Recent progress in oligonucleotide synthesis

• Autorzy: Pfleiderer W. , Matysiak S. , Bergmann F. , Schnell R.
• Języki publikacji: EN

Abstrakty

EN

New blocking group combinations for the machine-aided oligoribonucleotide synthesis on solid phase material have been developed and tested regarding their general application. An acetal function for 2'-OH protection offers a series of advantages in the synthetic approach but special conditions have to be fullfilled in order to guarantee a selective cleavage of the temporary 5'-OH blocking group such as the dansylethoxycarbonyl or even the acid-labile dimethoxytrityl group in the chain elongation process. The final removal of the 2'-0-acetal function in the partially deblocked oligomer proceeds unexpectedly well under weak acidic conditions due to a supposed intramolecular acid catalysis.

Słowa kluczowe

EN

phosphoramidite approach; dansylethoxycarbonyl group; acetal protecting group; solid phase synthesis; 2-[2-nitrophenyl]ethoxycarbonyl group; 2-[4-nitrophenyl]ethyl group; 3-fluoro-4[2-[4-nitrophenyl]ethoxycarbonyloxy]benzyl group; oligonucleotide synthesis

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1996
• Tom: 43
• Numer: 1
• Opis fizyczny: p.37-44

Twórcy

autor

Pfleiderer W.

• Universitat Konstanz, Postfach 5560, D-78434 Konstanz, Germany

autor

Matysiak S.

autor

Bergmann F.

autor

Schnell R.

Bibliografia

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