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Improvement of medium for Miscanthus x Giganteus callus induction and plant regeneration

100%

Plazek A., Dubert F.

Acta Biologica Cracoviensia. Series Botanica

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2010

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tom 52

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nr 1

105-110

Miscanthus × giganteus tissue cultures brown quickly and strongly, probably as an effect of oxidation of phenolics produced by plant tissue. The aim of the work was to improve the medium composition for callus induction and plant regeneration of M. giganteus, with two experiments. The first one was aimed at developing a protocol to inhibit biosynthesis and oxidation of phenolics. Callus was induced from immature inflorescences on basal MS medium with 6.5 mg · dm-3 2,4-D, 0.25 mg · dm-3 BAP, 500 mg · dm-3 casein hydrolysate and 30 g · dm-3 sucrose (control medium), and this medium supplemented with one of the following: 200 mg · dm-3 chitosan, 65 g · dm-3 banana pulp (BP), 100 mg · dm-3 cysteine, or 30 g · dm-3 honey instead of sucrose. Plant regeneration used basal MS medium supplemented with 30 g · dm-3 sucrose and 0.2 mg · dm-3 BAP or 0.05 mg · dm-3 KIN. The second experiment was to verify whether BP and honey increase callus production and plant regeneration. For callus induction the explants were put on the control medium and medium supplemented with one of these: 65 g · dm-3 BP, 30 g · dm-3 honey instead of sucrose, or 65 g · dm-3 BP + 30 g · dm-3 honey instead of sucrose. The regeneration medium was basal MS medium supplemented with 0.05 mg · dm-3 KIN and 30 g · dm-3 sucrose or 30 g · dm-3 honey. Tissue browning was independent of medium content, but there was less browning on medium supplemented with honey. In the first experiment, regenerated plants were obtained only on basal MS medium with 30 g · dm-3 sucrose and 0.05 mg · dm-3 KIN. In the second experiment, MS medium containing BP and honey instead of sucrose was the best medium for callus induction, and regeneration from these calli was highest on basal MS with KIN and honey.

2

Relationship between nematodes and some soil properties in the rhizosphere of banana plants

80%

Al-Ghamdi A. A. M.

International Letters of Natural Sciences

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2021

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tom 82

3

Augmenting in vitro shoot multiplication with growth regulators and light conditions in Musa acuminata cv. Dwarf Cavendish

80%

Rout G. R., Samantaray S., Das P.

Acta Biologica Cracoviensia. Series Botanica

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2001

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tom 43

An efficient protocol was developed for in vitro mass propagation of the commercial cultivar Musa acuminata cv. Dwarf Cavendish through shoot apices culture. Shoot apices with 2-3 pairs of leaf primordia were induced from sliced rhizome explants on Murashige and Skoog (1962) medium supplemented with 6.0 mg/l BA, 150 mg/l Ads and 3% (w/v) sucrose. Multiple shoots were induced from meristematic domes on the same medium. Addition of 1.0-1.5 mg/l IAA to the culture medium and incubation in continuous light (24 h) increased the yield of multiple shoots. The high multiplication coefficient was maintained stable up to the 5th subculture; thereafter it declined. Rooting was readily achieved upon transferring the shoots onto half-strength MS medium supplemented with 500 mg/l activated charcoal and 2% (w/v) sucrose. Micropropagated plantlets were hardened in a polyethylene house and successfully established in soil. There was no morphological variation among the micropropagated plants.

Ograniczanie wyników

2 Acta Biologica Cracoviensia. Series Botanica

1 International Letters of Natural Sciences

1 Al-Ghamdi A. A. M.

1 Das P.

1 Dubert F.

1 Plazek A.

1 Rout G. R.

1 Samantaray S.

1 2021

1 2010

1 2001

Improvement of medium for Miscanthus x Giganteus callus induction and plant regeneration

Relationship between nematodes and some soil properties in the rhizosphere of banana plants

Augmenting in vitro shoot multiplication with growth regulators and light conditions in Musa acuminata cv. Dwarf Cavendish