Effect of temperature and ripening stages on membrane integrity of fresh-cut tomatoes

• Autorzy: Natalini A. , Venesa M.-D. , Ferrante A. , Pardossi A.
• Języki publikacji: EN

Abstrakty

EN

The shelf-life of fresh-cut tomatoes mainly depends on loss of tissue integrity and firmness that occurs also in intact fruits after long-term cold storage due to chilling injury. Round-fruit tomatoes (Solanum lycopersicum L.) cv. Jama were stored in 1.1-L plastic (polyethylene) fresh-cut produce containers as 10.0-mm-thick tomato slices and as intact tomatoes at 4 ± 0.5 °C. The aim of this work was to study the loss of membrane integrity and biochemical processes involved in membrane disruption. Electrolyte leakage and lipid peroxidation were studied at different stages of maturity: mature green, pink (PK), fully ripe and two different storage temperatures: 4 and 15 °C. The tomato slices of PK stage stored at 4 °C did not show changes for both parameters, while significant increase in membrane leakage and lipid peroxidation was observed at 15 °C, especially after 24 h of storage. The enzymes showed a simultaneous increase in their activities with a rise in electrolyte leakage and lipid peroxidation after 7 days of storage. Finally, phospholipase C (PLC) and phospholipase D (PLD) were investigated for intact fruit and tomato slices stored at 4 °C. The PLC had higher activity compared with PLD. In conclusion, the loss of membrane integrity in fresh-cut tomatoes is mainly affected by ripening stages, storage temperature and duration. The wounds enhance the PLC and PLD activities and they play a role late during storage.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2014
• Tom: 36
• Numer: 01
• Opis fizyczny: p.191-198,fig.,ref.

Twórcy

autor

Natalini A.

• Department of Crop Biology, University of Pisa, Vialle delle Piagge 23, 56100 Pisa, Italy
• ISI Sementi Research stl. Fraz.Ponte Ghiara 8/A, 43036 Fidenza, Italy

autor

Venesa M.-D.

• Institut Cavanilles de Biodiversitat i Biologia Evolutiva, C/Catedratico Jose Beltram 2, 46980 Patema, Valencia, Spain

autor

Ferrante A.

• Department of Agricultural and Environmental Sciences, Universita degli Studi di Milano, Via Celoria 2, 20133 Milan, Italy

autor

Pardossi A.

• Department of Crop Biology, University of Pisa, Vialle delle Piagge 23, 56100 Pisa, Italy

