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2018 | 71 | 4 |
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Screening of the genotypes of bread wheat (Triticum aestivum L.) by the allelic variants of Waxy genes and HMW glutenin subunits

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Warianty tytułu
PL
Porównanie genotypów pszenicy pszennej (Triticum aestivum L.) pod względem występowania wariantów allelicznych genów Waxy i podjednostek gluteninowych HMW
Języki publikacji
EN
Abstrakty
EN
Screening genotypes of wheat by allelic variants of Waxy and HMW-GS genes is a constitutional unit of marker-assisted selection of varieties with high values of desirable properties for flour-baking and technological attributes of the grain. The aim of the study was to produce a molecular screening of samples of spring soft wheat of local selection lines for the detection of genotypes of Triticum aestivum L. with valuable alleles of Waxy and HMW-GS genes. Seventy samples of wheat were subjected to DNA testing for the identification of genotypes with alleles of Waxy and HMW-GS genes. Molecular screening of wheat samples with the selected systems of molecular marking of the allelic variants of the analyzed genes made it possible to detect three partially Waxy lines with a combination of two null-alleles (Wx-A1b and Wx-B1b) in the study collection, as well as 33 plants with an economically-valuable combination of Ax2*/5+10 subunits of HMW-GS, the genotypes of which are considered as starting material for further selection work on the creation of spring soft wheat cultivars with high quality indicators of grain. The approaches applied in the study to identify Waxy and HMW-GS alleles are effective methods of evaluating their allelic polymorphism.
PL
Analiza genotypów pszenicy pod względem występowania wariantów allelicznych genów Waxy i HMW-GS jest kluczowa dla selekcji odmian o pożądanych wartościach wypiekowych i parametrach technologicznych ziarna. Celem badań była analiza molekularna 70 lokalnych linii jarej pszenicy zwyczajnej w celu wykrycia genotypów Triticum aestivum L. z allelami genów Waxy i HMW-GS. Badanie prób pszenicy za pomocą systemu selekcji wspomaganej markerami molekularnymi dla wariantów allelicznych analizowanych genów pozwoliło na wykrycie trzech linii z kombinacją dwóch niemych alleli (Wx-A1b i Wx-B1b), a także 33 roślin o ekonomicznie istotnej kombinacji podjednostek Ax2*/5+10 HMW-GS, których genotypy są uważane za materiał wyjściowy do dalszych prac selekcyjnych nad tworzeniem odmian jarej pszenicy zwyczajnej o wysokiej jakości parametrów ziarna. Zastosowane w badaniach podejście do identyfikacji alleli Waxy i HMW-GS może być skuteczną metodą do oceny ich polimorfizmu allelicznego.
Słowa kluczowe
Wydawca
-
Czasopismo
Rocznik
Tom
71
Numer
4
Opis fizyczny
Article: 1746 [10 p.], fig.,ref.
Twórcy
autor
  • Interdisciplinary Research and Technology Center of Food Quality Monitoring, All-Russian Scientific Research Institute of the Brewing, Non-Alcoholic and Wine Industry – Branch of the V. M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences, 7 Rossolimo, Moscow 119021, Russian Feder
autor
  • Laboratory of the Selection of Bread Wheat, Tatar Scientific Research Institute of Agriculture – Autonomous Subdivision of Federal Research Center, Kazan Scientific Center of RAS, 48 Orenburgskiy tract, Kazan 420059, Republic of Tatarstan, Russian Federation
  • Laboratory of the Selection of Bread Wheat, Tatar Scientific Research Institute of Agriculture – Autonomous Subdivision of Federal Research Center, Kazan Scientific Center of RAS, 48 Orenburgskiy tract, Kazan 420059, Republic of Tatarstan, Russian Federation
  • Laboratory of the Selection of Bread Wheat, Tatar Scientific Research Institute of Agriculture – Autonomous Subdivision of Federal Research Center, Kazan Scientific Center of RAS, 48 Orenburgskiy tract, Kazan 420059, Republic of Tatarstan, Russian Federation
autor
  • Laboratory of the Selection of Bread Wheat, Tatar Scientific Research Institute of Agriculture – Autonomous Subdivision of Federal Research Center, Kazan Scientific Center of RAS, 48 Orenburgskiy tract, Kazan 420059, Republic of Tatarstan, Russian Federation
Bibliografia
  • Vanzetti LS, Pfluger LA, Rodriguez-Quijano M, Carrillo JM, Helguera M. Genetic variability for waxy genes in Argentinean bread wheat germplasm. Electronic Journal of Biotechnology. 2009;12(1). https://doi.org/10.2225/vol12-issue1-fulltext-2
  • Klimushina MV, Gladkih NI, Divashuk MG, Bespalova LA, Vasilyev AV, Karlov GI. Distribution of allelic variants of Wx genes in the common wheat collection made at the Krasnodar Lukyanenko Research Institute of Agriculture. Vavilov Journal of Genetics and Breeding. 2012;16(1):187–192.
