Vaginal discharge from women with acute or chronic trichomonadosis or those infected with Trichomonas asymptomatically was used for examinations. Using Best's method and Schiff's reagent after the treatment with periodic acid, the Trichomonas cytoplasm was found to contain diffused granules of glycogen; Bale's and Steedman's method revealed the presence of acid mucopolysaccharides with hyaluronic acid in the form of granules, accumulated beneath the cell membrane and along the axostyle; the use of Pappenheim-Unna and Brachet's techniques disclosed paranuclear accumulation of ribonucleinic acids; Feulgen's procedure showed the nucleus to contain granules of desoxyribonucleinic acid. No quantitative variations could be assessed in the level of the chemical substances detected in the Protozoa derived from women with various forms of trichomonadosis. The most clear-cut cytologic reactions were seen in the Protozoa from asymptomatic cases of trichomonadosis.
Rozpatrzono niektóre właściwości biologiczne rzęsistka pochwowego pod kątem, czy sprzyjają lub utrudniają zwalczanie rzęsistkowicy. Zwrócono uwagę na zdolność ruchu pierwotniaka w środowiskach o znacznej lepkości (budowa organelli ruchu i błony komórkowej), ruchu pod prąd, przenikania do przestrzeni międzykomórkowych. Podkreślono brak zdolności tworzenia cyst oraz omówiono wrażliwość form wegetatywnych na różnice ciśnienia osmotycznego środowiska, wysychanie, temperaturę i niektóre czynniki chemiczne. Zwrócono uwagę na dużą różnorodność kształtu i wielkości rzęsistka pochwowego, wiążącą się wyraźnie z klinicznymi postaciami rzęsistkowicy pochwy (wyniki własne pomiarów biometrycznych pierwotniaka). Zajęto się florą bakteryjną towarzyszącą rzęsistkowi pochwowemu w zarażeniach u ludzi i wpływem bakterii na rozwój pierwotniaka w hodowli. Wspomniano wyniki niektórych badań nad procesami przemiany materii rzęsistka i ich inhibitorami.
Women (209) with multifocal trichomonadosis were treated by oral and intravaginal administration of the drugs for 10 days. The treatment was considered to be effective when during the 3 month follow-up examinations the discharge from the vagina, uterine cervix, urethra and urinary sediment were found to contain no T. vaginalis as detected by culture method. Flagyl "Specia" was applied in 54 women in routine doses. Before the treatment the women exhibited the symptoms of acute trichomonadosis of the vagina and 29 of chronic one; in 51 cases, T. vaginalis was detected in the discharge of the urethra (in 7 of them also in the urinary sediment) and in 3 cases - in the mucus of the uterine cervix. The curative effect was observed in 50 (about 93%) of women. Aminitrozol "Polfa" was used in 95 women, orally in gelatine capsules in the dosis of 0.1 X 3 daily and intravaginally in globules in cocoa butter in the amount of 0.1 overnight. Prior to the treatment, 43 women had the symptoms of acute trichomonadosis, 37 - of chronic form and in 15 cases, T. vaginalis invasion took an asymptomatic course; in 72 cases T. vaginalis was found in the discharge of the urethra (in 13 of them also in the urinary sediment), and in 10 cases in the mucus of the uterine cervix. The healing effect was obtained in 46 (about 48%) of the women. Trichomycin "Reach'' was applied in 60 women, in tablets in the dosis of 250.000 units drally, 2 X daily and intravaginally 1 tablet overnight. Before the treatment 30 women had the symptoms of chronic vaginal trichomonadosis and 30 of acute form; in all of them, T. vaginalis was found in the urethral discharge (in 13 of them also in the urinary sediment) and in 3 cases in the uterine cervix. The healing effect was obtained in 26 (about 45%) of the women.
The specimen from the buccal cavity of 309 students was examined on Simiča medium; 75 students were found to harbour Protozoa in the orał cavity (24%), including Trichomonas tenax Dobell in 11 (4%) and Entamoeba gingivalis Brumpt in 64 (20%).
In patients with parodontium lesions infected and not infected with Trichomonas tenax the follwing indices were analysed: caries index, Russell's index (parodontium diseases), Greene-Vermillions index (oral hygiene). No statistically significant differences were found between infected and non-infected groups as regards caries and oral hygiene. Russell's index showed considerable differences between the groups of subjects.
Potassium and Lithium N-chloramide-p-azobenzenosulphonate and sodium N-chloramide benzenosulphonate (synthesized at the Department of Organic Chemistry, Łódź University, under the direction of Prof. Dr. A. Chrząszczewska) were used to treat trichomonadosis of the vagina, after the elimination of extravaginal invasion foci. At the preceding Symposium the preliminary results were reported which point to strong trichomonacidal properties of these compounds in vitro. Totally, 143 women were treated by the administration of 0.05% solutions of the compounds and of globules in cocoa butter containg 0.05 g of the substance. For 15 days the treatment consisted in vaginal cleansings with fresh compound solutions (in boiled water) and application of l globule overnight; 123 (84.6%) patients were regarded as cured in whom the 3-month follow-up examination showed no T. vaginalis. The differences in the percentage of cures following the use of potassium and lithium N-chloramide-p-azobenzenosulphonate (80%) and sodium N-chloramide benzenosulphonate (p > 95%) were not statistically significant.