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2012 | 59 | 2 |
Tytuł artykułu

Effect of substrate stiffness on differentiation of umbilical cord stem cells

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Tissue formation and maintenance is regulated by various factors, including biological, physiological and physical signals transmitted between cells as well as originating from cell-substrate interactions. In our study, the osteogenic potential of mesenchymal stromal/stem cells isolated from umbilical cord Wharton's jelly (UC-MSCs) was investigated in relation to the substrate rigidity on polyacrylamide hydrogel (PAAM). Osteogenic differentiation of UC-MSCs was enhanced on stiff substrate compared to soft substrates, illustrating that the mechanical environment can play a role in differentiation of this type of cells. These results show that substrate stiffness can regulate UC-MSCs differentiation, and hence may have significant implications for design of biomaterials with appropriate mechanical properties for regenerative medicine.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
59
Numer
2
Opis fizyczny
p.261-264,fig.,ref.
Twórcy
  • Department of Biophysics and Human Physiology, Centre of Biostructure Research, Medical University of Warsaw, Warszawa, Poland
autor
autor
Bibliografia
  • Anzalone R, Lo Iacono M, Corrao S, Magno F, Loria T, Cappello F, Zummo G, Farina F, La Rocca G (2010) New emerging potentials for human Wharton's jelly mesenchymal stem cells: immunological features and hepatocyte-like differentiative capacity. Stem Cells Dev 19: 423-438. 
  • Anzalone R, Lo Iacono M, Loria T, Di Stefano A, Giannuzzi P, Farina F, La Rocca G (2011) Wharton's jelly mesenchymal stem cells as candidates for beta cells regeneration: extending the differentiative and immunomodulatory benefits of adult mesenchymal stem cells for the treatment of type 1 diabetes. Stem Cell Rev 7: 342-363. 
  • Engler AJ, Richert L, Wong JY, Picart C, Discher DE (2004) Surface probe measurements of the elasticity of sectioned tissue, thin gels and polyelec- trolyte multilayer films: correlations between substrate stiffness and cell adhesion. Surf Sci 570: 142-154.
  • Engler AJ, Sen S, Sweeney HL, Discher DE (2006) Matrix elasticity directs stem cell lineage specification. Cell 126: 677-689. 
  • Evans ND, Minelli C, Gentleman E, LaPointe V, Patankar SN, Kallivretaki M, Chen X, Roberts CJ, Stevens MM (2009) Substrate stiffness affects early differentiation events in embryonic stem cells. Eur Cell Mater 18: 1-14. 
  • Hou T, Xu J, Wu X, Xie Z, Luo F, Zhang Z, Zeng L (2009) Umbilical cord Wharton's Jelly: a new potential cell source of mesenchymal stromal cells for bone tissue engineering. Tissue Eng Part A 15: 2325-2334. 
  • Keogh MB, O'Brien FJ, Daly JS (2010) Substrate stiffness and contractile behaviour modulate the functional maturation of osteoblasts on a collagen-GAG scaffold. Acta Biomater 6: 4305-4313. 
  • Kim K, Yeatts A, Dean D, Fisher JP (2010) Stereolithographic bone scaffold design parameters: osteogenic differentiation and signal expression. Tissue Eng Part B Rev 16: 523-539. 
  • Mahaffy RE, Park S, Gerde E, Kas J, Shih CK (2004) Quantitative analysis of the viscoelastic properties of thin regions of fibroblasts using atomic force microscopy. Biophys J 86: 1777-1793. 
  • McElreavey KD, Irvine AI, Ennis KT, McLean WH (1991) Isolation, culture and characterisation of fibroblast-like cells derived from the Wharton's jelly portion of human umbilical cord. Biochem Soc Trans 19: 29S. 
  • Park JS, Chu JS, Tsou AD, Diop R, Tang Z, Wang A, Li S (2011) The effect of matrix stiffness on the differentiation of mesenchymal stem cells in response to TGF-beta. Biomaterials 32: 3921-3930. 
  • Pelham RJ Jr, Wang Y (1997) Cell locomotion and focal adhesions are regulated by substrate flexibility. Proc Natl Acad Sci USA 94: 13661-13665. 
  • Schneider RK, Puellen A, Kramann R, Raupach K, Bornemann J, Knuechel R, Perez-Bouza A, Neuss S (2010) The osteogenic differentiation of adult bone marrow and perinatal umbilical mesenchymal stem cells and matrix remodelling in three-dimensional collagen scaffolds. Biomaterials 31: 467-480. 
  • Sharma RI, Snedeker J G (2010) Biochemical and biomechanical gradients for directed bone marrow stromal cell differentiation toward tendon and bone. Biomaterials 31: 7695-7704. 
  • Wang HS, Hung SC, Peng ST, Huang CC, Wei HM, Guo YJ, Fu YS, Lai MC, Chen CC (2004) Mesenchymal stem cells in the Wharton's jelly of the human umbilical cord. Stem Cells 22: 1330-1337. 
  • Wang YL, Pelham RJ, Jr (1998) Preparation of a flexible, porous polyacrylamide substrate for mechanical studies of cultured cells. Methods Enzymol 298: 489-496. 
  • Witkowska-Zimny M, Wrobel E (2011) Perinatal sources of mesenchymal stem cells: Wharton's jelly, amnion and chorion. Cell Mol Biol Lett 16: 493-514.
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