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The investigations were conducted to determine the effect of soil contamination with heavy metals (cadmium, copper, nickel, lead and zinc) on the level corresponding to 3rd pollution degree in the IUNG classification on interactions between individual aphidophagous groups and their stages of development occurring in Aphis fabae Scop. colonies on broad beans. The following were analysed: the occurrence and number of individual stages of predator development (eggs, larvae, pupae and adult specimens). The Agrell’s index of coexistence were computed from the achieved results. The values of the Agrell’s index for broad bean were low for individual predator groups and their development stages. Among the investigated predator groups, the best mutual tolerance was observed for Syrphidae and Coccinellidae. Co-existence of individual aphidophagous groups seems to be strongly conditioned by food resources, i.e. aphid availability. Heavy metals, by affecting the host plant, modified the degree of its colonization by aphids and their number, hence changes in the incidence of predators. Thus, soil pollution with zinc and nickel contributed to limiting the extent of broad bean colonization by aphids and predators, and consequently to a decrease in the values of their coexistence indices; on the other hand, soil contamination with cadmium and lead favours plant colonization by aphids and predators and therefore the presence of individual aphidophagous groups was noted more frequently on the same plants.
Spodoptera exigua, beet armyworm is an agricultural insect pest in tropical and subtropical areas of the world. Long-lasting contact with heavy metals could change sensitivity of insects to other stressor, such as insecticide. Spinosad is a nerve poison and it kills pests that it contacts and/or ones that consume it. Spinosad overstimulates nerve cells by prolonging electrical impulses by acting like acetylcholine. The aim of our study was to compare activity of larvae of S. exigua and sensitivity of them before and after bioinsecticide (spinosad) application. We used two strains of S. exigua: exposed over 100 generations to sub-lethal concentration of cadmium (44 mg Cd/kg dry weight of larval diet) and kept in standardised conditions. Stimulated locomotor activity was recorded during open-field test. Five insects, placed in separate holes in transparent lucite plate, were simultaneously observed. The analysis was made with freely available SwisTrack software that allows simultaneous tracking of more than one insect. For measurement of acetylcholinesterase activity we used acetylthiocholine iodide as a substrate. We found no differences in insect activity between control group and groups treated with spinosad in both breeding strains of S. exigua. We found differences in the changes of the acetylcholinesterase activity between the treated and no treatment groups in control strain of S. exigua, but not in the cadmium strain.
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