The application of molecular-biology methods based on PCR and Real-time PCR in the diagnosis of botulism in mallard ducks was described. The participation of Clostridium botulinum C₁ toxin in the causing of the disease was demonstrated. The results were confirmed by the sequencing of PCR product. The application of molecular-biology methods provides fast and specific analysis of the occurrence and types of Clostridium botulinum toxins.
Department of Hygiene of Animal Feedingstuffs, National Veterinary Research Institute, 24–100 Pulawy, Poland
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