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2014 | 36 | 06 |

Tytuł artykułu

Over-expression of heat shock factor gene (AtHsfA1d) from Arabidopsis thaliana confers formaldehyde tolerance in tobacco

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Formaldehyde (HCHO) is a major indoor air pollutant. Plants can be used to remove HCHO from polluted air because they can use HCHO as a carbon source to incorporate it into one-carbon (C1) metabolism. However, high concentrations of exogenous HCHO cause damages to plants. Therefore, genetic engineering is an effective measure to improve ability of plants to clear the HCHO pollution. Expression of AtHsfA1d encoding heat shock transcription factor of Arabidopsis was induced by HCHO stress. AtHsfA1d was cloned into the pYES3 vector and transformed into Saccharomyces cerevisiae. Yeast cells expression of AtHSFA1d showed higher tolerance to HCHO stress than wild-type (WT) cells. AtHsfA1d was introduced into tobacco and the expression of AtHSFA1d in transgenic lines was demonstrated using Western blot analysis. Transgenic tobacco showed higher uptake rate to aqueous HCHO, had the higher biomass and produced higher content of total proteins than WT plants. These results indicated that AtHsfA1d conferred HCHO tolerance to yeast and tobacco. AtHsfA1d is a good candidate to develop phytoremediation plants for HCHO pollution.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

36

Numer

06

Opis fizyczny

p.1455-1462,fig.,ref.

Twórcy

autor
  • Biotechnology Research Center, Kunming University of Science and Technology, 650500 Kunming, China
autor
  • Biotechnology Research Center, Kunming University of Science and Technology, 650500 Kunming, China
autor
  • Biotechnology Research Center, Kunming University of Science and Technology, 650500 Kunming, China
autor
  • Biotechnology Research Center, Kunming University of Science and Technology, 650500 Kunming, China
autor
  • Biotechnology Research Center, Kunming University of Science and Technology, 650500 Kunming, China
autor
  • Biotechnology Research Center, Kunming University of Science and Technology, 650500 Kunming, China

Bibliografia

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Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-84cd6c44-5586-4742-9dcc-598573d004b0
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