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2014 | 36 | 01 |

Tytuł artykułu

Effect of temperature and ripening stages on membrane integrity of fresh-cut tomatoes

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The shelf-life of fresh-cut tomatoes mainly depends on loss of tissue integrity and firmness that occurs also in intact fruits after long-term cold storage due to chilling injury. Round-fruit tomatoes (Solanum lycopersicum L.) cv. Jama were stored in 1.1-L plastic (polyethylene) fresh-cut produce containers as 10.0-mm-thick tomato slices and as intact tomatoes at 4 ± 0.5 °C. The aim of this work was to study the loss of membrane integrity and biochemical processes involved in membrane disruption. Electrolyte leakage and lipid peroxidation were studied at different stages of maturity: mature green, pink (PK), fully ripe and two different storage temperatures: 4 and 15 °C. The tomato slices of PK stage stored at 4 °C did not show changes for both parameters, while significant increase in membrane leakage and lipid peroxidation was observed at 15 °C, especially after 24 h of storage. The enzymes showed a simultaneous increase in their activities with a rise in electrolyte leakage and lipid peroxidation after 7 days of storage. Finally, phospholipase C (PLC) and phospholipase D (PLD) were investigated for intact fruit and tomato slices stored at 4 °C. The PLC had higher activity compared with PLD. In conclusion, the loss of membrane integrity in fresh-cut tomatoes is mainly affected by ripening stages, storage temperature and duration. The wounds enhance the PLC and PLD activities and they play a role late during storage.

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Opis fizyczny



  • Department of Crop Biology, University of Pisa, Vialle delle Piagge 23, 56100 Pisa, Italy
  • ISI Sementi Research stl. Fraz.Ponte Ghiara 8/A, 43036 Fidenza, Italy
  • Institut Cavanilles de Biodiversitat i Biologia Evolutiva, C/Catedratico Jose Beltram 2, 46980 Patema, Valencia, Spain
  • Department of Agricultural and Environmental Sciences, Universita degli Studi di Milano, Via Celoria 2, 20133 Milan, Italy
  • Department of Crop Biology, University of Pisa, Vialle delle Piagge 23, 56100 Pisa, Italy


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