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2016 | 65 | 3 |

Tytuł artykułu

Prevalence of biofilm formation and wide distribution of virulence associated genes among Vibrio spp. strains isolated from the Monastir Lagoon, Tunisia

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
In the current study, 65 Vibrio spp. were isolated from the Monastir lagoon water, were characterized phenotypically and genotypically. In addition, we looked for the presence of three Vibrio parahaemolyticus virulence genes (tlh, trh and tdh) and ten Vibrio cholerae virulence genes (ctxA, vpi, zot, ace, toxR, toxT, tosS, toxRS, tcpA and cpP). We also investigated the antibiotic susceptibilities and the adherence ability of the identified strains to abiotic material and to biotic surfaces. The cytotoxicity activity against HeLa and Vero cell lines were also carried out for all tested strains. All Vibrio isolates were identified to the species level and produced several hydrolytic exoenzymes. The results also revealed that all strains were expressing high rates of resistance to tested antibiotics. The minimum inhibitory concentration (MIC) values showed that tetracycline and chloramphenicol were the most effective antibiotics against the tested bacteria. Vibrio alginolyticus and V. cholerae species were the most adhesive strains to both biotic and abiotic surfaces. Besides, V. alginolyticus isolates has the high levels of recombination of genes encoding V. cholerae and V. parahaemolyticus virulence factors. In vitro cytotoxic activities of several Vibrioextracellular product were also observed among HeLa and Vero cells.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

65

Numer

3

Opis fizyczny

p.307-318,fig.,ref.

Twórcy

autor
  • Laboratory of Contagious Diseases and Biologically Active Substances, Faculty of Pharmacy, University of Monastir, Monastir, Tunisia
  • Laboratory of Aquaculture, National Institute of Marine Sciences and Technology, Monastir, Tunisia
autor
  • Laboratory of Aquaculture, National Institute of Marine Sciences and Technology, Monastir, Tunisia
  • Laboratory of Aquaculture, National Institute of Marine Sciences and Technology, Monastir, Tunisia
autor
  • Laboratory of Contagious Diseases and Biologically Active Substances, Faculty of Pharmacy, University of Monastir, Monastir, Tunisia

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Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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