Prevalence of biofilm formation and wide distribution of virulence associated genes among Vibrio spp. strains isolated from the Monastir Lagoon, Tunisia

• Autorzy: Mechri B. , Medhioub A. , Medhioub M.N. , Aouni M.
• Języki publikacji: EN

Abstrakty

EN

In the current study, 65 Vibrio spp. were isolated from the Monastir lagoon water, were characterized phenotypically and genotypically. In addition, we looked for the presence of three Vibrio parahaemolyticus virulence genes (tlh, trh and tdh) and ten Vibrio cholerae virulence genes (ctxA, vpi, zot, ace, toxR, toxT, tosS, toxRS, tcpA and cpP). We also investigated the antibiotic susceptibilities and the adherence ability of the identified strains to abiotic material and to biotic surfaces. The cytotoxicity activity against HeLa and Vero cell lines were also carried out for all tested strains. All Vibrio isolates were identified to the species level and produced several hydrolytic exoenzymes. The results also revealed that all strains were expressing high rates of resistance to tested antibiotics. The minimum inhibitory concentration (MIC) values showed that tetracycline and chloramphenicol were the most effective antibiotics against the tested bacteria. Vibrio alginolyticus and V. cholerae species were the most adhesive strains to both biotic and abiotic surfaces. Besides, V. alginolyticus isolates has the high levels of recombination of genes encoding V. cholerae and V. parahaemolyticus virulence factors. In vitro cytotoxic activities of several Vibrioextracellular product were also observed among HeLa and Vero cells.

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2016
• Tom: 65
• Numer: 3
• Opis fizyczny: p.307-318,fig.,ref.

Twórcy

autor

Mechri B.

• Laboratory of Contagious Diseases and Biologically Active Substances, Faculty of Pharmacy, University of Monastir, Monastir, Tunisia
• Laboratory of Aquaculture, National Institute of Marine Sciences and Technology, Monastir, Tunisia

autor

Medhioub A.

• Laboratory of Aquaculture, National Institute of Marine Sciences and Technology, Monastir, Tunisia

autor

Medhioub M.N.

• Laboratory of Aquaculture, National Institute of Marine Sciences and Technology, Monastir, Tunisia

autor

Aouni M.

• Laboratory of Contagious Diseases and Biologically Active Substances, Faculty of Pharmacy, University of Monastir, Monastir, Tunisia

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Identyfikatory

DOI

10.5604/17331331.1215610