  • Guzmán C, Ortega R, Yamamori M, Pena RJ, Alvarez JB. Molecular characterization of two novel null waxy alleles in Mexican bread wheat landraces. J Cereal Sci. 2015;62:8–14. https://doi.org/10.1016/j.jcs.2014.11.003
  • Kocourkova Z, Bradova J, Kohutova Z, Slamova L, Vejl P, Horcicka P. Wheat breeding for the improved bread-making quality using PCR based markers of glutenins. Czech J Genet Plant Breed. 2008;44(3):105–113. https://doi.org/10.17221/20/2008-CJGPB
  • Chnapek M, Peroutkova R, Vivodik M, Galova Z. Identification of technologically important genes and their products in the collection of bread wheat genotypes. J Microbiol Biotechnol Food Sci. 2015;4(2):26–29. https://doi.org/10.15414/jmbfs.2015.4.special2.26-29
  • Janni M, Cadonici S, Pignone D, Marmiroli N. Survey and new insights in the application of PCR‐based molecular markers for identification of HMW‐GS at the Glu‐B1 locus in durum and bread wheat. Plant Breed. 2017;136(4):467–473. https://doi.org/10.1111/pbr.12506
  • Yamamori M, Quynh NT. Differential effects of Wx-A1, -B1 and -D1 protein deficiencies on apparent amylase content and starch pasting properties in common wheat. Theor Appl Genet. 2000;100:32–38. https://doi.org/10.1007/s001220050005
  • Guzmán C, Alvarez JB. Wheat waxy proteins: polymorphism, molecular characterization and effects on starch properties. Theor Appl Genet. 2016;129(1):1–16. https://doi.org/10.1007/s00122-015-2595-9
  • Caramanico R, Barbiroli Marengo AM, Fessas D, Bonomi F, Lucisano M, Pagani MA, et al. Interplay between starch and proteins in waxy wheat. Cereal Science. 2017;75:198–204. https://doi.org/10.1016/j.jcs.2017.04.008
  • Liu S, Chao S, Anderson JA. New DNA markers for high molecular weight glutenin subunits in wheat. Theor Appl Genet. 2008;118(1):177–183. https://doi.org/10.1007/s00122-008-0886-0
  • Ayala M, Alvarez JB, Yamamori M, Guzmán C. Molecular characterization of waxy alleles in three subspecies of hexaploid wheat and identification of two novel Wx-B1 alleles. Theor Appl Genet. 2015;128(12):2427–2435. https://doi.org/10.1007/s00122-015-2597-7
  • McLauchlan A, Ogbonnaya FC, Hollingsworth B, Carter M, Gale KR, Henry RJ, et al. Development of robust PCR-based DNA markers for each homeo-allele of granule-bond starch synthase and their application in wheat breeding programs. Australian Journal of Agriculture Research. 2001;52(11–12):1409–1416. https://doi.org/10.1071/AR01036
  • Nakamura T, Vrinten P, Saito M, Konda M. Rapid classification of partial waxy wheats using PCR-based markers. Genome. 2002;45:1150–1156. https://doi.org/10.1139/g02-090
  • Goutam U, Tiwari R, Gupta RK, Kukreja S, Chaudhury A. Allelic variations of functional markers for high molecular weight glutenin genes in Indian wheat (Triticum aestivum L.) cultivars and their correlation with bread loaf volume. Indian J Plant Physiol. 2015;20(1):97–102. https://doi.org/10.1007/s40502-015-0141-z
