Expression of CYPscc and 3B-HSD mRNA in bitch ovary after long-term exposure to zearalenone

• Autorzy: Gajecka M. , Wozny M. , Brzuzan P. , Zielonka L. , Gajecki M.
• Języki publikacji: EN

Abstrakty

EN

In the presented study, sexually immature beagle bitches were exposed per os to 50 or 75 µg/kg b.w. of zearalenone (ZEA) for 42 d, and real-time qPCR assay was performed to investigate a possible effect of the treatment on CYPscc and 3β-HSD mRNA expression in canine ovary . While the exposure of bitches to ZEA at a lower dose (50 µg/kg b.w.; 100% NOAEL) did not cause distinct effect on the expression of examined genes, the treatment with the higher dose (75 µg/kg b.w.; 150% NOAEL) resulted in over 5-fold increase in 3β-HSD mRNA level (P<0.05). The results suggest that the appearance of ZEA in bitches ovaries may trigger the accumulation of 3β-HSD in a way similar to that of pregnenolone. Further, more detailed experiments need to be performed in order to assess whether ZEA and its major metabolites, α- and β-zearalenol, are involved in transcriptional regulation of the key steroidogenic enzymes.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2011
• Tom: 55
• Numer: 4
• Opis fizyczny: p.777-780,fig.,ref.

Twórcy

autor

Gajecka M.

• Department of Veterinary Prevention and Feed Hygiene, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-718 Olsztyn, Poland

autor

Wozny M.

autor

Brzuzan P.

autor

Zielonka L.

autor

Gajecki M.

Bibliografia

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