PL
Badaniami objęto lipopolisacharydy wyekstrahowane z dziewięciu klinicznych szczepów B. fragilis wyizolowanych w Polsce. Biologiczną aktywność preparatów LPS oznaczono przy użyciu fotometrycznego testu BET (poprzednio LAL). Aktywność lipopolisacharydów klinicznych szczepów B. fragilis porównano z aktywnością LPS referencyjnych szczepów B. fragilis i LPS E. coli 055:B5. Wśród lipopolisacharydów pałeczek gatunku B. fragilis największą aktywność w reakcji z odczynnikiem LAL wykazał LPS szczepu wyizolowanego z ropnia trzustki.
EN
The aim of this study was to determine a biological activity of lipopolysaccharides (LPS) from clinical Bacteroides fragilis strains isolated in Poland by means of quantitative, photometric BET (LAL) method with Limulus polyphemus amoebocyte lysate and chromogenie substrate S-2423. Lipopolysaccharides were extracted from nine clinical B. fragilis strains by the procedure of Westphal and J arm (1965). Crude LPS preparations were purified with ultracentrifugation. Biological activities of bacterial endotoxins were determined by quantitative BET method with chromogenie substrate S-2423 (ENDOCHROME™ kit). Tests were performed according to the recommendations of the producer (Charles River Endosafe® Ltd., USA). E. coli 055:B5 LPS and LPS preparations from reference B. fragilis strains were applied to compare the results of examinations. Activities of endotoxins from clinical B. fragilis strains isolated in Poland determined in reaction with Limulus amoebocyte lysate were differentiated. Among endotoxins of clinical B. fragilis strains the most active was the preparation from strain cultured in the case of pancreatic ulcer (B. fragilis 80/81 LPS). Lipopolysaccharides of examined B. fragilis strains were less active in BET test than E. coli 055:B5 LPS.