EN
93 environmental samples were collected and tested using molecular biology techniques. The presence of chromosomal sequence Ba813 was detected in three soil samples (SS1, SS3, ZL2) and in three dental pulp samples (ZZ3, ZZ5, ZZ8). The presence of pag gene (210 bp) was observed in SS3 and ZL2 samples. There were no positive reactions with primers targeting the capsule encoding gene (777 bp). Multiplex PCR reaction enables identification of capC gene (264 bp) in five samples: SS3, ZL2, ZZ3, ZZ5, ZZ8. The presence of pag gene (SS3, ZL2) and cap gene (SS3, ZL2, ZZ5) were observed in standard PCR. Bacillus anthracis spores were detected by SMART test in three samples: SS3, ZL2 and ZZ5. These strains were non-hemolytic, sensitive to penicillin and gamma phage. The PCR positive for pag gene isolates were able to produce PA (protective antigen).