Over-expression of heat shock factor gene (AtHsfA1d) from Arabidopsis thaliana confers formaldehyde tolerance in tobacco

• Autorzy: Nian H.-J. , Zhang D.-J. , Zeng Z.-D. , Yan J.-P. , Li K.-Z. , Chen L.-M.
• Języki publikacji: EN

Abstrakty

EN

Formaldehyde (HCHO) is a major indoor air pollutant. Plants can be used to remove HCHO from polluted air because they can use HCHO as a carbon source to incorporate it into one-carbon (C1) metabolism. However, high concentrations of exogenous HCHO cause damages to plants. Therefore, genetic engineering is an effective measure to improve ability of plants to clear the HCHO pollution. Expression of AtHsfA1d encoding heat shock transcription factor of Arabidopsis was induced by HCHO stress. AtHsfA1d was cloned into the pYES3 vector and transformed into Saccharomyces cerevisiae. Yeast cells expression of AtHSFA1d showed higher tolerance to HCHO stress than wild-type (WT) cells. AtHsfA1d was introduced into tobacco and the expression of AtHSFA1d in transgenic lines was demonstrated using Western blot analysis. Transgenic tobacco showed higher uptake rate to aqueous HCHO, had the higher biomass and produced higher content of total proteins than WT plants. These results indicated that AtHsfA1d conferred HCHO tolerance to yeast and tobacco. AtHsfA1d is a good candidate to develop phytoremediation plants for HCHO pollution.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2014
• Tom: 36
• Numer: 06
• Opis fizyczny: p.1455-1462,fig.,ref.

Twórcy

autor

Nian H.-J.

• Biotechnology Research Center, Kunming University of Science and Technology, 650500 Kunming, China

autor

Zhang D.-J.

• Biotechnology Research Center, Kunming University of Science and Technology, 650500 Kunming, China

autor

Zeng Z.-D.

• Biotechnology Research Center, Kunming University of Science and Technology, 650500 Kunming, China

autor

Yan J.-P.

• Biotechnology Research Center, Kunming University of Science and Technology, 650500 Kunming, China

autor

Li K.-Z.

• Biotechnology Research Center, Kunming University of Science and Technology, 650500 Kunming, China

autor

Chen L.-M.

• Biotechnology Research Center, Kunming University of Science and Technology, 650500 Kunming, China

