Screening of the genotypes of bread wheat (Triticum aestivum L.) by the allelic variants of Waxy genes and HMW glutenin subunits

• Autorzy: Vafin R. , Rzhanova I. , Askhadullin D. , Askhadullin D. , Vasilova N.

Warianty tytułu

PL

Porównanie genotypów pszenicy pszennej (Triticum aestivum L.) pod względem występowania wariantów allelicznych genów Waxy i podjednostek gluteninowych HMW

• Języki publikacji: EN

Abstrakty

EN

Screening genotypes of wheat by allelic variants of Waxy and HMW-GS genes is a constitutional unit of marker-assisted selection of varieties with high values of desirable properties for flour-baking and technological attributes of the grain. The aim of the study was to produce a molecular screening of samples of spring soft wheat of local selection lines for the detection of genotypes of Triticum aestivum L. with valuable alleles of Waxy and HMW-GS genes. Seventy samples of wheat were subjected to DNA testing for the identification of genotypes with alleles of Waxy and HMW-GS genes. Molecular screening of wheat samples with the selected systems of molecular marking of the allelic variants of the analyzed genes made it possible to detect three partially Waxy lines with a combination of two null-alleles (Wx-A1b and Wx-B1b) in the study collection, as well as 33 plants with an economically-valuable combination of Ax2*/5+10 subunits of HMW-GS, the genotypes of which are considered as starting material for further selection work on the creation of spring soft wheat cultivars with high quality indicators of grain. The approaches applied in the study to identify Waxy and HMW-GS alleles are effective methods of evaluating their allelic polymorphism.

PL

Analiza genotypów pszenicy pod względem występowania wariantów allelicznych genów Waxy i HMW-GS jest kluczowa dla selekcji odmian o pożądanych wartościach wypiekowych i parametrach technologicznych ziarna. Celem badań była analiza molekularna 70 lokalnych linii jarej pszenicy zwyczajnej w celu wykrycia genotypów Triticum aestivum L. z allelami genów Waxy i HMW-GS. Badanie prób pszenicy za pomocą systemu selekcji wspomaganej markerami molekularnymi dla wariantów allelicznych analizowanych genów pozwoliło na wykrycie trzech linii z kombinacją dwóch niemych alleli (Wx-A1b i Wx-B1b), a także 33 roślin o ekonomicznie istotnej kombinacji podjednostek Ax2*/5+10 HMW-GS, których genotypy są uważane za materiał wyjściowy do dalszych prac selekcyjnych nad tworzeniem odmian jarej pszenicy zwyczajnej o wysokiej jakości parametrów ziarna. Zastosowane w badaniach podejście do identyfikacji alleli Waxy i HMW-GS może być skuteczną metodą do oceny ich polimorfizmu allelicznego.

• Wydawca: -
• Czasopismo: Acta Agrobotanica
• Rocznik: 2018
• Tom: 71
• Numer: 4
• Opis fizyczny: Article: 1746 [10 p.], fig.,ref.

Twórcy

autor

Vafin R.

• Interdisciplinary Research and Technology Center of Food Quality Monitoring, All-Russian Scientific Research Institute of the Brewing, Non-Alcoholic and Wine Industry – Branch of the V. M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences, 7 Rossolimo, Moscow 119021, Russian Feder

autor

Rzhanova I.

• Laboratory of the Selection of Bread Wheat, Tatar Scientific Research Institute of Agriculture – Autonomous Subdivision of Federal Research Center, Kazan Scientific Center of RAS, 48 Orenburgskiy tract, Kazan 420059, Republic of Tatarstan, Russian Federation

autor

Askhadullin D.

• Laboratory of the Selection of Bread Wheat, Tatar Scientific Research Institute of Agriculture – Autonomous Subdivision of Federal Research Center, Kazan Scientific Center of RAS, 48 Orenburgskiy tract, Kazan 420059, Republic of Tatarstan, Russian Federation

autor

Askhadullin D.

• Laboratory of the Selection of Bread Wheat, Tatar Scientific Research Institute of Agriculture – Autonomous Subdivision of Federal Research Center, Kazan Scientific Center of RAS, 48 Orenburgskiy tract, Kazan 420059, Republic of Tatarstan, Russian Federation

autor

Vasilova N.

• Laboratory of the Selection of Bread Wheat, Tatar Scientific Research Institute of Agriculture – Autonomous Subdivision of Federal Research Center, Kazan Scientific Center of RAS, 48 Orenburgskiy tract, Kazan 420059, Republic of Tatarstan, Russian Federation

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Identyfikatory

DOI

10.5586/aa.1746