Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników


2015 | 61 | 2 |

Tytuł artykułu

Study of antiurolithiatic activity of a formulated herbal suspension


Treść / Zawartość

Warianty tytułu

Badania nad działaniem mieszanki ziołowej w kamicy nerkowej

Języki publikacji



Urolithiasis is the process of formation of stone in the urinary tract by crystal nucleation, aggregation and retention in the urinary tract. Traditional medicinal plants have been successfully used to overcome urolithiasis. Hence, herbal formulation containing a mixture of plant extracts was prepared and evaluated for the antiurolithiatic activity. This formulation contained alcoholic extracts of fruit of Tribulus terrestris, root of Boerhavia diffusa and leaves of Azadirachta indica Studies were performed in ethylene glycol-induced urolithiasis model using Cystone as a standard drug. Ethylene glycol increases the level of calcium, oxalate and phosphate which are responsible for urolithiasis. The herbal suspension decreased the level of calcium, oxalate and phosphate significantly at doses of 200 and 300 mg/kg when compared to the negative control group. Creatine, uric acid and urea were also decreased significantly at all dose levels. Histopathology has supported these results. The level of LD50 was found to be higher than 2000 mg/kg. Therefore, the prepared formulation has appreciable significant antiurolithiatic activity and is safe for use.
Kamica nerkowa to proces polegający na tworzeniu, agregacji i odkładaniu się kryształów w układzie moczowym. Do przeciwdziałania kamicy nerkowej z powodzeniem stosuje się tradycyjne zioła. W przedstawionej pracy przygotowano mieszankę ziołową zawierającą wyciągi roślinne i zbadano jej właściwości hamujące rozwój kamicy. W skład mieszanki wchodziły wyciągi alkoholowe z owoców Tribulus terrestris, korzeni Boerhavia diffusa oraz liści Azadirachta indica. Badanie przeprowadzono na modelu kamicy nerkowej indukowanej glikolem etylenowym, stosując jako kontrolę standardowy lek Cystone. Glikol etylenowy podnosi poziom wapnia, szczawianów i fosforanów, które są odpowiedzialne za rozwój kamicy. Zastosowanie mieszanki ziołowej (w dawce 200 i 300 mg/kg) istotnie obniżyło poziom wapnia, szczawianów i fosforanów w porównaniu z negatywną grupą kontrolną. Wszystkie dawki obniżały też istotnie poziom kreatyny, kwasu moczowego i mocznika. Uzyskane wyniki zostały potwierdzone przez badanie histopatologiczne. Dawka LD50 była wyższa niż 2000 mg/kg, przygotowana mieszanka ma zatem silne działanie przeciwkamicowe i jest bezpieczna w użyciu.









Opis fizyczny



  • Rajeev Gandhi College of Pharmacy, Bhopal, Madhya Pradesh, India-462042
  • Rajeev Gandhi College of Pharmacy, Bhopal, Madhya Pradesh, India-462042


