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2009 | 53 | 3 |

Tytuł artykułu

Usefulness of the E-test for the determination of the susceptibility of Staphylococcus sp. isolated from milk of sheep and goats with subclinical mastitis to amikacin and amoxicillin-clavulanic acid

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The aim of the study was to evaluate the Epsilometer-test (E-test) method to detect the susceptibility to amikacin and amoxicillin-clavulanic acid of 100 Staphylococcus species isolated from sheep and goats with subclinical mastitis. Of all the strains isolated, 24 were identified as Staphylococcus epidermidis, 17 as S. intermedius, 12 as S. xylosus, ten as S. warneri, nine as S. saprophyticus, nine as S. capitis, five as S. simulans, five as S. haemolyticus, three as S. sciuri, two as S. auricularis, two as S. hyicus, one as S. cohnii, and one as S. caprae. All isolates were sensitive to both antibiotics. While the MIC values of amoxicillin-clavulanic acid for the goat strains ranged from 0.125 to 1 µg/mL and of amikacin from 0.25 to 12 µg/mL, the MIC values for the sheep strains ranged from 0.047 to 2 µg/mL (amoxicillin-clavulanic acid) and 0.75 to 6 µg/ml (amikacin). The results of this study have shown that both antibiotics can be highly effective against Staphylococcus sp. that are often the reason for subclinical mastitis in small ruminants.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

53

Numer

3

Opis fizyczny

p.401-405,fig.,ref.

Twórcy

autor
  • Department of Microbiology, Faculty of Veterinary Medicine, Ataturk University, 25700, Erzurum, Turkey
autor
  • Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, Mustafa Kemal University, 31040, Hatay, Turkey
autor
  • Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, Mustafa Kemal University, 31040, Hatay, Turkey
  • Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, Mustafa Kemal University, 31040, Hatay, Turkey

Bibliografia

  • 1. Adwan G.: Antibiotic resistance against staphylococcal isolates recovered from subclinical mastitis in the North of Palestine. Islamic Univ J 2006, 14, 1-9.
  • 2. Bergonier D., De Cremoux R., Rupp G., Lagriffoul G., Berthelot X.: Masititis of dairy small ruminants. Vet Res 2003, 34, 689-716.
  • 3. Bochev I., Russenova N.: Resistance of Staphylococcus spp. strains isolated from goats with subclinical mastitis. Bulg J Vet Med 2005, 8, 109-118.
  • 4. Cercenado E., Marin M., Rico M., Vicente T., Bouza E.: Evaluation of direct E- test on lower respiratory tract samples: a rapid and accurate procedure for antimicrobial susceptibility testing. Diagn Microbial Infect Dis 2007, 8, 211-216.
  • 5. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing; 16th informational supplement. CLSI document M100-S16. Wayne, Pennsylvania, USA, 2006.
  • 6. Contreras A., Luengo C., Sanchez A., Corrales J.C.: The role of intramammary pathogens in dairy goats. Livestock Prod Sci 2003, 79, 273-283.
  • 7. Contreras A., Sanchez A., Corrales I.C., Sierra D., Gonzalo C., Paape M.J.: Mastitis in small ruminants. In: Mastitis in Dairy Production: Current Knowledge and Future Solutions. Edited by Hogeween H., Wageningen Academic Publishers, 2005, pp. 67-74.
  • 8. Fthenakis G.C.: Prevalence and etiology of subclinical mastitis in ewes of southern Greece. Small Rumin Res 1994, 13, 293-300.
  • 9. Galani I., Kontopidou F., Souli M., Rekatsina P.D., Koratzanis E., Deliolanis J., Giamarellou H.: Colistin susceptibility testing by E-test and disk diffusion methods. Int I Antimicrob Agents 2008, 31, 434-439.
  • 10. Gul M., Sensoy A., Cetin B., Korkmaz F., Seber E.: Investigation of susceptibility of nosocomial Pseudomonas aeruginosa strains against ceftazidime by E-test and disc diffusion method. Türk Mikrobiyol Cem Derg 2004, 34, 33-36.
  • 11. Hull G.S., Patshall S.: Use of the concentration gradient diffusion assay (E-test) for susceptibility testing of anaerobes, fungi, and Mycobacterium spp. Clin Microbiol Newsletter, 2002, 24, 105-109.
  • 12. Jeffrey L., Watts J.L., Yancey R.J.: Identification of veterinary pathogens by use of commercial identification systems and new trends in antimicrobial susceptibility testing of veterinary pathogens. Clin Microbiol Rev 1994, 7, 346-356.
  • 13. Kloos W.E., Wolfshohl J.F.: Identification of Staphylococcus species with the API STAPH-IDENT System. I Clin Microbiol 1982, 16, 509-516.
  • 14. Koneman E.W., Allen S.D., Janda W.M., Schreckenberger P.C., Winn W.C.: Color Atlas and Textbook of Diagnostic Microbiology, Philadelphia, Lippincott-Raven Publishers, 1997, pp. 539-566.
  • 15. Leitner G., Merin U., Silanikove N.: Changes in milk composition as affected by subclinical mastitis in goats. J Dairy Sci 2004, 87, 1719-1726.
  • 16. Lüthje P., Schwarz S.: Antimicrobial resistance of coagulase-negative staphylococci from bovine subclinical mastitis with particular reference to macrolide-lincosamide resistance phenotypes and genotypes. J Antimicrob Chemother 2006, 57, 966-969.
  • 17. McDougall S., Pankey W., Delaney C., Barlowb J., Murdoughb P.A., Scruton D.: Prevalence and incidence of subclinical mastitis in goats and dairy ewes in Vermont, USA. Small Rumin Res 2002, 46, 115-121.
  • 18. Moroni P., Vellere F., Antonini M., Pisoni G., Ruffo G., Carli S.: Antibiotic susceptibility of coagulase- negative staphylococci isolated from goats' milk. Int J Antimicrob Agents 2004, 23, 637-640.
  • 19. Moroni P., Pisoni G., Antonini M., Ruffo G., Carli S., Varisco G., Boettcher P.: Subclinical mastitis and antimicrobial susceptibility of Staphylococcus caprae and Staphylococcus epidermidis isolated from two Italian goat herds. J Dairy Sci 2005, 88, 1694-1704.
  • 20. Murat Y., Ersin I., Burcu A., Nilgun U.: Comparison of disc diffusion and E test® for in vitro antimicrobial susceptibility testing of Campylobacter jejuni and Campylobacter coli isolated from poultry. Revue Méd Vét 2005, 10, 156-159.
  • 21. Pengov A., Ceru S.: Antimicrobial drug susceptibility of Staphylococcus aureus strains isolated from bovine and ovine mammary glands. J Dairy Sci 2003, 86, 3157-3163.
  • 22. Pyorala S.: Indicators of inflammation in the diagnosis of mastitis. Vet Res 2003, 34, 565-578.
  • 23. Schalm O.W., Noorlander D.O.: Bovine Mastitis. Lea and Febiger, Philadelphia, 1957, pp. 95-123.
  • 24. Silva E.R., Siqueira A.P., Martins J.C.D., Ferreira W.P.B., Silva N.: Identification and in vitro antimicrobial susceptibility of Staphylococcus species isolated from goat mastitis in the Northeast of Brazil. Small Rumin Res 2004, 55, 45-49.
  • 25. Speakman A.J., Binns S.H., Dawson S., Hart C.A., Gaskell R.M.: Antimicrobial susceptibility of Bordetella bronchiseptica isolates from cats and a comparison of the agar dilution and E-test methods. Vet Microbiol 1997, 54, 63-72.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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