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2012 | 54 | 1 |

Tytuł artykułu

Heterologous expression of hemagglutinin-neuraminidase protein from Newcastle disease virus strain AF2240 in Centella asiatica

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Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
We explored the use of the medicinally important plant Centella asiatica for expression of hemagglutinin-neuraminidase (HN) protein of Newcastle disease virus (NDV) strain AF2240. HN protein is the principal target for subunit vaccine development against NDV. The full-length HN gene was cloned into a plant expression construct driven by the CaMV 35S promoter and C-terminal fusion of green fluorescence protein (GFP) as reporter system. The recombinant expression construct was transformed via particle bombardment into C. asiatica callus. Transformants were screened using GFP and selected on MS medium supplemented with 15 mg/l hygromycin. The ~1.8 kb HN mRNA transcript was detected on the putative transformants using RT-PCR. The presence of HN protein expression was further confirmed through dot blot analysis using anti-NDV chicken serum. Here we report, for the first time, the use of a novel medicinal plant as a new platform for HN protein expression.

Wydawca

-

Rocznik

Tom

54

Numer

1

Opis fizyczny

p.142-147,fig.,ref.

Twórcy

autor
  • Department of Biochemistry, University Putra Malaysia (UPM), 43400 Serdang, Selangor DE, Malaysia
autor
autor

Bibliografia

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  • Green Fluorescent Protein, 275–285, vol 515. Human Press, Totowa.
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  • KIM OT, KIM HS, OHYAMA K, MURANAKA T, CHOI YE, LEE HY, KIM MY, and HWANG B. 2010. Upregulation of phytosterol and triterpene biosynthesis in Centella asiatica hairy roots overexpressed ginseng farnesyl diphosphate synthase. Plant Cell Reports 29: 403–411.
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  • KRISHNAN VN, SONI KB, and RAJMOHAN K. 2008. Agrobacterium tumefaciens mediated genetic transformation in Centellaasiatica L. Urban. Current Biotica 2: 1–8.
  • LAI KS, ABDULLAH P, YUSOFF K, and MAHMOOD M. 2011a. An efficient protocol for particle bombardment–mediatedtransformation of Centella asiatica callus. ActaPhysiologiae Plantarum 33: 2547–2552.
  • LAI KS, YUSOFF K, and MAHMOOD M. 2011b. Extracellular matrix as the early structural marker for Centella asiaticaembryogenic tissues. Biologia Plantarum 55:549–553.
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