PCR detection of Neospora caninum in water buffalo foetal tissues

• Autorzy: Auriemma C. , Lucibelli M. G. , Borriello G. , De Carlo E. , Martucciello A. , Schiavo L. , Gallo A. , Bove F. , Corrado F. , Girardi S. , Amoroso M. G. , Uberti B. D. , Galiero G.
• Języki publikacji: EN

Abstrakty

EN

The seroprevalence of Neospora caninum was surveyed by an ELISA kit on two water buffalo herds of Southern Italy. Seropositive samples were detected in 47% and 59% of individuals, respectively, thus indicating high level of exposure to the parasite even if the possibility of vertical transmission cannot be excluded. Tissue samples collected from three aborted fetuses from the same herds were investigated for N. caninum presence by PCR assays targeting the 18S and the Nc5 DNA sequences, respectively. Both methods have shown the presence of N. caninum DNA in heart and brain. Sequencing of the Nc5 genomic DNA confirmed the presence of N. caninum in the samples; phylogenetic analysis of the obtained sequences showed high homology among the Neospora recovered from different samples. The present study suggests an important role of N. caninum as a possible abortive agent for water buffaloes.

Słowa kluczowe

EN

Neospora caninum; water buffalo; fetal tissue; abortion; ELISA test; polymerase chain reaction; 18S rRNA gene sequence

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 2014
• Tom: 59
• Numer: 1
• Opis fizyczny: p.1-4,fig.,ref.

Twórcy

autor

Auriemma C.

• Department of Animal Health, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute, 2, 80055, Portici, NA, Italy

autor

Lucibelli M. G.

• Department of Animal Health, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute, 2, 80055, Portici, NA, Italy

autor

Borriello G.

• Department of Animal Health, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute, 2, 80055, Portici, NA, Italy

autor

De Carlo E.

• Centro di Referenza Nazionale sull’Igiene e le Tecnologie dell’Allevamento e delle Produzioni Bufaline, S.S. 18 Via delle Calabrie, 27-84131 Fuorni, SA, Italy

autor

Martucciello A.

• Centro di Referenza Nazionale sull’Igiene e le Tecnologie dell’Allevamento e delle Produzioni Bufaline, S.S. 18 Via delle Calabrie, 27-84131 Fuorni, SA, Italy

autor

Schiavo L.

• Centro di Referenza Nazionale sull’Igiene e le Tecnologie dell’Allevamento e delle Produzioni Bufaline, S.S. 18 Via delle Calabrie, 27-84131 Fuorni, SA, Italy

autor

Gallo A.

• Department of Animal Health, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute, 2, 80055, Portici, NA, Italy

autor

Bove F.

• Department of Animal Health, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute, 2, 80055, Portici, NA, Italy

autor

Corrado F.

• Department of Animal Health, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute, 2, 80055, Portici, NA, Italy

autor

Girardi S.

• Department of Animal Health, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute, 2, 80055, Portici, NA, Italy

autor

Amoroso M. G.

• Department of Animal Health, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute, 2, 80055, Portici, NA, Italy

autor

Uberti B. D.

• Department of Animal Health, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute, 2, 80055, Portici, NA, Italy

autor

Galiero G.

• Department of Animal Health, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute, 2, 80055, Portici, NA, Italy