Bibliografia

• Alferez F, Lluch Y, Burns JK (2008) Phospholipase A2 and postharvest peel pitting in citrus fruit. Postharvest Biol Technol 49:69–76.
• Antonacci S, Natalini A, Cabassi G, Horner D, Ferrante A (2011) Cloning and gene expression analysis of the phospholipase C in wounded spinach leaves during postharvest storage. Postharvest Biol Technol 59:43–52.
• Antunes MDC, Sfakiotakis EM (2008) Changes in fatty acid composition and electrolyte leakage of ‘‘Hayward’’ kiwifruit during storage at different temperatures. Food Chem 110:891–896.
• Artés F, Gómez PA, Artés-Hernández F (2007) Physical, physiological and microbial deterioration of minimally fresh processed fruits and vegetables. Food Sci Technol Int 13:177–188.
• Bergevin M, L’Heureux GP, Thompson JE, Willemot C (1993) Effect of chilling and subsequent storage at 20°C on electrolyte leakage and phospholipid fatty acid composition of tomato pericarp. Physiol Plant 87:522–527.
• Boukobza F, Dunphy PJ, Taylor AJ (2001) Measurement of lipid oxidation-derived volatiles in fresh tomatoes. Postharvest Biol Technol 23:117–131.
• Brecht JK, Saltveit ME, Talcott ST, Bartz JA (2004) Fresh-cut vegetables and fruits horticultural review 30: 185–250.
• Brummell DA, Harpster MH (2001) Cell wall metabolism in fruit softening and quality and its manipulation in transgenic plants. Plant Mol Biol 47:311–340.
• Brummell DA, Harpster MH, Civello PM, Palys JM, Bennett AB, Dunsmuir P (1999) Modification of expansin protein abundance in tomato fruit alters softening and cell wall polymer metabolism during ripening. Plant Cell 11:2203–2216.
• Ferrante A, Martinetti L, Maggiore T (2009) Biochemical changes in cut vs. intact lamb’s lettuce (Valerianella olitoria) leaves during storage. Int J Food Sci Technol 44:1050–1056.
• Francis GA, Gallone Nychas GJ, Sofos JN, Colelli G, Amodio ML, Spano G (2012) Factors affecting quality and safety of fresh-cut produce. Crit Rev Food Sci Nutr 52(7):595–610.
• Gorny JR, Cifuentes RA, Hess-Pierce B, Kader AA (2000) Quality changes in fresh-cut pear slices as affected by cultivar, ripeness stage, fruit size, and storage regime. J Food Sci 65:541–544.
• Gupta MN, Wold F (1980) A convenient spectrophotometric assay for phospholipase D using D-nitrophenyl-phosphocholine as substrate. Lipids 15:594–596.
• Hakim A, Austin ME, Batal D, Gullo S, Khatoon M (2004) Quality of fresh-cut tomatoes. J Food Qual 27(3):195–206.
• Health RL, Packer L (1968) Photoperoxidation in isolated chloroplasts kinetic and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:180–198.
• Hodges DM, Toivonen PMA (2008) Quality of fresh-cut fruits and vegetables as affected by exposure to abiotic stress. Postharvest Biol Technol 48:155–162.
• Hong JH, Gross KC (2001) Maintaining quality of fresh-cut tomato slice through modified atmosphere packaging and low temperature storage. J Food Sci 66:960–965.
• Huber DJ, Karakurt Y, Jeong J (2001) Pectin degradation in ripening and wounded fruits. Revista Brasileira de Fisiologia Vegetale 13:224–241.
• Jeong J, Brecht JK, Huber DJ, Sargent SA (2004) 1-Methylcyclopropene (1-MCP) for maintaining texture quality of fresh-cut tomato. HortScience 39:1359–1362.
• Juan M, Rivero RM, Romero L, Ruiz J (2005) Evaluation of some nutritional and biochemical indicators in selecting salt-resistant tomato cultivars. Environ Exp Bot 54:193–201.
• Karakurt Y, Huber DJ (2003) Activities of several membrane and cell-wall hydrolases, ethylene byosinthetic enzymes, and cell wall polyuronide degradation during low temperature storage of intact and fresh-cut papaya (Carica papaya) fruit. Postharvest Biol Technol 28:219–229.
• Kurioka S, Matsuda M (1976) Phospholipase C assay using Dnitrophenylphosphorylcholine together with sorbitol and its application to studying the metal and detergent requirement of the enzyme. Anal Biochem 75:281–289.
• Lana MM, Tijskens LMM (2006) Effects of cutting and maturity on antioxidant activity of fresh-cut tomatoes. Food Chem 97: 203–211.
• Lana MM, Tijskens LMM, van Kooten O (2005) Effects of storage temperature and fruit ripening on firmness of fresh-cut tomatoes. Postharvest Biol Technol 35:87–95.
• Lana MM, Tijskens LMM, van Kooten O (2006) Modelling RGB colour aspects and translucency of fresh-cut tomatoes. Postharvest Biol Technol 40:15–25.
• Lurie S, Laamin M, Lapsker Z, Fallik E (1997) Heat treatments to decrease chilling injury in tomato fruit: effects on lipids, pericarp lesions and fungal growth. Physiol Plant 90:140–145.
• Magee RI, Caporaso E, Prakash A (2003) Effects of exogenous calcium salt treatments on inhibiting irradiation-induced softening in diced Roma tomatoes. J Food Sci 2430–2435.
• Mao L, Karakurt Y, Huber DJ (2004) Incidence of water-soaking and phospholipid catabolism in ripe watermelon (Citrullus lanatus) fruit: induction by ethylene and prophylactic effects of 1-methylcyclopropene. Postharvest Biol Technol 33:1–9.
• Marangoni AG, Palma T, Stanley DW (1996) Membrane effects in postharvest physiology. Postharvest Biol Technol 7:193–217.
• Natalini A, Schouten RE, Woltering EJ (2010) The locular gel differentially affect translucency development and firmness decay in fresh-cut tomato slices. Postharvest Biol Technol 58:72–77.
• Russo M, Sgherri C, Izzo R, Navari-Izzo F (2008) Brassica napus subjected to copper excess: phospholipases C and D and glutathione system in signalling. Environ Exp Bot 62:238–246.
• Saladié M, Matas AJ, Isaacson T, Jenks MA, Goodwin SM, Niklas KJ, Xiaolin R, Labavitch JM, Shackel KA, Fernie AR, Lytovchenko A, O’Neill MA, Watkins CB, Rose JKC (2007) A re-evaluation of the key factors that contribute to tomato fruit softening and integrity. Plant Physiol 144:1012–1028.
• Saltveit M (2002) The rate of ion leakage from chilling-sensitive tissue does not immediately increase upon exposure to chilling temperatures. Postharvest Biol Technol 26:295–304.
• Saltveit M (2005) Influence of heat shocks on the kinetics of chillinginduced ion leakage from tomato pericarp discs. Postharvest Biol Technol 36:87–92.
• Sargent SA, Moretti CL (2002) Tomato. In: Gross, KC, Wang CY, Saltveit ME (eds.) Agricultural Handbook 66: the commercial storage of fruits, vegetables and florist and nursery crops.
• Schouten RE, Natalini A, Tijskens LMM, Woltering EJ, van Kooten O (2010) Modelling the firmness behaviour of cut tomatoes. Postharvest Biol Technol 57:44–51.
• Sharom M, Willemot C, Thompson JE (1994) Chilling injury indices lipid phase changes in membranes of tomato fruit. Plant Physiol 105:305–308.
• Soliva-Fortuny RC, Martin-Belloso O (2003) New advances in extending the shelf-life of fresh-cut fruits: a review. Trends Food Sci Technol 14:341–353.
• Soliva-Fortuny RC, Grigelmo MN, Hernando J, Lluch MA, Martin Belloso O (2002) Effect of minimal processing on the textural properties of fresh-cut pears. J Food Agric 82:1682–1688.
• Tu Chung T, West G, Tucker GA (2006) Effect of wounding on cell wall hydrolase activity in tomato fruit. Postharvest Biol Technol 40:250–255.
• Wang C, Zien CA, Afthile M, Welti R, Hildebrand DF, Wang X (2000) Involvement of phospholipase D in wound-induced accumulation of jasmonic acid in Arabidopsis. Plant Cell 12:2237–2246.

Identyfikatory

DOI

10.1007/s11738-013-1399-2