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Yellow lupine gene encoding stearoyl-ACP desaturase - organization, expression and potential application

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A gene for the Δ9 desaturase specific to stearoyl-ACP (acyl carrier protein) was iden­tified from yellow lupine (Lupinus luteus) cDNA and genomic libraries through the dif­ferential display method. The desaturase transcript appears in plants infected with Bradyrhizobium sp. (Lupinus) as revealed by Northern hybridization, RT-PCR and ex­pression of β-glucuronidase under the desaturase promoter. A small amount of desaturase transcript was also detected in uninfected plants, which suggests that the gene does not belong to the strict nodule-specific sequences. The desaturase provides unsaturated fatty acids for additional cell membrane synthesis. During nodule and symbiosome development a peribacteroid membrane is formed and the requirement for membrane surface increases, thus the level of desaturase expression is also higher. Transgenic plants of Nicotiana tabacum with overexpression of the full-length lupine stearoyl-ACP desaturase sequence were obtained. They revealed higher con­tent of unsaturated fatty acids (especially oleic acid) in comparison with control plants.
Opis fizyczny
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