  • 1. Ashok P, Koti BC, Vishwanathswamy AH. Antiurolithiatic and antioxidant activity of Mimusops elengi on ethylene glycol-induced urolithiasis in rats. Indian J Pharmacol 2010; 42(6):380-383.
  • 2. Coe FL, Evan A, Worcester E. Kidney stone disease. J Clin Invest 2005; 115(10): 2598-2608.
  • 3. Atodariya U, Barad R, Upadhyay S, Upadhyay U. Anti-urolithiatic activity of Dolichos biflorus seeds. J Pharmacogn Phytochem 2013; 2(2):209-213.
  • 4. Pathak AK, Argal A. Analgesic activity of Calotropis gigantea flower. Fitoterapia 2007; 78:40-42.
  • 5. yadav RD, Alok S, Jain SK, Verma A, Mahor A, Bharti JP et al. Herbal plants used in the treatment of urolithiasis: a review. Int J Pharmaceutical Sci Res 2011; 2(6):1412-1420.
  • 6. Narayana SV, Ali VS. Pashanabheda. J Res Indian Med 1967; 1:24.
  • 7. Baheti DG, Kadam SS. Antiurolithiatic activity of a polyherbal formulation against calcium oxalate induced urolithiasis in rats. JAPER 2013; 3:61-71.
  • 8. Makasana A, Ranpariya V, Desai D, Mendpara J, Parekh V. Evaluation for the anti-urolithiatic activity of Launaea procumbens against ethylene glycol-induced renal calculi in rats. Toxicol Rep 2014; 1:46-52.
  • 9. Prasad K, Sujatha D, Bharathi K. Herbal drugs in urolithiasis - a review. Pharmacog Rev 2007; 1:175-179.
  • 10. Nagal A, Singla RK. Herbal resources with antiurolithiatic effects: A Review. Indo Global J Pharm Sci 2013; 3(1):6-14.
  • 11. Kishore RN, Mangilal T, Anjaneyulu N, Abhinayani G, Sravya N. Investigation of anti-urolithiatic activity of Brassica oleracea gongylodes and Desmostachya bipinnata in experimentally induced urolithiasis in animal models. Int J Pharm Pharm Sci 2014; 6(6):602-604.
  • 12. Khan A, Khan SR, Gilani AH. Studies on the in vitro and in vivo antiurolithic activity of Holarrhena antidysenterica. Urol Res 2012; 40(6):671-681.
  • 13. Gupta SK, Baghel MS, Bhuyan C, Ravishankar B, Ashok BK, Patil PD. Evaluation of anti-urolithiatic activity of Pashanabhedadi Ghrita against experimentally induced renal calculi in rats. Ayu 2012; 33(3):429-434.
  • 14. Al-Bayati FA, Al-Mola HF. Antibacterial and antifungal activities of different parts of Tribulus terrestris L. growing in Iraq. J Zhejiang University Science B. 2008; 9(2):154-159.
  • 15. Chhatre S, Nesari T, Somani G, Kanchan D, Sathaye S. Phytopharmacological overview of Tribulus terrestris Phcog Rev 2014; 8:45-51.
  • 16. Sangeeta D, Sidhu H, Thind SK, Nath R. Effect of Tribulus terrestris on oxalate metabolism in rats. J Ethnopharmacol 1994; 44:61-66.
  • 17. Tilwari A, Shukla NP, Uma Devi P. Effect of five medicinal plants used in Indian system of medicines on immune function in Wistar rats. Afr J Biotechnol 2011; 10:16637-16645.
  • 18. Heidari MR, Mehrabani M, Pardakhty A, Khazaeli P, Zahedi MJ, yakhchali M et al. The analgesic effect of Tribulus terrestris extract and comparison of gastric ulcerogenicity of the extract with indomethacine in animal experiments. Ann N y Acad Sci 2007; 1095:418-427.
  • 19. Baburao B, Rajyalakshmi G, Venkatesham A, Kiran G, Shyamsunder A, Gangarao B. Anti-inflammatory and antimicrobial activities of methanolic extract of Tribulus terrestris Linn plant. Int J Chem Sci 2009; 7:1867-1872.
  • 20. Arcasoy HB, Erenmemisoglu A, Tekol y, Kurucu S, Kartal M. Effect of Tribulus terrestris L saponin mixture on some smooth muscle preparations: a preliminary study. Boll Chim Farm 1998; 137:473-475.
  • 21. Hiruma-Lima CA, Gracioso JS, Bighetti EJB, Robineou GL, Souza Brito ARM. The juice of fresh leaves of Boerhaavia diffusa L. (Nyctaginaceae) markedly reduces pain in mice. J Ethnopharmacol 2000; 71:267-274.
  • 22. Girish HV, Satish S. Antibacterial activity of important medicinal plants on human pathogenic bacteria - a comparative analysis. World Appl Sci J 2008; 5(3):267-271.
  • 23. Chauhan CK, Joshi MJ, Vaidya AD. Growth inhibition of struvite crystals in the presence of herbal extract Commiphora wightii. J Mater Sci Mater Med 2009; 20(1):S85-92.
  • 24. Biswas K, Chattopadhyay A, Banerjee RK, Bandyopadhyay U. Biological activities and medicinal properties of neem (Azadirachta indica). Curr Sci 2002; 82(11):1336-1345.
  • 25. Maragathavalli S, Brindha S, Kaviyarasi NS, Annadurai BB, Gangwar SK. Antimicrobial activity in leaf extract of neem (Azadirachta indica Linn.). Intern J Sci Nat 2012; 3(1):110.
  • 26. Hwisa NT, Assaleh FH, Gindi S, Melad FE, Chandu BR, Katakam P. A study on antiurolithiatic activity of Melia azadirachta L. aqueous extract in rats. Am J Pharm Sci 2014; 2(1):27-31.
  • 27. Atmani F, Slimani y, Mimouni M, Hacht B. Prophylaxis of calcium oxalate stones by Herniaria hirsuta on experimentally induced nephrolithiasis in rats. BJU Inter 2003; 92:137-40.
  • 28. Mitra SK, Gopumadhavan S, Venkataranganna MV, Sundaram R. Effect of Cystone, a herbal formulation, on glycolic acid-induced urolithiasis. Phytother Res 1998; 12:372-374.
  • 29. Medeiros DM, Mustafa MA. Proximate composition, mineral content and fatty acids of cat fish (Ictalurus punctatus Rafinesque) for different seasons and cooking methods. J Food Sci 1985; 50:585-587.
  • 30. Hodgkinson A, Williams A. An improved colorimetric procedure for urine oxalate. Clin Chim Acta 1972; 36:127-132.
  • 31. Fiske CH, Subbarow y. The colorimetric determination of phosphate. J Biol Chem 1925; 66:375-400.
  • 32. Caraway WT. Uric acid. In: Seligson D (ed). Standard methods of clinical chemistry. 4th ed. London Academic Press 1963:239-247.
  • 33. Michell AR. Urolithiasis-historical, comparative and pathophysiological aspects: a review. J R Soc Med 1989; 82:669-672.
  • 34. Karadi RV, Gadge NB, Alagawadi KR, Savadi RV. Effect of Moringa oleifera Lam. root-wood on ethylene glycol induced urolithiasis in rats. J Ethnopharmacol 2006; 105: 306-311.
  • 35. Soundararajan P, Mahesh R, Ramesh T, Begum VH. Effect of Aerva lanata on calcium oxalate urolithiasis in rats. Indian J Exp Bio 2006; 44:981-986.
  • 36. Talekar yP, Gund KA, Kale SD, Apte KG, Parab PB. Antiurolithic activity of corn silk extract in rats. Int J Univers Pharm Life Sci 2013; 2(4):65-77.
  • 37. Aggarwal A, Tandon S, Singla SK, Tandon C. A novel antilithiatic protein from Tribulus terrestris having cytoprotective potency. Protein Pept Lett 2012; 19:812-819.

Typ dokumentu



Identyfikator YADDA

JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.