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2009 | 53 | 3 |

Tytuł artykułu

Application of molecular - biology methods to the diagnosis of botulism in mallard ducks

Autorzy

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The application of molecular-biology methods based on PCR and Real-time PCR in the diagnosis of botulism in mallard ducks was described. The participation of Clostridium botulinum C₁ toxin in the causing of the disease was demonstrated. The results were confirmed by the sequencing of PCR product. The application of molecular-biology methods provides fast and specific analysis of the occurrence and types of Clostridium botulinum toxins.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

53

Numer

3

Opis fizyczny

p.365-368,fig.,ref.

Twórcy

autor
  • Department of Hygiene of Animal Feedingstuffs, National Veterinary Research Institute, 24–100 Pulawy, Poland
autor
  • Department of Hygiene of Animal Feedingstuffs, National Veterinary Research Institute, 24–100 Pulawy, Poland

Bibliografia

  • 1. Cygan Z.M.: Choroby beztlenowcowe zwierząt. Agricultural University, Lublin, 1991, pp. 29-51.
  • 2. Draft: Polymerase chain reaction (PCR) for the detection of botulinum neurotoxin - producing clostridia. CEN/TC 275/WG 6 DOC N368.15th Meeting of CEN/TC 275/WG 6, Helsinki, 2008.
  • 3. Fenicia L., De Medici D., Anniballi F., Delibato E., Aureli P.: Multiplex PCR method to detect botulinum neurotoxins producing clostridia in food and clinical samples. Abstract Book of MED-VET-NET 2nd Annual General Meeting, Malta, 2006.
  • 4. Hatheway C.L.: Botulism: the present status of the disease. CurrTop Microbiol Immunol 1995, 195, 55-75.
  • 5. Jansen B.C.: The toxic antigenic factors produced by Clostridium botulinum types C and D. J Vet Res 1971, 38, 93-98.
  • 6. Lindstrom M., Keto R., Markkula A., Nevas M., Hielm S., Korkeala H.: Multiplex PCR assay for detection and identification of Clostridium botulinum types A, B, E, and F in food and fecal material. Appl Environ Microbiol 2001, 67, 5694-5699.
  • 7. Lindstrom M., Korkeala H.: Laboratory diagnostics of botulism. Clin Microbiol Rev 2006, 19, 298-314.
  • 8. Lindstrom M.K., Jankola H.M., Hyytia E.K., Korkeala H.J.: Identification of Clostridium botulinum with API 20 A, Rapid ID 32 A and RapID ANA II. FEMS 1999, 24, 267-274.
  • 9. Polish Standard: Meat and meat products - Microbiological examinations - Detection of anaerobic spore forming bacteria and anaerobic spore forming bacteria of sulphite reducing. PN - A - 82055 - 12:1997.
  • 10. Polish Standard: Animal feeding stuffs - Requirements and microbiological examinations. PN - R - 64791:1994.
  • 11. Raffestin S., Marvaud J.C., Cerrato R., Dupuy B., Popoff M.R.: Organization and regulation neurotoxin genes in Clostridium botulinum and Clostridium tetani. Anaerobe 2004, 10, 93-100.
  • 12. Raphael B.H., Anreadis J.D.: Real-time PCR detection of the nontoxic nonhemagglutinin gene as a rapid screening method for bacterial isolates harboring the botulinum neurotoxin (A-G) gene complex. J Microbiol Meth 2007, 71, 343-346.
  • 13. Saed E.M.A.: Studies on isolation and identification of Clostridium botulinum investigating field samples specially from equine grass sickness cases. Doctoral dissertation, Faculty of Agriculture, Goettingen University, 2004.
  • 14. Takeshi K, Fujinaga Y., Inoue K., Nakajima H., Oguma K., Ueno T., Sunagawa H., Ohyama T.: Simple method for detection of Clostridium botulinum type A to F neurotoxin genes by polymerase chain reaction. Microbiol Immunol 1996, 40, 5-11.
  • 15. Thomas N.J., Hunter B.D., Athinson C.T.: Infectious Diseases of Wild Birds. Blackwell Publishing, Oxford, 2007, pp. 377-416.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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