Yellow lupine gene encoding stearoyl-ACP desaturase - organization, expression and potential application

• Autorzy: Zaborowska Z. , Starzycki M. , Femiak I. , Swiderski M. , Legocki A.B.
• Języki publikacji: EN

Abstrakty

EN

A gene for the Δ9 desaturase specific to stearoyl-ACP (acyl carrier protein) was iden­tified from yellow lupine (Lupinus luteus) cDNA and genomic libraries through the dif­ferential display method. The desaturase transcript appears in plants infected with Bradyrhizobium sp. (Lupinus) as revealed by Northern hybridization, RT-PCR and ex­pression of β-glucuronidase under the desaturase promoter. A small amount of desaturase transcript was also detected in uninfected plants, which suggests that the gene does not belong to the strict nodule-specific sequences. The desaturase provides unsaturated fatty acids for additional cell membrane synthesis. During nodule and symbiosome development a peribacteroid membrane is formed and the requirement for membrane surface increases, thus the level of desaturase expression is also higher. Transgenic plants of Nicotiana tabacum with overexpression of the full-length lupine stearoyl-ACP desaturase sequence were obtained. They revealed higher con­tent of unsaturated fatty acids (especially oleic acid) in comparison with control plants.

Słowa kluczowe

EN

yellow lupin; gene; nitrogen fixation; Lupinus luteus; gene encoding; Bradyrhizobium; fatty acid; lupin; cDNA; root nodule

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2002
• Tom: 49
• Numer: 1
• Opis fizyczny: p.29-42,fig.

Twórcy

autor

Zaborowska Z.

• Polish Academy of Sciences, Z.Noskowskiego 12-14, 61-704 Poznan, Poland

autor

Starzycki M.

autor

Femiak I.

autor

Swiderski M.

autor

Legocki A.B.

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