Bibliografia

• Achkor H, Diaz M, Fernandez MR, Biosca JA, Pares X, Martinez MC (2003) Enhanced formaldehyde detoxification by overexpression of glutathione-dependent formaldehyde dehydrogenase from Arabidopsis. Plant Physiol 132:2248–2255
• Baniwal SK, Bharti K, Chan KY et al (2004) Heat stress response in plants: a complex game with chaperones and more than twenty heat stress transcription factors. J Biosci 29:471–487
• Banti V, Mafessoni F, Loreti E, Alpi A, Perata P (2010) The heat-inducible transcription factor HsfA2 enhances anoxia tolerance in Arabidopsis. Plant Physiol 152:1471–1483
• Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
• Chen LM, Yurimoto H, Li KZ, Orita I, Akita M, Kato N, Sakai Y, Izui K (2010) Assimilation of formaldehyde in transgenic plants due to the introduction of the bacterial ribulose monophosphate pathway genes. Biosci Biotechnol Biochem 74:627–635
• Döring P, Treuter E, Kistner C, Lyck R, Chen A, Nover L (2000) The role of AHA motifs in the activator function of tomato heat stress transcription factors HsfA1 and HsfA2. Plant Cell 12:265–278
• Gao C, Jiang B, Wang Y, Liu G, Yang C (2012) Overexpression of a heat shock protein (ThHSP18.3) from Tamarix hispida confers stress tolerance to yeast. Mol Biol Rep 39:4889–4897
• Giese M, Bauer-Doranth U, Langebartels C, Sandermann H Jr (1994) Detoxification of formaldehyde by the spider plant (Chlorophytum comosum L.) and by soybean (Glycine max L.) cellsuspension cultures. Plant Physiol 104:1301–1309
• Higashi Y, Ohama N, Ishikawa T, Katori T, Shimura A, Kusakabe K, Yamaguchi-Shinozaki K, Ishida J, Tanaka M, Seki M, Shinozaki K, Sakata Y, Hayashi T, Taji T (2013) HsfA1d, protein identified via FOX hunting using Thellungiella salsuginea cDNAs improves heat tolerance by regulating heat-stress-responsive gene expression. Mol Plant 6:411–422
• Horsch RB, Hoffman NL (1985) A simple and general method for transferring genes into plants. Science 227:1229–1231
• Ito H, Fukuda Y, Murata K, Kimura A (1983) Transformation of intact yeast cells treated with alkali cations. J Bacteriol 15:163–168
• Kotak S, Port M, Ganguli A, Bicker F, Koskull-Döring P (2004) Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization. Plant J 39:98–112
• Lee JH, Hübel A, Schöffl F (1995) Derepression of the activity of genetically engineered heat shock factor causes constitutive synthesis of heat shock proteins and increased thermotolerance in transgenic Arabidopsis. Plant J 8:603–612
• Li HY, Chang CS, Lu LS, Liu CA, Chan MT, Charng YY (2003) Over-expression of Arabidopsis thaliana heat shock factor gene (AtHsfA1b) enhances chilling tolerance in transgenic tomato. Bot Bull Acad Sin 44:129–140
• Li C, Chen Q, Gao X, Qi B, Chen N, Xu S, Chen J, Wang X (2005) AtHsfA2 modulates expression of stress responsive genes and enhances tolerance to heat and oxidative stress in Arabidopsis. Sci China C Life Sci 48:540–550
• Liu ZH, Yang Q, Ma J (2007) A heat shock protein gene (hsp22.4) from Chaetomium globosum confers heat and Na₂CO₃ tolerance to yeast. Appl Microbiol Biotechnol 77:901–908
• Liu AL, Zou J, Liu CF, Zhou XY, Zhang XW, Luo GY, Chen XB (2013) Over-expression of OsHsfA7 enhanced salt and drought tolerance in transgenic rice. BMB Rep 46:31–36
• Murray MG, Thompson WG (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321–4325
• Nian H, Meng Q, Zhang W, Chen L (2013a) Overexpression of the formaldehyde dehydrogenase gene from Brevibacillus brevis to enhance formaldehyde tolerance and detoxification of tobacco. Appl Biochem Biotechnol 169:170–180
• Nian HJ, Meng QC, Cheng Q, Zhang W, Chen LM (2013b) The effects of overexpression of formaldehyde dehydrogenase gene from Brevibacillus brevis on the physiological characteristics of tobacco under formaldehyde stress. Russ J Plant Physl+ 60:764–769
• Nishizawa A, Yabuta Y, Yoshida E, Maruta T, Yoshimura K, Shigeoka S (2006) Arabidopsis heat shock transcription factor A2 as a key regulator in response to several types of environmental stress. Plant J 48:535–547
• Nover L, Bharti K, Döing P, Mishra SK, Ganguli A, Scharf KD (2001) Arabidopsis and the heat stress transcription factor world: how many heat stress transcription factors do we need? Cell Stress Chaperones 6:177–189
• Ogawa D, Yamaguchi K, Nishiuchi T (2007) High-level overexpression of the Arabidopsis HsfA2 gene confers not only increased thermotolerance but also salt/osmotic stress tolerance and enhanced callus growth. J Exp Bot 58:3373–3383
• Schmitz H, Hilgers U, Weidner M (2000) Assimilation and metabolism of formaldehyde by leaves appear unlikely to be of value for indoor air purification. New Phytol 147:307–315
• Shim D, Hwang JU, Lee J, Lee S, Choi Y, An G, Martinoia E, Lee Y (2009) Orthologs of the class A4 heat shock transcription factor HsfA4a confer cadmium tolerance in wheat and rice. Plant Cell 21:4031–4043
• Song ZB, Orita I, Yin F, Yurimoto H, Kato N, Sakai Y, Izui K, Li KZ, Chen LM (2010) Overexpression of an HPS/PHI fusion enzyme from Mycobacterium gastri in chloroplasts of geranium enhances its ability to assimilate and phytoremediate formaldehyde. Biotechnol Lett 32:1541–1548
• Treuter E, Nover L, Ohme K, Scharf K-D (1993) Promoter specificity and deletion analysis of three heat stress transcription factors of tomato. Mol Gen Genet 240:113–125
• Wang SS, Song ZB, Sun Z, Zhang J, Mei Y, Nian HJ, Li KZ, Chen LM (2012) The effects of formaldehyde stress on the physiological characteristics and gene expression associated with photosynthesis in Arabidopsis thaliana. Plant Mol Biol Rep 30:1291–1302
• Wang H, Hu T, Huang J, Lu X, Huang B, Zheng Y (2013) The expression of Millettia pinnata chalcone isomerase in Saccharomyces cerevisiae salt-sensitive mutants enhances salt-tolerance. Int J Mol Sci 14:8775–8786
• Yokotani N, Ichikawa T, Kondou Y, Matsui M, Hirochika H, Iwabuchi M, Oda K (2008) Expression of rice heat stress transcription factor OsHsfA2e enhances tolerance to environmental stresses in transgenic Arabidopsis. Planta 227:957–967
• Yoshida T, Ohama N, Nakajima J et al (2011) Arabidopsis HsfA1 transcription factors function as the main positive regulators in heat shock-responsive gene expression. Mol Genet Genomics 286:321–332

Identyfikatory

DOI

10.1007/s11738-014-1523-y