archives of Virology [Supplement]10, 268–274. Springer, Vienna, New York.146
• Lai et al.ONG HKA, ALI AM, OMAR AR, and YUSOFF K. 2000. Cloning and expression of the HN gene from the velogenic viscerotropicNewcastle disease virus strain AF 2240 in Sf9insect cells. Cytotechnology 32: 243–251.
• REYNOLDS DL, and MARAQA AD. 2000. Protective immunity against Newcastle disease: The role of antibodies specificto Newcastle disease virus polypeptides. AvianDiseases 44 : 138–144.
• SORIA-GUERRA RE, MORENO-FIERROS L, and ROSLAES-MENDOZA S. 2011. Two decades of plant-based candidates vaccine: areview of the chimeric protein approaches. Plant CellReports 30:1367–1382.
• TAN WS, LAU CH, NG KB, IBRAHIM AL, and YUSOFF K. 1995. Nucleotide sequence of the haemagglutinin-neuraminidase(HN) gene of a Malaysian heat resistant viscerotropic-velogenic Newcastle disease virus. DNASequence 6: 47–50.
• WONG SK, TAN WS, OMAR AR, TAN CS, and YUSOFF K. 2009. Immunogenic properties of recombinant ectodomain of Newcastle disease virus hemagglutinin-neuraminidase protein expressed in Escherichia coli. Acta Virologica53: 35–41.
• YUSOFF K, NESBIT M, MCCARTNEY H, EMMERSON PT, and SAMSON ACR. 1988. Mapping of three antigenic sites onthe hemagglutinin-neuraminidase protein of Newcastledisease virus. Virus Research 11: 319–333

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