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In this study, the antimicrobial properties of essential oils obtained from Coriandrum sativum, Foeniculum vulgare Miller, Salvia triloba, Laurus nobilis L., Citrus limon and Origanum smyrnaeum L. were investigated. A total of eight microbial organisms belonging to six species of bacteria, namely Salmonella typhimurium, Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Escherichia coli and Enterobacter aerogenes, as well as two fungi, Candida albicans and Aspergillus niger, were studied using a disc-diffusion and agar dilution (minimal inhibition concentration) method. The antimicrobial activity of essential oils obtained from the six plants turned out to be more effective in the case of bacteria than against fungi. The antimicrobial activity against Gram-positive bacteria was more pronounced than against Gram-negative ones. All the investigated plants are known as having healing properties and are used to treat various diseases. The essential oils obtained from L. nobilis and O. smyrnaeum showed the highest antifungal activity against C. albicans and A. niger, while the essential oils obtained from F. vulgare showed the highest antimicrobial activity against P. aeruginosa and E. coli. On the other hand, the essential oils obtained from O. smyrnaeum showed stronger antibacterial activity in the case of E. aerogenes and S. aureus, but were not equally effective against E. coli. The other crude essential oils showed varied levels of antibacterial and antifungal activity. The minimal inhibition concentrations (MIC ) of the essential oils obtained from O. smyrnaeum and of those obtained from L. nobilis ranged from 1.17 to 4.71 mg/ml, and 2.4 to 19.2 mg/ml, respectively.
The gas chromatography–mass spectrometry (GC–MS) analysis of essential oil obtained by hydrodistillation from the flower of Taraxacum officinale L. revealed the presence of 25 compounds with 1,3-dimethylbenzene, 1,2-dimethylbenzene, 1-ethyl-3-methylbenzene, heneicosane and tricosane as the main components.
In the framework of the performed studies, the antibacterial activity of the following fungicides was evaluated: Miedzian 50 WG (active substance - a.s. 50% copper oxychloride), Ridomil MZ Gold 68 WG (a.s. 3.8% metalaxyl-M and 64%, mancozeb), Euparen Multi 50 WG (a.s. 50% tolylfluanid), Captan 80 WG [a.s. 80% N-(captan)], Dithane Neotec 75 WG (a.s. 75% mancozeb). The evaluation also concerned the essential oils: lavender, sage, lemon balm, clove, and a preparation based on thyme oil (BioZell). Each preparation and compound was tested against the following bacterial pathogens: Erwinia amylovora, Xanthomonas arboricola pv. corylina, X. arboricola pv. juglandis, Pseudomonas syringae pv. syringae, Agrobacterium tumefaciens (presently Rhizobium radiobacter). Each preparation and compound was tested at a concentration of 1,000 ppm of active substance. Copper oxychloride was also tested at a concentration of 1,500 ppm. Among the tested fungicides, metalaxyl-M with mancozeb, mancozeb alone, and copper oxychloride inhibited all of the tested strains of pathogenic bacteria. Tolylfluanid did not inhibit any of the bacteria used. Out of the investigated essential oils, the strongest inhibitors of bacteria were: sage, cloves, and BioZell. The protective activity of the above mentioned fungicides was also evaluated in vivo. They were assessed against fire blight on apple blossoms and pear fruitlets, against bacterial canker on sweet cherry fruitlets, and against crown gall on sunflower seedlings (the test plant). All fungicides were applied at the same concentrations as those in the in vitro tests. Only copper oxychloride was found to show protective activity against the studied diseases. This result indicates that the antibacterial properties of the other fungicides did not correspond with their activity on the plant organs used in the in vivo experiment.
Juniperus communis L. (Cupressaceae) is a plant widely cultivated in the Northern hemisphere. Juniper berries, the fruit of Juniperus communis L. are a highly valued, essential oil-rich plant material used traditionally in folk medicine as antiseptic, diuretic, antirheumatic, anti-inflammatory, antibacterial and antifungicidal agent. This paper reviews information on extraction methods of the essential oil from the juniper berries, its chemical composition and antimicrobial as well as antioxidant properties.
Eupatorium triplinerve Vahl belongs to the Asteraceae family, popularly known as Ayapana. It is a perennial shrub native to Amazon rainforests of South America. Its leaves are used through infusions, decoctions, baths, and tea. It is largely used in Brazilian folk medicine as sedative, febrifuge, stimulant, tonic and anti-inflammatory. The essential oil from aerial parts of Eupatorium triplinerve was analysis by gas chromatography mass spectroscopy (GC-MS). Thirty compounds representing 98.24 % of the oil were identified. The major compounds were 2-tert-butyl-1,4-methoxybenzene (74.3 %) and b-Selinene (8.6 %). The antibacterial activity of the essential oil was evaluated against ten bacteria (including Gram positive and Gram negative) and six phytopathogenic fungi. The oil exhibited moderated antibacterial and strong antifungal activity against all the test pathogens. The highest zone inhibition was recorded against Salamella typhae (21 mm) followed by Shigella sonnei (18 mm) at a concentration of 20μl/ disc on the other standard Ampicillin 20mg/ disc showed on average of 14-34 mm diameter of zone inhibition against the test organism. The lowest antifungal activities were recorded against Macrophomina phaseoline, and Botryodiplodia theobromae. The highest Alternaria alternate (12000 ppm). Further pharmacological and toxicology studies will be required to establish the therapeutic uses of this oil.
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