  • Guzmán C, Caballero L, Alvarez JB. Molecular characterisation of the Wx-B1 allelic variants identified in cultivated emmer wheat and comparison with those of durum wheat. Mol Breeding. 2011;28(3):403–411. https://doi.org/10.1007/s11032-010-9493-2
  • Guzmán C, Alvarez JB. Molecular characterization of a novel waxy allele (Wx-Au1a) from Triticum urartu Thum. ex Gandil. Genet Resour Crop Evol. 2012;59:971–979. https://doi.org/10.1007/s10722-012-9849-z
  • Guzmán C, Caballero L, Martin LM, Alvarez JB. Waxy genes from spelt wheat: new alleles for modern wheat breeding and new phylogenetic inferences about the origin of this species. Ann Bot. 2012;110(6):1161–1171. https://doi.org/10.1093/aob/mcs201
  • Vafin RR, Abdulina IR, Rzhanova IV, Garaeva AL, Tyulkin SV, Askhadullin DF, et al. Molecular approaches for identification of Tritictum aestivum L. genotypes by allelic variants of Waxy-genes and HMW glutenin subunits. Modern Problems of Science and Education. 2015;2015(2):[13 p.].
  • Abdulina IR, Vafin RR, Tyulkin SV, Zaynullin LI, Alimova FK, Askhadullin DF, et al. Detection of null Wx-B1b-allele of the Waxy-gene in spring wheat genotypes of Russian breeding. Modern Problems of Science and Education. 2013;2013(2):[7 p.].
  • Lafiandra D, Tucci GF, Pavoni A, Turhcetta T, Margiotta B. PCR analysis of x- and y-type genes present at the complex Glu-A1 locus in durum and bread wheat. Theor Appl Genet. 1997;94(2):235–240. https://doi.org/10.1007/s001220050405
  • Yi X, Jiang Z, Hu W, Zhao Y, Bie T, Gao D, et al. Development of a competitive allele‐specific PCR marker for selection of the mutated Wx‐D1d allele in wheat breeding. Plant Breed. 2017;136(4):460–466. https://doi.org/10.1111/pbr.12504
  • Yasui T, Sasaki T, Matsuki J. Waxy bread wheat mutants, K107Wx1 and K107Wx2, have a new null allele on Wx-D1 locus. Breed Sci. 1998;48(4):405–407. https://doi.org/10.1270/jsbbs1951.48.405
  • Moiseenko LM, Klykov AG, Konovalova IV, Bogdan PM. Ispolzovanie ozimykh sortov v selektsii yarovoi myagkoi pshenitsy s tseliyu povysheniya geneticheskogo potentsiala produktivnosti. Dostizheniya Nauki i Tekhniki APK. 2011;12:28–30.
  • Vafin RR, Rzhanova IV, Askhadullin DF, Askhadullin DF, Vasilova NZ, Tagirov MS, et al. Identification of Triticum aestivum L. genotype by allelic versions of Waxy-genes and HMW glutenin subunits. Ecology, Environment and Conservation. 2015;21:137–143.
  • Zhang LL, Chen H, Luo M, Zhang XW, Deng M, Ma J, et al. Transposon insertion resulted in the silencing of Wx-B1n in Chinese wheat landraces. Theor Appl Genet. 2017;130(6):1321–1330. https://doi.org/10.1007/s00122-017-2878-4
Typ dokumentu
Bibliografia
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