Bacteriostatic activity expressed by MIC values of S. aureus 209P was compared with MIC values for B. subtilis 6633 on 26 propolis extracts. Extracts with different MIC values ranging from a 0.060 to 0.600 mg/ml basis were tested. The determined MIC values for both species of bacteria were found to be identical in 19 samples of propolis extracts. Small differences in MIC values were observed in 7 samples. The differences did not exceed 0.010 mg/ml in 3 and 0.020 mg/ml of medium in 4 samples. The results showed that for the determination of bacteriostatic activity of propolis extracts the application of B. subtilis 6633 yielded an identical value to S. aureus 209P.
Studies on the antibacterial action of 592 samples of propolis from various regions of Poland showed that ethanolic extracts of propolis differ by their antibacterial activity. The minimal inhibitory concentration (MIC) of the extracts ranged from 60 to 490 µg/ml and the minimal bactericidal concentration (MBC) ranged from 110 to 1680 µg/ml for Staphylococcus aureus 209P (Oxford). Samples of propolis of a determined bacteriostatic activity differ clearly by the MBC values. Therefore, standardization of propolis should be based both on the MIC and MBC values. Authors suggest that the most active propolis should characterize the MIC below 300 µg/ml and the MBC not more than 450 µg/ml. The methods of extraction and antibacterial activity determination have to be strictly defined because they affect both the MIC and MBC of propolis.
W badaniach oceniono aktywność bakteriostatyczną wyrażoną wartościami MIC w stosunku do S. aureus 209P dla 10 prób olejków eterycznych otrzymanych z ekstraktów propolisu metodą seryjnych rozcieńczeń na podłożu stałym. Badane olejki wykazały zróżnicowaną aktywność bakteriostatyczną o wartościach MIC 220 - 640 nl/ml podłoża. Ponadto wykazano, że wielogodzinne gotowanie w wodzie ekstraktu propolisu jest korzystne dla jego oddziaływania bakteriobójczego (na wskutek uwalniania związków lotnych z parą wodną).
Исследовали „in vitro” чувствительность 75 бактериальных штаммов к экстракту прополиса. Среди 75 штаммов, 69 происходило (из вида Staphylococcus aureus и Streptococcus agalactiae) из проб молока, изолированных от коров с воспалением вымени. Полученные результаты исследований указывают на большую чувствительность „местных” штаммов Staphylococcus aureus и Streptococcus agalactiae к экстракту прополиса. Эта чувствительность близка или даже больше стандартной штамма золотистого стафилококка 209Р (Oxford).
Bacteriostatic activity against Staphylococcus aureus of eleven single flavonoids, pinostrobin chalcone, cinnamic acid and four of its derivatives, hydrocinnamic acid, benzoic acid and six of its derivatives and vanillin occurring in propolis has been determined quantitatively by the method of serial dilutions. Moreover, the determination of bacteriostatic activity has been performed using ethanolic extract of propolis as well as that of three fractions obtained separately from propolis and identified earlier by GC-MS method. These were the following fractions: 1) the water fraction, 2) the fraction volatile with water vapour, 3) the flavonoids fraction. Bacteriostatic activities were also established for four flavonoids and eight organic acids using BIO method and the results of the used methods are compared and discussed. The highest bacteriostatic activity against Staphylococcus aureus displayed the following flavonoids: kaempferid, galangin, apigenin, quercetin, rhamnetin, pinocembrin and kaempherol.
Провели лечение у 146 коров острых воспалительных состояний вымени с применением препарата экстракта прополиса. На 146 случаев мастита излечили 125 т.е. 86,6% общего числа леченных. Полное излечение плучили: в 100% при инфекции Candida albicans, 85% — на фоне Е. coli, 91% инфекций на фоне Staphylococcus и 84,3% инфекций Streptococcus sp. Показали также, что прополис является высокоэффективным препаратом в лечении инфекции вымени на фоне антибиотикоустойчивых микроорганизмов.