Wystepowanie przetrwalnikow Bacillus anthracis w srodowisku

• Autorzy: Bielawska-Drozd A , Bartoszcze M.

Warianty tytułu

EN

Bacillus anthracis spores in the environment

• Języki publikacji: PL

Abstrakty

EN

93 environmental samples were collected and tested using molecular biology techniques. The presence of chromosomal sequence Ba813 was detected in three soil samples (SS1, SS3, ZL2) and in three dental pulp samples (ZZ3, ZZ5, ZZ8). The presence of pag gene (210 bp) was observed in SS3 and ZL2 samples. There were no positive reactions with primers targeting the capsule encoding gene (777 bp). Multiplex PCR reaction enables identification of capC gene (264 bp) in five samples: SS3, ZL2, ZZ3, ZZ5, ZZ8. The presence of pag gene (SS3, ZL2) and cap gene (SS3, ZL2, ZZ5) were observed in standard PCR. Bacillus anthracis spores were detected by SMART test in three samples: SS3, ZL2 and ZZ5. These strains were non-hemolytic, sensitive to penicillin and gamma phage. The PCR positive for pag gene isolates were able to produce PA (protective antigen).

Słowa kluczowe

PL

wystepowanie; Bacillus anthracis; laseczki waglika; bakterie; przetrwalniki bakteryjne; wykrywanie

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2007
• Tom: 63
• Numer: 08
• Opis fizyczny: s.946-950,fot.,bibliogr.

Twórcy

autor

Bielawska-Drozd A

• Wojskowy Instytut Higieny i Epidemiologii, ul.Lubelska 2, 24-100 Pulawy

autor

Bartoszcze M.

Bibliografia

• 1. Benda B., Korsgern O.: Interleukin-6 in islet xenograft rejection. Transpl. Int. 2001, 14, 63-71.
• 2. Blancho G., Gianello P., Germana S., Baetschert M., Sachs D. H., Leguern C.: Molecular identification of porcine interleukin 10: Regulation of expression in a kidney allograft model. Proc. Natl Acad. Sci. 1995, 92, 2800-2804.
• 3. Bühler L., Friedman T., Iacomini J., Cooper D. K. C.: Xenotransplantation - state of the hear - update 1999. Frontiers Biosci. 1999, 4, 416-432.
• 4. Cooper D. K., Koren E., Oriol R.: Oligosaccharides and discordant xenotransplantation. Immunol Rev. 1994, 141, 31-58.
• 5. Darwich L., Balasch M., Plana-Durán J., Segalés J., Domingo M., Mateu E.: Cytokine profiles of peripheral blood mononuclear cells from pigs with postweaning multisystemic wasting syndrome in response to mitogen, superantigen or recall viral antigens. J. Gen. Virol. 2003, 84, 3453-3457.
• 6. De Groot J., Kruijt L., Scholten J. W., Boersma W. J. A., Buist W. G., Engel B., van Reenen C. G.: Age, gender and litter-related variation in T-lymphocyte cytokine production in young pigs. Immunology 2005, 115, 495 505.
• 7. Deol H. S., Tuch B. E.: Effect of interleukin-10 on human anti-porcine xenogeneic cellular response in vitro. Transplantation 2000, 69, 112-119.
• 8. Deschamps J. Y., Roux J. Y., Sai P., Gouin E.: History of xenotransplantation. Xenotransplantation 2005, 12, 91-109.
• 9. Galili U., Mandrell R. E., Hamadeh R. M., Shohet R. B., Griffiss J. M.: The interaction between the human natural anti-a galactosyl IgG (anti-Gal) and bacteria of the human flora. Infect. Immun. 1988, 57, 1730-1737.
• 10. Hammerman M. R.: Xenotransplantation of developing kidneys. Am. J. Physiol. Renal. Physiol. 2002, 283, 601-606.
• 11. Karlson-Parra A., Ridderstad A., Wallgren A. C., Moller E., Ljunggren H. G., Korsgren O.: Xenograft rejection of porcine islet-like cell clusters in normal and natural killer cell-depleted mice. Transplantation 1996, 61, 1313-1320.
• 12. Lambrigts D., Sachs D. H., Cooper D. K. C.: Discordant organ xenotransplantation in primates - world experience and current status. Transplantation 1998, 66, 547-561.
• 13. Mazzoni G., Di Martino C., Demofonti A., Valli A., Pellegrini S., Genili B., Melis M.: Simultaneous allografts of both kidneys in pigs with different portal and caval venous drainage. Am. J. Surg. 1972, 124, 39-42.
• 14. Mazzoni G., Di Martino C., demofonti A., Valli A., Pellegrini S., Gentili B., Melis M.: A comparison of portal and systemic venous drainage in porcine renal allografts. Br. J. Surg. 1972, 59, 541-544.
• 15. Noronha I. L., Oliveira S. G., Tavares T. S., Di Petta A., Dominguez W. V., Perosa M., Genzini T., Romao J. E. Jr., Abensur H., Moura L. A., Filho D. M.: Apoptosis in kidney and pancreas allograft biopsies. Transplantation 2005, 79, 1231-1235.
• 16. Olack B., Jaramillo A., Benshoff N. D., Kaleem Z., Swanson C. J., Lowell J. A., Mohanakumar T.: Rejection of porcine islet xenografts mediated by CD4+ T cells activated through the indirect antigen recognition pathway. Xenotransplantation 2002, 9, 393-401.
• 17. Olack B., Manna P., Jaramillo A., Steward N., Swanson C., Kaesberg K., Poindexter N., Howard T., Mohanakumar T.: Indirect recognition of porcine swine leukocyte Ag class I molecules expressed on islets by human CD4⁺ T lymphocytes. J. Immunol. 2000, 165, 1294-1299.
• 18. Shimizu A., Yamada K., Meehan S. M., Sachs D. H., Colvin R. B.: Acceptance reaction: intragraft events associated with tolerance to renal allografts in miniature swine. J. Am. Soc. Nephrol. 2000, 11, 2371-2380.
• 19. Skuciński J., Nowak W., Wieczorek J., Solecki R.: Chirurgiczne aspekty pobierania nerek u świń dla celów ksenotransplantacji. Medycyna Wet. (w druku).
• 20. Wallgren A. C., Karlsson-Parra A., Korsgren O.: The main infiltrating cell in xenograft rejection is a CD4⁺ macrophage and not a T lymphocyte. Transplantation 1995, 60, 594-601.
• 21. Wang K., Li Y., Jiang H., Shen J., Zhang L.: Pig orthotopic renal allotransplantation model. Transplant Proc. 2003, 35, 191.
• 22. Williams J. M., Holzknecht Z. E., Plummer T. B., Lin S. S., Brun G. J., Platt J. L.: Acute vascular rejection and accommodation: divergent outcomes of the humoral response to organ transplantation. Transplantation 2004, 78, 1471-1478.
• 23. Xu X. C., Naziruddin B., Sasaki H., Smith D. M., Mohanakumar T.: Allele-specific and peptide-dependent recognition of swine leukocyte antigen class I by human cytotoxic T-cell clones. Transplantation 1999, 68, 473-479.
• 24. Yanaga K., Makowka L., Shimada M., Lebeau G., Kahn D., Mieles L. A., Sher L., Chapchap P., Podesta L. G., Starzl T. E.: Improved method of porcine renal allografting for transplantation research. J. Invest. Surg. 1991, 4, 231-236.