Bibliografia

• Al Amri A., Senok A.C., Ismaeel A.Y., Al-Mahmeed A.E., Botta G.A. 2007. Multiplex PCR for direct identification of Campylobacter spp. in human and chicken stools. Journal of Medical Microbiology, 56, 1350–1355. DOI: 10.1099/jmm.0.47220-0.
• Baszler T.V., Gay L.J.C., Long M.T., Mathison B.A. 1999. Detection by PCR of Neospora caninum in fetal tissues from spontaneous bovine abortions. Journal of Clinical Microbiology, 37, 4059–4064.
• Chryssafidis A.L., Soares R.M., Rodrigues A.A.R., Carvalho N.A.T., Gennari S.M. 2011. Evidence of congenital transmission of Neospora caninum in naturally infected water buffalo (Bubalus bubalis) foetus from Brazil. Parasitology Research, 108, 741–743. DOI: 10.1007/s00436-010-2214-2.
• De Carlo E., Re G.N., Letteriello R., Del Vecchio V., Giordanelli M.P., Magnino S., Fabbi M. Bazzocchi C., Bandi C., Galiero G. 2004. Molecular characterisation of a field strain of bubaline herpesvirus isolated from buffaloes (Bubalus bubalis) after pharmacological reactivation. Veterinary Record, 154, 171–174.
• Dubey J.P., Romand S., Hilali M., Kwok O.C.H., Thullies P. 1998. Seroprevalence of antibodies to Neospora caninum and Toxoplasma gondii in water buffaloes (Bubalus bubalis) from Egypt. International Journal of Parasitology, 28, 527–529.
• Dubey J.P. 2003. Review of Neospora caninum and neosporosis in animals. Korean Journal of Parasitology, 41, 1–16. DOI: 10.3347/kjp.2003.41.1.1.
• Fuchs M., Hübert P., Detterer J., Rziha H.J. 1999. Detection of bovine herpesvirus type 1 in blood from naturally infected cattle by using a sensitive PCR that discriminates between wild-type virus and virus lacking glycoprotein. European Journal of Clinical Microbiology, 37, 2498–2507.
• Gonzàlez L., Buxton D., Atxaerandio R., Aduriz G., Maley S., Marco J.C., Cuervo L.A. 1999. Bovine abortion associated with Neospora caninum in Northern Spain. Veterinary Record, 144, 145–150. DOI: 10.1136/vr.144.6.145.
• Guarino A., Fusco G., Savini G., Di Francesco G., Cringoli G. 2000. Neosporosis in water buffalo (Bubalus bubalis) in Southern Italy. Veterinary Parasitology, 91, 15–21. DOI: 10.1016/S0 304-4017(00)00239-9.
• Konrad J.L., Moore D.P., Crudeli G., Caspe S.G., Cano D.B., Leunda M.R., Lischinsky L., Regidor-Cerrillo J., Odeón A.C., Ortega-Mora L.M., Echaide I., Campero C.M. 2012. Experimental inoculation of Neospora caninum in pregnant water buffalo. Veterinary Parasitology, 187, 72–78. DOI: 10.1016/j.vetpar.2011.12.030.
• Magnino S., Vigo P.G., Bandi C., Rosignoli C., Boldini M., Vezzoli F., Alborali L., Cammi G., Foni E., Colombo N., Colombo M., Bergami C., Mellini A., Fabbi M., Genchi C. 2000. Neosporosi bovina in Italia: un biennio di attività diagnostica. Selezione Veterinaria, S15–S23.
• Marianelli C., Tarantino M., Astarita S., Martucciello A., Capuano F., Galiero G. 2007. Molecular detection of Leptospira species in aborted foetuses of water buffalo. Veterinary Record, 161, 310–312.PubMedCrossRefGoogle Scholar
• Meenakshi Sandhu K.S., Ball M.S., Kumar H., Sharma S., Sidhu P.K., Sreekumar C., Dubey J.P. 2007. Seroprevalence of Neospora caninum antibodies in cattle and water buffaloes in India. Journal of Parasitology, 93, 1374–1377. DOI: 10.1645/GE-1317.1.
• OIE 2011. Chapter 2.9.7 “Listeria monocytogenes”, Chapter 2.9.9 ”Salmonellosis”, Manual of Diagnostic Tests and Vaccines for Terrestrial Animals.
• Ossewaarde J.M., Meijer A. 1999. Molecular evidence for the existence of additional members of the order Chlamydiales. Microbiology, 145, 411–417. DOI: 10.1099/13500872-145-2-411.
• Parisi A., Fraccalvieri R., Cafiero M., Miccolupo A., Padalino I., Montagna C., Capuano F., Sottili R. 2006. Diagnosis of Coxiella burnetii-related abortion in Italian domestic ruminants using single-tube nested PCR. Veterinary Microbiology, 118, 101–106. DOI: 10.1016/j.vetmic.2006.06.023.
• Rodrigues A.A., R. Gennari S.M., Aguiar D.M., Sreekumar C., Hill D.E., Miska K., Vianna M.C.B., Dubey J.P. 2004. Shedding of Neospora caninum oocysts by dogs fed tissues from naturally infected water buffaloes (Bubalus bubalis) from Brazil. Veterinary Parasitology, 124, 139–150. DOI: 10.1016/j.vetpar.2004.07.007.
• Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011. Mega5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution, 28, 2731–2739. DOI: 10.1093/molbev/msr121.
• Vemulapalli R., Mcquiston J.R., Schurig G.G., Sriranganathan N., Halling S.M., Boyle S.M. 1999. Identification of an IS711 element interrupting the wboA gene of Brucella abortus vaccine strain RB51 and a PCR assay to distinguish strain RB51 from other Brucella species and strains. Clinical and Diagnostic Laboratory Immunology, 6, 760–764.
• Vilcek S., Herring A.J., Herring J.A., Nettleton P.F., Lowings J.P., Paton D.J. 1994. Pestiviruses isolated from pigs, cattle and sheep can be allocated into at least three genogroups using polymerase chain reaction and restriction endonuclease analysis. Archives of Virology 136, 309–323.
• Yamage M., Flechtner O., Gottstein B. 1996. Neospora caninum: specific oligonucleotide primers for the detection of brain “cyst” DNA of experimentally infected nude mice by the polymerase chain reaction (PCR). Journal of Parasitology, 82, 272–279.