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2014 | 59 | 1 |
Tytuł artykułu

PCR detection of Neospora caninum in water buffalo foetal tissues

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The seroprevalence of Neospora caninum was surveyed by an ELISA kit on two water buffalo herds of Southern Italy. Seropositive samples were detected in 47% and 59% of individuals, respectively, thus indicating high level of exposure to the parasite even if the possibility of vertical transmission cannot be excluded. Tissue samples collected from three aborted fetuses from the same herds were investigated for N. caninum presence by PCR assays targeting the 18S and the Nc5 DNA sequences, respectively. Both methods have shown the presence of N. caninum DNA in heart and brain. Sequencing of the Nc5 genomic DNA confirmed the presence of N. caninum in the samples; phylogenetic analysis of the obtained sequences showed high homology among the Neospora recovered from different samples. The present study suggests an important role of N. caninum as a possible abortive agent for water buffaloes.
Wydawca
-
Czasopismo
Rocznik
Tom
59
Numer
1
Opis fizyczny
p.1-4,fig.,ref.
Twórcy
autor
  • Department of Animal Health, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute, 2, 80055, Portici, NA, Italy
  • Department of Animal Health, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute, 2, 80055, Portici, NA, Italy
autor
  • Department of Animal Health, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute, 2, 80055, Portici, NA, Italy
autor
  • Centro di Referenza Nazionale sull’Igiene e le Tecnologie dell’Allevamento e delle Produzioni Bufaline, S.S. 18 Via delle Calabrie, 27-84131 Fuorni, SA, Italy
  • Centro di Referenza Nazionale sull’Igiene e le Tecnologie dell’Allevamento e delle Produzioni Bufaline, S.S. 18 Via delle Calabrie, 27-84131 Fuorni, SA, Italy
autor
  • Centro di Referenza Nazionale sull’Igiene e le Tecnologie dell’Allevamento e delle Produzioni Bufaline, S.S. 18 Via delle Calabrie, 27-84131 Fuorni, SA, Italy
autor
  • Department of Animal Health, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute, 2, 80055, Portici, NA, Italy
autor
  • Department of Animal Health, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute, 2, 80055, Portici, NA, Italy
autor
  • Department of Animal Health, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute, 2, 80055, Portici, NA, Italy
autor
  • Department of Animal Health, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute, 2, 80055, Portici, NA, Italy
  • Department of Animal Health, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute, 2, 80055, Portici, NA, Italy
autor
  • Department of Animal Health, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute, 2, 80055, Portici, NA, Italy
autor
  • Department of Animal Health, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute, 2, 80055, Portici, NA, Italy
Bibliografia
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Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
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