The aim of the study was to evaluate the Epsilometer-test (E-test) method to detect the susceptibility to amikacin and amoxicillin-clavulanic acid of 100 Staphylococcus species isolated from sheep and goats with subclinical mastitis. Of all the strains isolated, 24 were identified as Staphylococcus epidermidis, 17 as S. intermedius, 12 as S. xylosus, ten as S. warneri, nine as S. saprophyticus, nine as S. capitis, five as S. simulans, five as S. haemolyticus, three as S. sciuri, two as S. auricularis, two as S. hyicus, one as S. cohnii, and one as S. caprae. All isolates were sensitive to both antibiotics. While the MIC values of amoxicillin-clavulanic acid for the goat strains ranged from 0.125 to 1 µg/mL and of amikacin from 0.25 to 12 µg/mL, the MIC values for the sheep strains ranged from 0.047 to 2 µg/mL (amoxicillin-clavulanic acid) and 0.75 to 6 µg/ml (amikacin). The results of this study have shown that both antibiotics can be highly effective against Staphylococcus sp. that are often the reason for subclinical mastitis in small ruminants.